[PDF] Top 20 Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
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Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... uman enterovirus D68 (EV-D68) was first isolated from sam- ples obtained in California in 1962 from four children with pneumonia and bronchiolitis ...2014 enterovirus/rhinovirus season in the ... See full document
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... the evaluation of potential candidate vaccine(s) and antiviral treatment strategies sensitive and specific viral quantitation assays are ...of real-time PCR to detect and quantitate viral ... See full document
7
Real Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages
... the development and evaluation of a real- time RT-PCR assay (NL-N assay) that can detect the four genetic lineages of hMPV described to date with comparable ...NL-N ... See full document
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Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... the real-time SYBR green I and postamplification melting temper- ature curve analysis for the mD1/TS and 3 ⴕ NC protocols using identical amplification ...multiplex, real-time reverse ... See full document
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Development and Assay of RNA Transcripts of Enterovirus Species A to D, Rhinovirus Species A to C, and Human Parechovirus: Assessment of Assay Sensitivity and Specificity of Real Time Screening and Typing Methods
... the PCR have had a major impact on the diagnosis of viral infections, often achieving greater sensitivities and shorter turnaround times than conventional assays and an ability to detect viruses refractory to ... See full document
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Development of a pan Simbu real time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
... pan-Simbu real-time RT-PCR system was able to detect all tested members of the Simbu ser- ogroup as well as most of the SBV field samples and three Bunyamwera serogroup viruses with an acceptable ... See full document
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Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... the development and validation of a more sensitive, rapid, single-reaction, one-step, multiplex real- time RT-PCR for the detection, quantitation, and differentiation of DENV-1 to -4 and CHIK ... See full document
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Optimization of a Combined Human Parechovirus Enterovirus Real Time Reverse Transcription PCR Assay and Evaluation of a New Parechovirus 3 Specific Assay for Cerebrospinal Fluid Specimen Testing
... (14) real-time RT-PCR assays were selected for clinical ...commercial real-time RT-PCR reagent kits were evaluated to determine the best-performing master mix in one- step and ... See full document
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Detection of Anaplasma marginale and A phagocytophilum in Bovine Peripheral Blood Samples by Duplex Real Time Reverse Transcriptase PCR Assay
... TaqMan-based real-time amplification (15, 20) assays were performed by use of a SmartCycler II system (Cepheid, Sunnyvale, ...duplex real-time qRT-PCR assays were developed by use of ... See full document
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Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... the evaluation of patients with upper respiratory tract ...multiplex real-time reverse transcriptase PCR (RT-PCR) assay (which uses TaqMan chemistry) and evaluated ... See full document
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Rapid Simultaneous Detection of Enterovirus and Parechovirus RNAs in Clinical Samples by One Step Real Time Reverse Transcription PCR Assay
... one-step real-time RT-PCR showed a 100% detection limit of 30 RNA copies per reaction, derived from testing 30 replicates ...step real-time RT-PCR assay for EVs was shown ... See full document
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... the development of a real-time, multiplex RT-PCR to detect various flaviviruses has been demonstrated in this study for the detection of YFV, WNV, JEV, SLEV, and DENV-1 to -4 in virus-infected ... See full document
6
Development of a Real Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
... RRT- PCR assay versus VI include RT-PCR-inhibitory substances in the samples, the use of a one-step RT-PCR method, inefficient RNA extraction procedures, and the potential for RNA to rapidly ... See full document
5
Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... immunofluorescence assay with serotype-specific monoclonal antibodies, were tested against the four primer-probe sets by both singleplex and fourplex real-time RT-PCR assays and by the nested ... See full document
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Evaluation of a Rapid and Completely Automated Real Time Reverse Transcriptase PCR Assay for Diagnosis of Enteroviral Meningitis
... for enterovirus RNA in cerebrospinal fluid (CSF) have emerged as the new gold standard for diagnosis of enteroviral meningitis, and their use can improve the management and decrease the costs for caring for ... See full document
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Evaluation of a Real Time Reverse Transcription PCR Assay for Detection of Enterovirus D68 in Clinical Samples from an Outbreak in New York State in 2014
... with enterovirus D68 (EV-D68) infection was reported in mid-August 2014 in the United ...EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that ... See full document
6
Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS- CoV) to investigate the potential of a hot start RT for improved ... See full document
5
Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... of each primer, 0.1 mM dATP, dCTP, or dGTP, 0.2 mM dUTP, 0.1 U of recombinant Tth DNA polymerase per l, 0.1 g of bovine serum albumin per l, and 0.5 M trehalose. The RT-PCR mixture was then dried within the ... See full document
6
Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... The PCR buffer contained 5-carboxy-X-rhodamine (500 nM) as the reference dye for normalization of the ...the real-time PCR ...by real-time RT-PCR with brilliant SYBR green ... See full document
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A TaqMan Probe Based Real Time PCR Assay for the Rapid Identification of the Emerging Multidrug Resistant Pathogen Candida auris on the BD Max System
... The PCR master mix was distributed in Snap-2 and Snap-3 tubes on the DNA-3 extraction ...lyophilized PCR reagent mix and the primers and probe for the sample processing control ...Max PCR cartridge ... See full document
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