[PDF] Top 20 Development of an optimized method for the detection of airborne viruses with real time PCR analysis
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Development of an optimized method for the detection of airborne viruses with real time PCR analysis
... of airborne viruses in solid impactor air sam- ...that viruses have to be eluted from the agar plates before the subsequent nucleic acid extraction step, which results in viral ...the ... See full document
6
Development and Validation of a One Step Real Time PCR Assay for Simultaneous Detection of Subtype H5, H7, and H9 Avian Influenza Viruses
... the method (22, 28), and the optimi- zation of the concentration of the multiplex PCR components to achieve optimal amplification can pose several difficulties ...the development of a multiplex assay ... See full document
5
Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... transcriptase PCR (RT- PCR), and serology (immunoglobulin M [IgM] and IgG enzyme- linked immunosorbent assays [ELISAs]) ...by PCR is the most rapid diagnostic ...several PCR-based assays have ... See full document
8
Development of a pan Simbu real time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
... obtained PCR products of the pan-Simbu real-time RT-PCR were analyzed on a ...for PCR prod- uct amplification. The assay was optimized by using a total reaction volume of 10 ... See full document
9
Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... and optimized buffer), and 2 l of template DNA in a total volume of 20 ...the PCR was initiated. UNG destroys PCR products containing ...the PCR reagents were mixed in a PCR clean ... See full document
8
Development and Validation of Multiplex One Step Real Time TaqManqRT PCR Assays for Detection and Quantification of Arboviral Encephalitis Viruses
... one-step real-time qRT-PCR assays for multiplex detection of 15 viruses, which covered nearly all the important viral pathogens that cause arboviral encephalitis, in- cluding Eastern ... See full document
39
Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... the development of a diagnostic method which enables the molecular identification of ...mismatch real-time ...the detection of mixed ...first time that such a large panel of ... See full document
6
Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... Statistical analysis based on the score numbers for these two assays indicated that no significant difference existed between the assays (P ⫽ ...dengue viruses of multiple ...with viruses of multiple ... See full document
10
Development of real time PCR for detection and quantitation of Dengue Viruses
... situations. Detection of anti-dengue IgM and IgG in the serum by ELISA is the most commonly used criteria for presumptive diagnosis of DENV ...for detection of DENV early in the course of infection for ... See full document
8
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...hands-on time. Contrasting to single probe rRT- PCR, this method introduced two different labeled probes ... See full document
7
Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real Time PCR Approach
... the real-time PCR systems to detect ...the method published by Ursi et ...these real-time PCR systems, detection limits of between 10 and 20 copies (corresponding ... See full document
5
Conventional real-time PCR-based detection of T790M using tumor tissue or blood in patients with EGFR TKI-resistant NSCLC
... In this study, we used conventional ARMS to sequence the EGFR gene of patients with NSCLC for mutations associ- ated with TKIs resistance from re-biopsied tumor DNA or from plasma ctDNA. Our results suggest that ctDNA ... See full document
6
Real Time PCR Assay for Diagnosis of Entamoeba histolytica Infection
... (zymodeme) analysis enables differentiation of ...isoenzyme analysis require a week to complete and are negative with many mi- croscopy-positive stool samples, in some cases due to delay in sample ... See full document
5
Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... Real-time RT-PCR (TaqMan) assay. Serotype-specific DEN virus primers and fluorogenic probes were designed by using the PrimerExpress, version 2.0.0 (PE Applied Biosystems, Foster City, CA), and ... See full document
7
Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients
... Sample preparation. (i) Nucleic acid extraction. For the isolation of DNA and RNA, commercially available kits were used, essentially as recommended by the manufacturer. DNA extraction from largely cell-free liquids, ... See full document
10
Real Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis
... respiratory viruses other than hBoV, and because several respiratory pathogens such as coronaviruses, rhinoviruses, enteroviruses, and the human metapneumovirus were not tested for, our data do not indicate that ... See full document
7
Detection of U S , Lelystad, and European Like Porcine Reproductive and Respiratory Syndrome Viruses and Relative Quantitation in Boar Semen and Serum Samples by Real Time PCR
... the method with GITC buff- er–phenol-chloroform and the Qiagen RNeasy kit was com- pared with the method with GITC buffer and RLT buffer supplied with the RNeasy ... See full document
9
Internally Controlled Real Time PCR Monitoring of Adenovirus DNA Load in Serum or Plasma of Transplant Recipients
... quence analysis of an approximately 450-bp fragment of the 3 ⬘ end hexon ...this method was used for genotyping the viruses. The PCR products were subjected to sequence analysis with ... See full document
7
Real Time PCR Method for Detection of Encephalitozoon intestinalis from Stool Specimens
... LC PCR testing was performed with DNA extracts from organisms known to be pathogens or normal enteric flora (50) (Table ...routine PCR for the 16S rRNA gene with universal primers and assessing the presence ... See full document
7
Development of a Real Time PCR Approach for the Specific Detection of Prunus mahaleb
... The real-time PCR assay described in this Short Report has only been tested on the control samples indicated. Whilst every effort was taken to ensure the control samples were representative of the ... See full document
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