[PDF] Top 20 Early, Active, and Specific Localization of Herpes Simplex Virus Type 1 gM to Nuclear Membranes
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Early, Active, and Specific Localization of Herpes Simplex Virus Type 1 gM to Nuclear Membranes
... Transfected gM was indeed relocated from the TGN to the nuclear membranes during the course of infection ...than virus-expressed gM (Fig. 2 and 3). The nuclear ... See full document
14
The UL31 and UL34 Gene Products of Herpes Simplex Virus 1 Are Required for Optimal Localization of Viral Glycoproteins D and M to the Inner Nuclear Membranes of Infected Cells
... diluted 1:50 in phosphate-buffered saline (PBS) supplemented with ...and 1% fish gelatin, and (iii) these sections were reacted with goat anti-mouse immunoglobulin G (IgG) conjugated with 12-nm colloidal ... See full document
10
Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.
... amino-terminal localization region of ICP27 do not significantly affect viral DNA ...of 1. DNA was isolated from the infected cultures at 1 and 12 h after infection, and parallel infected cells were ... See full document
12
Microtubule Reorganization during Herpes Simplex Virus Type 1 Infection Facilitates the Nuclear Localization of VP22, a Major Virion Tegument Protein
... of 1:10 for immu- nofluorescence and 1:50 for ...antibody specific for VP22 has been described previously ...1A, specific for ␣-tubulin, was obtained from Sigma and was used at a dilution of ... See full document
15
Recruitment of Herpes Simplex Virus Type 1 Immediate-Early Protein ICP0 to the Virus Particle
... cytoplasmic localization would seem to be a prerequi- site for ICP0 assembly ...in specific punctate do- mains in the ...with virus lacking the VP22 gene, ICP0 failed to localize to these puncta to ... See full document
15
Role of Herpes Simplex Virus 1 Immediate Early Protein ICP22 in Viral Nuclear Egress
... cell type-dependent manner ...of specific viral genes ...HSV-1 nuclear egress regulators UL31, UL34, Us3, and UL47 in HSV-1-infected cells and colocalized with UL31 and UL34 at the ... See full document
10
Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27.
... olar localization in these cases is dependent both on an NLS and on fairly large protein regions which in some cases corre- spond to known functional ...olar localization of nucleolin is dependent on ... See full document
13
Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
... of membranes during viral infection (reviewed in references 27 and ...to nuclear and perinuclear regions in infected cells, yet the corresponding genes still result in syncytial plaques when mutated (18, ... See full document
8
Herpes Simplex Virus 1 gN Partners with gM To Modulate the Viral Fusion Machinery
... that gM expression is detectable as early as 2 hpi and that gN mRNAs are only discernible from 10 hpi onward, coincident with the release of gM from the ER (38), it was tempting to pos- tulate that ... See full document
11
Herpes simplex virus type 1 protein IE63 affects the nuclear export of virus intron-containing transcripts.
... the herpes simplex virus type 1 immediate-early protein IE63 (ICP27) affects the cellular localization of virus ...small nuclear ribonucleoprotein ...mutant ... See full document
11
A Functional Role for TorsinA in Herpes Simplex Virus 1 Nuclear Egress
... wild type redistributes pUL34 into cytoplasmic puncta that colocalize with a capsid ...the nuclear rim to the cytoplasm in PC6-3 cells overexpressing TAwt but not ...and localization of pUL34 was ... See full document
13
Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids
... Herpes simplex virus type 1 (HSV-1) virions contain a linear double-stranded DNA genome of approximately 152 kb that is packaged into an icosahedral capsid ...inner ... See full document
14
Nuclear export of VP19C is not essential for replication of herpes simplex virus type 1
... for nuclear export of VP19C, and VP19C is exported from the nucleus through the NES via a CRM1-dependent ...Rabies virus P-protein and chicken anemia virus VP3, regulate their NLS/NES activity via ... See full document
5
Formation of herpes simplex virus type 1 replication compartments by transfection: requirements and localization to nuclear domain 10.
... Preparation and analysis of cellular DNA. Total cellular DNA from trans- fected cells was isolated essentially as described previously (58). When required, cells were grown in the presence of PAA (400 mg/ml). At 18 to 24 ... See full document
10
Localization of Herpes Simplex Virus Type 1 UL37 in the Golgi Complex Requires UL36 but Not Capsid Structures
... The herpes simplex virus type 1 (HSV-1) UL37 gene encodes a 120-kDa polypeptide which resides in the tegument structure of the virion and is important for ...A virus ... See full document
8
Virion component of herpes simplex virus type 1 KOS interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186.
... 312 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Herpes simplex virus HSV strains HSV type 1 HSV-1 KOS and HSV-2 186 are representative of delayed and early shutoff [r] ... See full document
5
Cooperativity among herpes simplex virus type 1 immediate-early regulatory proteins: ICP4 and ICP27 affect the intracellular localization of ICP0.
... To identify the region of the ICPO molecule that responds to ICP27, Vero cells were cotransfected with 1 ,ug of pSH or 1 ,ug of the ICPO nonsense mutant plasmids pn428, pn525, pn680, pn7[r] ... See full document
14
Herpes simplex virus type 1-specific cytotoxic T lymphocytes recognize virus nonstructural proteins.
... 2265 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The specificity of herpes simplex virus type 1-specific cytotoxic T cells was examined with target cells expressing[r] ... See full document
9
Localization of cis-acting sequence requirements in the promoter of the latency-associated transcript of herpes simplex virus type 1 required for cell-type-specific activity.
... Our current data demonstrate that the IC-1 factor is selectively present in the human neuroblastoma cells IMR-32 compared with HeLa cells and binds a DNA sequence which is selectively re[r] ... See full document
10
The herpes simplex virus 1 UL11 proteins are associated with cytoplasmic and nuclear membranes and with nuclear bodies of infected cells.
... (diluted 1:100 in dilution ...for 1 to 2 h at 48C, pelleted by low-speed centrifugation, washed with and resuspended in PBS, dehydrated, infiltrated, and low-temperature embedded (18) in Lowicryl K4M ... See full document
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