[PDF] Top 20 cis Effects in Adeno-Associated Virus Type 2 Replication

... In order to answer the question of why SX failed to produce much encapsidated product while SB did, we tested several additional mutants. Figure 6A shows material from transfected cells processed by procedure 1 with ... See full document

... different virus types. The third compound, 5-bromo-2-deoxyuridine, is most often used for molecular biology experiments as a way to study DNA ...DNA virus that is not replication competent, ... See full document

... without replication or replication without rescue can occur (8, 12, 13, 25, 41, ...the virus life ...and replication of the AAV ...optimal replication of the AAV genome is not a ... See full document

... vitro replication at a significant level compared to a control plasmid with no ...eventually associated with a deletion of the TATA box (mutant p5D6) reduced replication to a background level that ... See full document

... Adeno-associated virus (AAV) is a defective parvovirus with unknown ...normal replication in human tissue and therefore is not readily isolated from clinical ...varicella-zoster virus ... See full document

... helper virus and the virus generally used to produce ...rAAV replication are probably not involved in AAV DNA synthesis ...AAV replication possible through regulation of cellular gene ... See full document

... The reaction mixture 100 pi contained 50 mM Trishydrochloride, pH 7.9, 2.5 mM MgC12, 2 mM DTT, 444 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The replication compl[r] ... See full document

... The adeno-associated virus 2 (AAV) contains a single-stranded DNA genome of which the terminal 145 nucleotides are palindromic and form T-shaped hairpin ...DNA replication and ... See full document

... helper virus, AAV2 infection can result in a unique form of latency featuring preferential integration into the human genome at a site termed AAVS1 at human chromosome ...integrated virus occurs when cells ... See full document

... amplicon replication in the presence of rAAV DNA and AAV rep also holds true for the respective wt viruses, we set out to analyze the influence of AAV2 coinfection on HSV-1 DNA ...rep-negative, ... See full document

... To analyze the herpes simplex virus type 1 HSV-1 functions mediating helper activity, we coinfected HeLa cells with AAV type 2 AAV-2 and different HSV-1 mutants defective in individual H[r] ... See full document

... 611 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Transfection of a pBR322-based, recombinant plasmid, pAV2, containing the entire adeno-associated virus AAV type 2 g[r] ... See full document

... nonstructural adeno-associated virus type 2 Rep proteins are known to control viral replication and thus provide the single-stranded DNA genomes required for packaging into ... See full document

... autonomous replication, even in the absence of any geno- toxic stress, but only in 293 ...Autonomous replication of AAV in 293 cells was not detected during infection studies when a multiplicity of ... See full document

... Productive replication of AAV strongly depends on helper virus functions provided by either the Ad, or herpesvirus family ...helper virus is not believed to affect rAAV entry or trafficking, in fact ... See full document

... Transactivation of the B-AAV and A-AAV capsid gene pro- moters. The constitutive activity in either 293 or HeLa cells of the AAV2 P40 promoter is very weak. The AAV2 large Rep protein is a positive transactivator of ... See full document

... AAV-mediated inhibition of Ad DNA synthesis. Previous studies indicate that Ad DNA synthesis is inhibited up to 10-fold in the presence of AAV. To determine whether inhi- bition of Ad DNA synthesis is also dose ... See full document

... adenoviral replication in the context of Ad5/AAV-2 hybrid ...harvested 2 days after transfection of HEK-293 cells with the indicated pAdEasy-AAV-2 ...adenovirus type 2 ... See full document

... for replication and production of infec- tious virus (3, 7, 11, 12, 15, 16, 31, ...DNA replication (5, 35, 38, ...DNA replication (25, 62) by binding to the inverted terminal repeats and ... See full document

... by 2 to 3 orders of magnitude ...the effects of RNA transport, stability, or transduction could also ...was associated with an apparent redistribution of the rAAV DNA from around the nucleus to ... See full document