[PDF] Top 20 Cloning of herpes simplex virus type 1 sequences representing the whole genome.

... The cloned fragments were identified by digestion of the chimeric plasmid DNA with restriction enzymes EcoRI or EcoRI and BglII followed by comparison of their electrophoretic mobilities[r] ... See full document

... upon cloning in bacteria (6, 24, 30, ...yeast cloning vector ...oriL sequences, whereas the DNA from other colonies contained approximately equal amounts of deleted and undeleted (wild-type) ... See full document

... The studies mentioned above showed complex patterns of mRNA hybridizing to several regions 749 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have examined in detai[r] ... See full document

... Table 1) confirmed and extended our previous obser- vations made using the amplicon assay ...of virus progeny, c1, c3, and c4 (substitutions of the pac2 GC element, unconserved region, and consensus se- ... See full document

... to 1% and by per- forming lysis of viral particles in two consecutive lysis buffers con- taining 1% SDS (instead of 1% sodium N-lauroylsarcosinate) and 2 mg/ml of proteinase K, ...different ... See full document

... repeat sequences are usu- ally divided into three basic types: microsatellites, mini- satellites and satellite DNA ...DNA” sequences that should perhaps be regarded as “selfish DNA”? Exploring these ... See full document

... recombinant herpes simplex virus type 1 (HSV-1) that simultaneously encodes selected structural proteins from all three virion compartments—capsid, tegument, and envelope—fused ... See full document

... 25 take the view that the accumulation of virus particles at the cell surface by interaction with heparin sulfate is followed by the interaction of the virus with a saturable receptor in[r] ... See full document

... 4 Transition from a Heterozygous to a Homozygous State of a Pair of Loci in the Inverted Repeat Sequences of the L Component of the Herpes Simplex Virus Type 1 Genome KENICHI UMENE Depar[r] ... See full document

... Genetic analysis of temperature-sensitive mutants which define the genes for the major herpes simplex virus type 2 DNAbinding protein and a new late function.. Studies of defective herpe[r] ... See full document

... We would expect such a polypeptide to migrate with an apparent size of 18,000 to 22,000 daltons, but no product this size is obviously apparent in the in vitro translation of the mix of [r] ... See full document

... after virus adsorption for 1 h at the PT contained fewer capsids in the cell nuclei than wt HSV-1-infected cells, and DNA-con- taining capsids were not observed, suggesting that ts1204 had a defect ... See full document

... of genome rearrangement specific to strain ...mutant virus d22:GFP, in which the ICP22 gene is re- placed by a GFP ...hpi, whole-cell lysates were prepared and expression of ICP22 and U S ...null ... See full document

... viral genome copies contained in individual latently infected neu- ...from whole gan- glia, assessment of the number of LAT-expressing neurons by using LAT promoter-reporter mutants, and determination of ... See full document

... McGEOCH Medical Research Council Virology Unit, Glasgow, Gll 5JR Scotland mRNA's homologous to the herpes simplex virus type 1 DNA restriction endonuclease fragment BamHI p, which contai[r] ... See full document

... In previous studies using recombinant viruses, we demonstrated that sequences between -1350 and +30 relative to the start of gC transcription regulate 13-Gal as a viral late gene and tha[r] ... See full document

... by herpes simplex virus type 1 (HSV-1), histones are present at relatively low levels on the viral ...viral genome is blocked or how histones are removed from the ... See full document

... We isolated specific HSV-1 mRNA species mapping within the region of interest by hybridization of late polyA mRNA to the appropriate restriction fragment bound to cellulose see above.. S[r] ... See full document

... MOORE,1 AND LARS HAARR`6 Department of Biochemistryl* and the Gade Institute, Departments of Pathology2 and Microbiology,6 University of Bergen, Bergen, Norway; Medical Research Council [r] ... See full document

... GELMAN, AND SAUL SILVERSTEIN* Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032 Analysis of the promoter for the herpes simple[r] ... See full document