[PDF] Top 20 Comprehensive quantification of herpes simplex virus latency at the single-cell level.
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Comprehensive quantification of herpes simplex virus latency at the single-cell level.
... This level of cellular quantification is a critical step in determining (i) viral or host cell factors which function in the establishment and maintenance of latency, (ii) the relationship ... See full document
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Role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis.
... establish latency in murine trigeminal ganglia was examined by quanti- tative PCR ...mouse single- copy adipsin gene as an internal standard ...per cell equiva- lent with an average of five copies, ... See full document
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Neuronal Subtype Determines Herpes Simplex Virus 1 Latency-Associated-Transcript Promoter Activity during Latency
... lishes latency in trigeminal ganglia (TG), while HSV-2 usually infects the genital epithe- lium and establishes latency in dorsal root ganglia (DRG) ...large-scale single-cell RNA sequencing ... See full document
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Evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear.
... human herpes simplex virus type 1 (HSV-1) establishes latency in those pe- ripheral neurons which send projections to the lytically in- fected ...the latency- associated transcripts ... See full document
6
Herpes Simplex Virus Type 1 Latency-Associated Transcript Gene Promotes Neuronal Survival
... of latency is not yet ...the level of mRNA expression, serving to down regulate viral mRNA production in neurons ...of virus production and immediate-early gene expression at the level of mRNA ... See full document
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An Immortalized Human Dorsal Root Ganglion Cell Line Provides a Novel Context To Study Herpes Simplex Virus 1 Latency and Reactivation
... a single dose of acyclovir with HSV-1 strain 17 syn ⫹ VP26-GFP at a low MOI ...Vero cell infections were scored from 14 separate experiments using a fluorescent plate reader for GFP ... See full document
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In vivo deletion analysis of the herpes simplex virus type 1 latency-associated transcript promoter.
... During herpes simplex virus latency, transcripts accumulate from a single transcription unit of the viral ...these latency-associated transcripts (LAT) has been located, and a ... See full document
7
Lund Human Mesencephalic (LUHMES) Neuronal Cell Line Supports Herpes Simplex Virus 1 Latency In Vitro
... of latency is (i) the presence of HSV genomes within a cell (or ganglion) that can be maintained without the presence of inhibitors, (ii) undetectable levels of lytic proteins, (iii) little or no lytic gene ... See full document
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Immunization with Different Viral Antigens Alters the Pattern of T Cell Exhaustion and Latency in Herpes Simplex Virus Type 1-Infected Mice
... T cell response (2, ...T cell epitope (ITAYGLVL) within the signal sequence of gK ...and virus-induced CS in ocularly infected ...the level of HSV-1 latency correlates with severity of ... See full document
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Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions
... other cell types (data not shown). A single round of infection was, thus, monitored over a 24-h period, using an MOI of 5, a statistical condition under which ...then, cell lysis was less than 5% ... See full document
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Importance of NKT Cells in Resistance to Herpes Simplex Virus, Fate of Virus-Infected Neurons, and Level of Latency in Mice
... neurovirulent virus that may approximate natural infection, these cells appear to have a critical role in determining whether the virus is con- trolled rapidly or whether there is rapid amplification and ... See full document
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Herpes Simplex Virus Type 1 Latency-Associated Transcript Reduces Human Neuroblastoma Cell Proliferation
... in cell cycle were ...evaluate cell cycle genes expression by Real Time ...relative quantification of cell cycle genes transcript levels by REST showed while the ... See full document
8
The herpes simplex virus type 1 latency-associated transcript gene regulates the establishment of latency.
... simian virus 40 (SV40) polyadenylation sequences derived from PCH110 (See ...This virus ex- presses b-gal in at least some neurons for up to 6 months ... See full document
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ICP0 Is Required for Efficient Reactivation of Herpes Simplex Virus Type 1 from Neuronal Latency
... TG cell medium and centrifuged at 1,000 rpm for 5 min in a Sorvall RT7 centrifuge (Kendro Laboratory ...Products). Cell pellets were rinsed two additional times by resuspension and ...TG cell medium. ... See full document
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Effect of ultrasound on herpes simplex virus infection in cell culture
... Howard et al. [19] reported that an adenovirus carrying the GFP marker gene could be transduced efficiently into human prostate, lung and melanoma cells via ultrasound exposure in the presence of microbubbles, but not ... See full document
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Herpes simplex virus glycoprotein D mediates interference with herpes simplex virus infection.
... Mouse LMtk- and human HEp-2 cell lines containing the gene for gD-l under control of the human metallothionein promoter II expressed various levels of gD-l constitutively and could be in[r] ... See full document
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Transcriptional targeting of herpes simplex virus for cell-specific replication.
... Generation of albumin enhancer/promoter-regulated re- combinant HSV-1 (G92A). We constructed an HSV-1 recom- binant regulated by the albumin enhancer/promoter and termed G92A (Fig. 1A). G92A is derived from d120, an ... See full document
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Structural and kinetic analyses of herpes simplex virus type 1 latency associated transcripts in human trigeminal ganglia and in cell culture
... transcripts (LATs). The LATs appear to modulate virus reactivation. In mice and rabbits the 5' origins, kinetics of synthesis, and splicing pattern of the LATs are well established. Because these details of LAT ... See full document
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Regulation and cell-type-specific activity of a promoter located upstream of the latency-associated transcript of herpes simplex virus type 1.
... By using various fragments and deletion constructs, this promoter activity 3269 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest To identify promoter regions which contr[r] ... See full document
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Protective T-Cell-Based Immunity Induced in Neonatal Mice by a Single Replicative Cycle of Herpes Simplex Virus
... Sendai virus vaccines (55) but not with live attenuated measles virus ...Sindbis virus-based vectors containing gB (19) failed (data not ...of virus able to replicate in neonates until ... See full document
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