[PDF] Top 20 Characterizing heterogeneity in leukemic cells using single cell gene expression analysis
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Characterizing heterogeneity in leukemic cells using single cell gene expression analysis
... in leukemic cells, we took advantage of a reference map recently identified in the normal hematopoietic system by using single-cell analysis ...the expression levels of ... See full document
13
Single-Cell Gene-Expression Analysis by Quantitative RT-PCR
... the cell types we surveyed and cardiomyocytes is the post-mitotic lifetime of the ...T cell populations in the peripheral blood are continuously renewed from the stem-cell pool in the bone ...stem ... See full document
225
Host Gene Expression Is Regulated by Two Types of Noncoding RNAs Transcribed from the Epstein-Barr Virus BamHI A Rightward Transcript Region
... carcinoma cell line AGS is a useful model for studying EBV epithelial latency since these cells consistently adopt a highly restricted pattern of expression that is quite similar to latency I in ... See full document
13
Single cell epigenomic variability reveals functional cancer heterogeneity
... chromatin using the Tn5-transposase, which preferentially inserts sequen- cing adapters into accessible chromatin ...to single cells [18, 19], this method quantifies cell-to-cell ... See full document
12
Errors during Gene Expression: Single Cell Heterogeneity, Stress Resistance, and Microbe Host Interactions
... in gene expression is dynamic and highly ...during gene expression have various effects on cell fitness and stress ...different gene expression errors lead to physiological ... See full document
9
Calcitonin receptor gene expression in K562 chronic myelogenous leukemic cells
... The expression of the human calcitonin receptor (hCTR) gene in the chronic myelogenous leukemia cell line K562 was ...K562 cells detected the presence of hCTR mRNA. Further analysis ... See full document
7
Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
... components analysis projection of the 91 cells largely segregates the five cell types (Figure ...examined expression levels for a curated list of marker mRNAs expected in serotonergic neurons, ... See full document
136
Comprehensive single cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis
... protein expression and regulation of the endogenous Ngn3 ...reporter gene (Lee et ...transgenic expression of fluorescent proteins (FPs) regulated by the Ngn3 promoter (Bechard et ...of ... See full document
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Single-cell analyses of transcriptional heterogeneity in squamous cell carcinoma of urinary bladder
... at single cells level by differentially expressed gene (DEG) analysis using NOISeq (Supplementary Table S2 and Supplementary Figure S4, probability > ...many cell cycle ... See full document
8
Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia
... Gene expression analysis revealed that Wee1 is highly expressed in adult ALL samples (n = 58, 44 at diagnosis and 14 at unpaired relapses) compared to normal mono- nuclear cells (MNCs) (p = ... See full document
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Systematic characterization of lncRNAs’ cell-to-cell expression heterogeneity in glioblastoma cells
... cellular heterogeneity and a dismal ...developed single-cell RNA-seq provides an unprecedented way for analysis of the cell-to-cell variability in lncRNA expression ...the ... See full document
12
Distinct activation thresholds of human conventional and innate-like memory T cells
... MAIT cell population (Figure 6, D and E). The single cell analysis shows a biphasic distribution within the mucosal MAIT cell population for most genes (Figure 6D, violin ...MAIT ... See full document
17
Psychrophilic proteases dramatically reduce single cell RNA seq artifacts: a molecular atlas of kidney development
... previous single-cell study of early nephrogenesis, showing that progenitors can show robust yet stochastic mRNA expression of multiple markers of later potential differentiated states (Brunskill et ... See full document
8
Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells
... stem cell populations, such as hiPSCs. Quantitative single cell gene expression profiling of hESCs and comparison of hiPSCs to this reference standard are the initial steps toward ... See full document
6
SCANPY: large scale single cell gene expression data analysis
... integrated analysis work flows for single-cell tran- scriptomic data [1] have been enabled by frameworks such as S EURAT [2], M ONOCLE [3], SCDE/P AGODA [4], MAST [5], C ELL R ANGER [6], S CATER [7], ... See full document
5
The temporal and spatial analysis of single cell gene expression
... in-depth analysis of the full data only under the ...15 cells. In the following analysis, the estimation procedure is based on complete datasets consisting of approximately 100 ... See full document
257
Single-Cell Analysis Reveals Distinct Gene Expression and Heterogeneity in Male and Female Plasmodium falciparum Gametocytes
... in expression patterns, we calculated the Pearson correlation of all genes used in this study based on their expression among 89 gametocytes, one asexual cell, and two population controls, with one ... See full document
18
Single mammalian cell gene expression analysis using microfluidics
... NF-κB gene expression dynamics with EGFP reporters, inside living mammalian ...for cells to be seeded in chambers along a microfluidic network, and subsequently grown to the desired ...to ... See full document
179
Dynamics and Heterogeneity of Gene Expression and Epigenetic Regulation at the Single-Cell Level
... target gene expression to direct recruitment and de-recruitment of four different ...when cells in the epiblast differentiate into cells in the three germ layers, pluripotency-associated genes ... See full document
147
Receptor protein tyrosine phosphatase mu expression as a marker for endothelial cell heterogeneity; analysis of RPTPmu gene expression using LacZ knock in mice
... no expression was observed in embryos at ...µ expression coincides with the formation of the intercalated discs, which de- velop late fetally or ...no expression in the skeletal ...mRNA ... See full document
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