[PDF] Top 20 Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
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Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
... the virion as a component of the Gag-Pol ...of human immunodeficiency virus type 1 (HIV-1), cleavage by the viral protease occurs during viral ...budding. ... See full document
6
Role for Human Immunodeficiency Virus Type 1 Tat Protein in Suppression of Viral Reverse Transcriptase Activity during Late Stages of Viral Replication
... of virion-associated HIV-1 DNA. Virion-associated nucleic acids were extracted, and aliquots corresponding to 10 and 100 pg of p24 were subjected to quantitative PCR using the R-U5 and R-5NC primer ... See full document
9
Cloning and Expression of a Human T-Lymphotropic Virus Type 1 Protein with Reverse Transcriptase Activity
... by trans- forming an amber suppressor strain (amber-Gln ...with 1 mM isopropyl thiogalactoside. After induction, the culture was incubated for an additional 2 h, and the cells were harvested by ... See full document
6
Human Immunodeficiency Virus type 1 reverse transcriptase exists as post translationally modified forms in virions and cells
... (i) virus producer cells (Fig- ure 3A), (ii) intracellularly following HIV infection (Fig- ure 3B) and (iii) in HIV RTC's (Figure ...infectious virus and although they contain forms of HIV RT that ... See full document
12
Virion Instability of Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) Mutated in the Protease Cleavage Site between RT p51 and the RT RNase H Domain
... leukemia virus consists of a single subunit (29), suggesting that the cat- alytically active enzyme is a monomer or perhaps ...leukemia virus RT have a single subunit com- position and possess substantial ... See full document
10
Inhibition of -Primed Reverse Transcription by Human APOBEC3G during Human Immunodeficiency Virus Type 1 Replication
... cellular expression and viral incorporation of these truncated species were also reported in that paper, except for hA3G104-246, which is also incorpo- rated efficiently into virions (data not ...of ... See full document
13
Resistance to Nucleoside Analog Reverse Transcriptase Inhibitors Mediated by Human Immunodeficiency Virus Type 1 p6 Protein
... NC-TF-p6-PR expression vector in a reticulocyte lysate transcription and translation (TNT) system, did not identify changes in the rate of processing of the SPT precursor (SPT is the 3-amino-acid duplication in ... See full document
10
Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... (ts) human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) mutant was generated by charged-cluster-to-alanine ...mutant virus, containing ... See full document
8
Influence of Reverse Transcriptase Variants, Drugs, and Vpr on Human Immunodeficiency Virus Type 1 Mutant Frequencies
... Vpr virion incorporation into HIV-1 ...in trans and incorporated into virions ...for expression of the vesicular stomatitis virus G protein, and 10 g of pAS1B-Vpr (wt or ...h ... See full document
10
Mutations in the Thumb Allow Human Immunodeficiency Virus Type 1 Reverse Transcriptase To Be Cleaved by Protease in Virions
... Although human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) has been extensively studied, there are still significant questions about the ... See full document
9
The Nonnucleoside Reverse Transcriptase Inhibitor UC-781 Inhibits Human Immunodeficiency Virus Type 1 Infection of Human Cervical Tissue and Dissemination by Migratory Cells
... the human immunodeficiency virus (HIV)-AIDS epi- demic enters its third decade, there is still a desperate need to develop preventative options to help reduce the number of new HIV type ... See full document
8
Chemical barriers to human immunodeficiency virus type 1 (HIV-1) infection: retrovirucidal activity of UC781, a thiocarboxanilide nonnucleoside inhibitor of HIV-1 reverse transcriptase.
... cells during 18 h of exposure to UC781 was significantly atten- uated in infectivity compared to that from no-drug controls or cells treated with UC84 ...even after 30 days of coculture with unin- fected ... See full document
8
Sensitive Phenotypic Detection of Minor Drug Resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Variants
... (A) Expression and reverse transcription of TyHRT elements result in the conversion of a nonfunctional his3 gene (his3AI) into a functional HIS3 ...independent reverse transcription ... See full document
9
Cross-Linking of the Fingers Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase to Template-Primer
... Purification of HIV-1 RT. A single colony of Escherichia coli strain DH5␣ transformed with one of the plasmids mentioned above was inoculated into 750 ml of NZY medium and grown at 37°C for 12 to 14 h before ... See full document
11
Hypersusceptibility to Substrate Analogs Conferred by Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... verse transcriptase (RT) is a defining step in the retroviral life cycle (8) and a key target of therapy for human immunodefi- ciency virus type 1 (HIV-1) infection ... See full document
10
Human Immunodeficiency Virus Type 1 Subtype F Reverse Transcriptase Sequence and Drug Susceptibility
... inhibitor, TIBO, whereas another isolate (RO-BCI23) showed significantly diminished susceptibility to TIBO. Although this compound is not used to treat HIV infection, the phenotypic susceptibility analysis showed ... See full document
5
Mutations That Abrogate Human Immunodeficiency Virus Type 1 Reverse Transcriptase Dimerization Affect Maturation of the Reverse Transcriptase Heterodimer
... abrogate human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) dimerization on virus replication is not known, as mutations shown previously ... See full document
11
A Mutation in the 3′ Region of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase (Y318F) Associated with Nonnucleoside Reverse Transcriptase Inhibitor Resistance
... To confirm the effect of the Y318F mutation on decreased drug susceptibility, mutations in the RT-coding region were generated by site-directed mutagenesis of a wild-type HXB2-D EcoRI-PstI restriction enzyme ... See full document
5
Effects of Human Immunodeficiency Virus Type 1 Resistance to Protease Inhibitors on Reverse Transcriptase Processing, Activity, and Drug Sensitivity
... Human immunodeficiency virus type 1 (HIV-1) variants resistant to protease inhibitors often display a reduced replicative capacity as a result of an impairment of protease ...of ... See full document
5
Mutations in the Primer Grip of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Impair Proviral DNA Synthesis and Virion Maturation
... of human immunodeficiency virus type 1 reverse transcriptase (RT) on virus formation and ...mutant virus M230A were similar to those of the wild type, ... See full document
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