[PDF] Top 20 Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).
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Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).
... wild type, we favor the hypothesis that these additional mutations represent an evolution of the virus to stabilize use of tRNA His as the primer for reverse ... See full document
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Nucleotide substitutions within U5 are critical for efficient reverse transcription of human immunodeficiency virus type 1 with a primer binding site complementary to tRNA(His).
... pHXB2(His-AC-TGT). Virus derived from pHXB2(His-AC-GAC) with M184V RT had slightly delayed replication compared to the virus from pHXB2(His-AC-GAC) with wild-type RT; in contrast, virus from ... See full document
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Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site.
... both tRNA and the RNA template must be prerequisite for correct annealing between these struc- tures and in order for efficient reverse transcription to ...unwinds primer tRNA in vitro ... See full document
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Genetic Analysis of a Unique Human Immunodeficiency Virus Type 1 (HIV-1) with a Primer Binding Site Complementary to tRNAMet Supports a Role for U5-PBS Stem-Loop RNA Structures in Initiation of HIV-1 Reverse Transcription
... alternative tRNA is the generation of numerous nucleotide changes in U5 following in vitro ...wild-type virus with muta- tions in U5 ...affect tRNA 3 Lys placement on ... See full document
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Transfer of Primer Binding Site-Mutated Simian Immunodeficiency Virus Vectors by Genetically Engineered Artificial and Hybrid tRNA-Like Primers
... synthetic tRNA-like primer is the primary determinant for selection and use of the tRNA species in initiation of reverse transcription in ...designed complementary tRNA x2pro , ... See full document
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The tRNA Primer Activation Signal in the Human Immunodeficiency Virus Type 1 Genome Is Important for Initiation and Processive Elongation of Reverse Transcription
... during tRNA-primed reverse transcription may result in enhanced RNase H-mediated degradation of the vRNA template, with a hot spot of cleavage at position ...PBS-bound tRNA primer stop at this ... See full document
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Structural Elements of the tRNA TΨC Loop Critical for Nucleocytoplasmic Transport Are Important for Human Immunodeficiency Virus Type 1 Primer Selection
... The solution was then collected and centrifuged at 1,000 ⫻ g for 3 min at 4°C. The supernatant was removed from the tube and immediately added to 0.2 ml of urea-sodium dodecyl sul- fate buffer (7 M urea, 0.35 M NaCl, ... See full document
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Inhibition of Human Immunodeficiency Virus Type 1 Replication with Artificial Transcription Factors Targeting the Highly Conserved Primer-Binding Site
... contains binding sites for a number of host transcription factors, including Sp1, NF- B, and Lef-1 ...(43). Binding of these factors mediates initiation of transcription by RNA polymerase II, and ... See full document
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Complementation of a primer binding site-impaired murine leukemia virus-derived retroviral vector by a genetically engineered tRNA-like primer.
... specific tRNA that is copackaged in the virion and serves as a primer for the viral reverse transcrip- tase ...priming tRNA molecule is an- nealed to the primer-binding site ... See full document
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Transcription factor binding sites downstream of the human immunodeficiency virus type 1 transcription start site are important for virus infectivity.
... infections, PBMCs were isolated from normal donors, activated with phytohe- magglutinin (Sigma, St. Louis, Mo.) (5 mg/ml) for 2 days, washed three times with RPMI, infected as described above, and maintained in complete ... See full document
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The Human Immunodeficiency Virus Type 1 Gag Polyprotein Has Nucleic Acid Chaperone Activity: Possible Role in Dimerization of Genomic RNA and Placement of tRNA on the Primer Binding Site
... the primer tRNA is annealed to the PBS on this RNA (11, 17, 23, ...kemia virus, expression of the pol gene product is unnecessary for these ... See full document
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Role of Stem B, Loop B, and Nucleotides next to the Primer Binding Site and the Kissing-Loop Domain in Human Immunodeficiency Virus Type 1 Replication and Genomic-RNA Dimerization
... of human immunodeficiency virus type 1 (HIV-1) genomic ...alternative complementary sequences, five likely to base pair only in short RNAs and four likely to base pair in ... See full document
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Inability of Human Immunodeficiency Virus Type 1 Produced in Murine Cells To Selectively Incorporate Primer
... containing tRNA were obtained only when HIV-1 Gag VLPs were produced, ...of human or mouse ...either human LysRS (hLysRS) or murine LysRS (mLysRS), both of which were tagged with ...showing ... See full document
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Leader Sequences Downstream of the Primer Binding Site Are Important for Efficient Replication of Simian Immunodeficiency Virus
... Simian immunodeficiency virus (SIV) infection of macaques is remarkably similar to that of human immunodeficiency virus type 1 (HIV-1) in humans, and the ... See full document
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Sequence Requirements for Removal of tRNA by an Isolated Human Immunodeficiency Virus Type 1 RNase H Domain
... The mismatch, BrdU mutagenesis, and substitution analyses were done with NY427 as well as HIV-1 RT. Studies with the HIV-1 RT (data not shown) have indicated that positions 3 and 6 yield alternative ... See full document
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The roles of the human immunodeficiency virus type 1 Pol protein and the primer binding site in the placement of primer tRNA(3Lys) onto viral genomic RNA.
... DNA sequencing of the PBS and its flanking region after infection of MT-2 cells by mutated viruses. MT-2 cells infected by mutated viruses (BH10-PBS2, BH10- PBS4, and BH10-PBS8), which had been treated with RNase-free ... See full document
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Mutations in the U5 Region Adjacent to the Primer Binding Site Affect tRNA Cleavage by Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo
... the tRNA between the terminal rA and the adjacent rC, 1 nucleotide from the RNA-DNA junction ...occurred 1 nucleotide from the end of the tRNA primer); however, in 9 of 62 2-LTR circles ... See full document
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Human immunodeficiency virus type 1 can use different tRNAs as primers for reverse transcription but selectively maintains a primer binding site complementary to tRNA(3Lys).
... Western blot (immunoblot) analysis. Supernatants of COS-1 cells transfected with either wild-type or mutant proviral genomes were collected 60 h posttrans- fection and filtered through a 0.45- m m-pore-size ... See full document
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Identification of a sequence within U5 required for human immunodeficiency virus type 1 to stably maintain a primer binding site complementary to tRNA(Met).
... (25). Site-directed mutagenesis was carried out by two con- secutive PCRs ...a primer in the U3 region of the 5 9 long terminal repeat (5 9 -TTGACAGCCGCCTAGC [nucleotides 8895 to 8910]) and a mutagenic ...a ... See full document
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Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication.
... During early stages of infection, the two PBS mutants, in conjunction with tRNA1Ls and tRNAPhe, appar- An early, critical step in the human immunodeficiency virus type HIV-1 life cycle i[r] ... See full document
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