[PDF] Top 20 Differentiation between human immunodeficiency virus type 1 (HIV 1) and HIV 2 isolates by nonradioisotopic reverse transcriptase typing assay
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Differentiation between human immunodeficiency virus type 1 (HIV 1) and HIV 2 isolates by nonradioisotopic reverse transcriptase typing assay
... We tested whether human immunodeficiency virus type 1 HIV-1 could be differentiated from HIV-2 by a reverse transcriptase RT-typing assay that measured the reduction of enzyme activity o[r] ... See full document
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An in vivo mutation from leucine to tryptophan at position 210 in human immunodeficiency virus type 1 reverse transcriptase contributes to high-level resistance to 3'-azido-3'-deoxythymidine.
... the reverse transcriptase (RT) region of 26 human immunodeficiency virus type 1 (HIV-1) isolates from eight patients treated with 3 * -azido-3 * ... See full document
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Sources and Magnitude of Intralaboratory Variability in a Sequence Based Genotypic Assay for Human Immunodeficiency Virus Type 1 Drug Resistance
... sequencing human immunodeficiency virus type 1 (HIV-1) protease (PR) and reverse transcriptase (RT) by using replicate subanalyses of 46 plasma samples ... See full document
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Mutations That Confer Resistance to Template-Analog Inhibitors of Human Immunodeficiency Virus (HIV) Type 1 Reverse Transcriptase Lead to Severe Defects in HIV Replication
... primer ⫺47 (New England Biolabs, Beverly, Mass.). For each set of reactions, equal inputs of the different enzymes (1 U each) were used. To detect single-cycle polymerization effi- ciency, an enzyme trap ... See full document
5
Characteristics and clinical significance of a stabilization assay to detect specific antibodies to reverse transcriptase of human immunodeficiency virus.
... against reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) have been detected in seropositive subjects by immunoprecipitation, Western ... See full document
7
Reduced Susceptibility of Human Immunodeficiency Virus Type 1 (HIV-1) from Patients with Primary HIV Infection to Nonnucleoside Reverse Transcriptase Inhibitors Is Associated with Variation at Novel Amino Acid Sites
... primary human immunodeficiency virus (HIV) infection have been found to harbor viral strains with reduced susceptibility to antiretroviral ...study, HIV from 16% of such ... See full document
5
Insertions in the Reverse Transcriptase Increase both Drug Resistance and Viral Fitness in a Human Immunodeficiency Virus Type 1 Isolate Harboring the Multi-Nucleoside Reverse Transcriptase Inhibitor Resistance 69 Insertion Complex Mutation
... of HIV-1 isolates harboring mutations conferring resistance to protease and RT inhibitors (PI and RTI, respectively) (for reviews, see references 8, 26, and ...that HIV-1 ... See full document
7
Comparative Analysis of Two Commercial Phenotypic Assays for Drug Susceptibility Testing of Human Immunodeficiency Virus Type 1
... Human immunodeficiency virus type 1 (HIV-1) isolates from 50 plasma specimens were analyzed for phenotypic susceptibility to licensed reverse ... See full document
5
Suppression of Human Immunodeficiency Virus Type 1 (HIV-1) Viremia with Reverse Transcriptase and Integrase Inhibitors, CD4+ T-Cell Recovery, and Viral Rebound upon Interruption of Therapy in a New Model for HIV Treatment in the Humanized Rag2−/−γc−/− Mouse
... CCR5-tropic HIV-1 JR-CSF and monitored plasma viremia and hu-CD4 ⫹ T-cell percentage over a 4-month period ...RealTime reverse transcription (RT)-PCR automated m2000 system (Abbott, Des Plaines, ... See full document
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Mutants of Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcriptase Resistant to Nonnucleoside Reverse Transcriptase Inhibitors Demonstrate Altered Rates of RNase H Cleavage That Correlate with HIV-1 Replication Fitness in Cell Culture
... The reduced replication fitness of the V106A mutant is supported by the results shown in panels B and C, which demonstrate a parallel rise in the prevalence of V179D and Y181C, respectively, when these mutants are each ... See full document
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High Degree of Interlaboratory Reproducibility of Human Immunodeficiency Virus Type 1 Protease and Reverse Transcriptase Sequencing of Plasma Samples from Heavily Treated Patients
... concordance (99.7%) was also previously reported in a multi- center study of dideoxynucleotide sequencing of cultured cell pellets (5). However, the extent of nucleotide concordance was lower in two previous multicenter ... See full document
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The Promiscuous CC Chemokine Receptor D6 Is a Functional Coreceptor for Primary Isolates of Human Immunodeficiency Virus Type 1 (HIV-1) and HIV-2 on Astrocytes
... of HIV infection of D6-expressing cells. Figure 2 shows that HIV-1 Han-2 plating on NP2/CD4/D6 cells could be blocked by increasing concentrations of RANTES and eotaxin, but not by ... See full document
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A genotypic method for determining HIV 2 coreceptor usage enables epidemiological studies and clinical decision support
... We validated the predictive performance of the geno2pheno[coreceptor-hiv2] web service on an independent test set containing nine additional V3 samples that were not contained in the data set (N = 126) that had been used ... See full document
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Identification of a Subset of Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus Strains Able To Exploit an Alternative Coreceptor on Untransformed Human Brain and Lymphoid Cells
... R5X4 isolates JAU, MLC, and ETP originated from symptom- atic patients ...the virus isolates used in this study has been previously described (46, 54) and is summarized in Table ...All virus ... See full document
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Potent Inhibition of Human Immunodeficiency Virus Type 1 Replication by Template Analog Reverse Transcriptase Inhibitors Derived by SELEX (Systematic Evolution of Ligands by Exponential Enrichment)
... for HIV-1 RT, and competitively inhibit its enzymatic activity in ...to HIV-1 RT (5). The crystal structure of HIV-1 RT bound to one of the RNA aptamers shows that the aptamer ... See full document
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Multidrug-resistant human immunodeficiency virus type 1 strains resulting from combination antiretroviral therapy.
... in 1 ml of medium in TC48 wells. On day 1, medium was aspirated, and cells were incubated at 37 8 C for 20 min with 100 m l of DEAE-dextran at 8 m g/ml plus each drug at the desired concentration in ... See full document
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Protease inhibitor associated mutations compromise the efficacy of therapy in human immunodeficiency virus – 1 (HIV-1) infected pediatric patients: a cross-sectional study
... the HIV disease was not associated with ARV treatment regimens or number of resistance ...of HIV infected children without (PR-), P < ...the HIV-1 ... See full document
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Mechanistic differences between HIV 1 and SIV nucleocapsid proteins and cross species HIV 1 genomic RNA recognition
... lentiviruses: HIV-1 [1–3] and HIV-2 ...that HIV-1 infection of humans resulted from cross-species transmission of a chimpanzee simian immunodeficiency virus ... See full document
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Primary Human Immunodeficiency Virus Type 2 (HIV-2) Isolates Infect CD4-Negative Cells via CCR5 and CXCR4: Comparison with HIV-1 and Simian Immunodeficiency Virus and Relevance to Cell Tropism In Vivo
... some HIV-2 strains is complicated by use of unidenti- fied coreceptors expressed naturally on U87/CD4 and CCC/ CD4 cells, while GHOST/CD4 cells express low levels of CXCR4 that result in background ... See full document
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Sensitive reverse transcriptase assay to detect and quantitate human immunodeficiency virus
... A sensitive biochemical assay of viral reverse transcriptase RT was developed that is useful for both the detection and quantitation of human immunodeficiency virus HIV, the agent respon[r] ... See full document
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