[PDF] Top 20 Evaluation of tcdB Real Time PCR in a Three Step Diagnostic Algorithm for Detection of Toxigenic Clostridium difficile
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Evaluation of tcdB Real Time PCR in a Three Step Diagnostic Algorithm for Detection of Toxigenic Clostridium difficile
... or three-step EIA/ DPCR algorithm are justified by the earlier detection of CDI, which would reduce the number of cases resulting from noso- comial ...the three-step EIA/DPCR ... See full document
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Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease
... Simplexa Clostridium difficile universal direct PCR, a real-time PCR assay for the detection of the ...C. difficile toxin B (tcdB) gene using the 3M ... See full document
6
Comparison of BD GeneOhm Cdiff Real Time PCR Assay with a Two Step Algorithm and a Toxin A/B Enzyme Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
... these PCR-identified patients have low titers of toxin that are below the detection limits of toxin ELISA or cytotoxin neutralization ...C. difficile spores in the hospital setting, because they test ... See full document
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Comparison of Nine Commercially Available Clostridium difficile Toxin Detection Assays, a Real Time PCR Assay for C difficile tcdB, and a Glutamate Dehydrogenase Detection Assay to Cytotoxin Testing and Cytotoxigenic Culture Methods
... waiting time for a test result and the need to maintain a cell line, have contributed to the decreased availability of the CYT in diagnostic ...this evaluation, the CYT was the best performing toxin ... See full document
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Yield of Stool Culture with Isolate Toxin Testing versus a Two Step Algorithm Including Stool Toxin Testing for Detection of Toxigenic Clostridium difficile
... two-step algorithm for optimal detection of toxigenic Clostridium ...two-step algorithm, stools were screened for C. difficile-associated glutamate dehydrogenase ... See full document
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Effective Detection of Toxigenic Clostridium difficile by a Two Step Algorithm Including Tests for Antigen and Cytotoxin
... C. difficile panel (Biosite Diagnostics, San Diego, ...for toxigenic C. difficile, espe- cially as an alternative to isolating ...C. difficile in ...certain toxigenic (toxin A-positive) ... See full document
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Comparison of a Commercial Real Time PCR Assay for tcdB Detection to a Cell Culture Cytotoxicity Assay and Toxigenic Culture for Direct Detection of Toxin Producing Clostridium difficile in Clinical Samples
... compared real-time PCR assays and cytotoxin or enzyme immunoassay (EIA) testing to anaerobic toxigenic culture (3, 22, 26, 30, ...multiplex PCR assay targeting conserved regions of tcdA ... See full document
6
Evaluation of a New Automated Homogeneous PCR Assay, GenomEra C difficile, for Rapid Detection of Toxigenic Clostridium difficile in Fecal Specimens
... the detection of C. difficile tox- ins or glutamate dehydrogenase (GDH) in stool samples by im- munochromatographic antigen (IA) tests or enzyme immuno- assays (EIAs) ...than toxigenic cultures. ... See full document
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Impact of Strain Type on Detection of Toxigenic Clostridium difficile: Comparison of Molecular Diagnostic and Enzyme Immunoassay Approaches
... direct PCR testing worth the added expense of the assay? In their cost analysis, Larson et ...direct PCR testing or the three-step algorithm was justified by the earlier detec- tion of ... See full document
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Comparison of Real Time PCR for Detection of the tcdC Gene with Four Toxin Immunoassays and Culture in Diagnosis of Clostridium difficile Infection
... rapid real-time PCR method using fluorescence resonance energy transfer probes and the LightCycler (Roche Diagnostics), which will detect the presence of the tcdC gene of Clostridium ... See full document
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Perirectal Swab Surveillance for Clostridium difficile by Use of Selective Broth Preamplification and Real Time PCR Detection of tcdB
... PCR detection of toxigenic ...C. difficile. The ProGastro Cd assay kit for tcdB (Gen-Probe Prodesse) was modified to run on the ABI 7500 Fast real-time PCR system ... See full document
6
Multicenter Clinical Evaluation of the Portrait Toxigenic C difficile Assay for Detection of Toxigenic Clostridium difficile Strains in Clinical Stool Specimens
... Portrait Toxigenic C. difficile Assay within 96 h of collection if the sample was maintained at 2°C to 8°C or within 2 h if it was maintained at room temperature (15°C to 30°C), in accordance with the ... See full document
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Assessment of Clostridium difficile Infections by Quantitative Detection of tcdB Toxin by Use of a Real Time Cell Analysis System
... C. difficile toxin caused cytotoxic effects on the cells and resulted in a dose-dependent and time-dependent decrease in the electronic ...the time it takes for a clinical specimen to cause a 50% ... See full document
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Multiplex PCR Targeting tpi (Triose Phosphate Isomerase), tcdA (Toxin A), and tcdB (Toxin B) Genes for Toxigenic Culture of Clostridium difficile
... first step of this work was to test the ability of the multiplex PCR (i) to distinguish ...C. difficile from other Clos- tridium species and (ii) to distinguish between the three main ... See full document
5
Diagnosis of Clostridium difficile infection by toxigenic culture and PCR assay
... One hundred stool specimens were taken from diarrheal patients in hospitals of Babol. All patients had a history of antibiotic use, and demographics data of patients were collected via a questionnaire. The specimens were ... See full document
7
Evaluation of the cobas Cdiff Test for Detection of Toxigenic Clostridium difficile in Stool Samples
... the time of admission for the detection of ...the time of admission, patients acquire new organisms from their surrounding environment but that after the first night, they contribute bacteria ... See full document
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Indeterminate tcdB using a Clostridium difficile PCR assay: a retrospective cohort study
... complication. Three patients (all with non-severe disease) underwent endoscopy; none had evidence of pseudomembranous ...positive tcdB result using Xpert® within 30-days of the in- determinate result; ... See full document
5
Defining the Roles of TcdA and TcdB in Localized Gastrointestinal Disease, Systemic Organ Damage, and the Host Response during Clostridium difficile Infections
... that TcdB is the major factor inducing host innate immune and proinflammatory ...⫺ TcdB ⫹ deriva- tive, suggesting that TcdA also plays a role in upregulating these responses, which agrees with similar ... See full document
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Clinical and microbiologic characteristics of tcdA negative variant clostridium difficile infections
... before they had begun treatment. In 32 patients, diarrhea improved or resolved without treatment, and 85 patients (16 A - B + and 69 A + B + CDI cases) completed treatment (16/22 vs. 69/105, p = 0.623). Treatment ... See full document
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Molecular and Susceptibility Analysis of Toxigenic Clostridium difficile Obtained from Adult Patients Suspected of CDI in Trinidad
... This is an EIA screening method that detects, common C. difficile producing enzyme. This was carried out using the C. diff Quick Check Complete (Techlabs). This test kit was able to detect both the presence of the ... See full document
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