[PDF] Top 20 The cps Genes of Streptococcus suis Serotypes 1, 2, and 9: Development of Rapid Serotype Specific PCR Assays
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The cps Genes of Streptococcus suis Serotypes 1, 2, and 9: Development of Rapid Serotype Specific PCR Assays
... a PCR method which can be used to detect pathogenic strains of S. suis serotype 2 as well as highly pathogenic strains of ...S. suis serotype 1 (33). However, besides S. ... See full document
7
Development of Rapid Serotype Specific PCR Assays for Eight Serotypes of Streptococcus suis
... to serotype 5 were positive in the serotype 5 PCR ...The cps loci of the three serologically untypeable isolates were se- ...The serotype-specific gene (cps5I) detected in sero- ... See full document
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Rapid Visual Detection of Highly Pathogenic Streptococcus suis Serotype 2 Isolates by Use of Loop Mediated Isothermal Amplification
... Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that causes considerable economic losses to the pig industry and significantly threatens public health ... See full document
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Multiplex PCR Assays for Simultaneous Detection of Six Major Serotypes and Two Virulence Associated Phenotypes of Streptococcus suis in Tonsillar Specimens from Pigs
... a PCR based on the epf gene specifically detects virulent strains of ...S. suis serotype 2 and highly virulent strains of S. suis serotype 1 ...addition, PCR ... See full document
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Serotype-specific role of antigen I/II in the initial steps of the pathogenesis of the infection caused by Streptococcus suis
... S. suis AgI/II Bioinformatics analyses using the S. suis (taxid 1307) genome database available in GenBank revealed the presence of genes coding for AgI/II-like proteins in the genomes of ... See full document
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Development of Serotype Specific PCR Assays for Typing of Haemophilus parasuis Isolates Circulating in Southern China
... Fifteen serotypes of ...of PCR assays that are able to identify all the currently known ...parasuis serotypes, with a detection limit of 5 CFU. This PCR method is particularly useful to ... See full document
9
Development and Evaluation of Serotype and Group Specific Fluorogenic Reverse Transcriptase PCR (TaqMan) Assays for Dengue Virus
... transcriptase PCR (RT-PCR) assays were developed for serotypes 1 to 4 and group-specific detection of dengue ...virus. Serotype- and group-specific oligonucleotide ... See full document
6
Development of a PCR Method for Rapid Identification of New Streptococcus mutans Serotype k Strains
... rgpI genes have been shown to be correlated to glucose side chain formation in the serotype- specific polysaccharide of ...these genes found in the present serotype k ...rgpI ... See full document
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Novel PCR Restriction Fragment Length Polymorphism Method for Determining Serotypes or Serogroups of Streptococcus pneumoniae Isolates
... using PCR amplification of a portion within the locus between cpsA and cpsB (located immediately upstream of dexB) followed by restriction fragment polymorphism was described previously ...than serotype. ... See full document
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Multiplex PCR Assay for Detection of Streptococcus suis Species and Serotypes 2 and 1/2 in Tonsils of Live and Dead Pigs
... multiplex PCR assay allowed also the detection of all S. suis serotypes and more specifically serotypes 2 and ...a PCR test able to detect serotype 2 alone, because ... See full document
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Impact of serotype and sequence type on the preferential aerosolization of Streptococcus suis
... S. suis ST1 strains tested in this study, but not S. suis ST25 and ST28 strains, appear to be statistically preferentially ...adhesin genes are up- regulated in air. It has been shown that S. ... See full document
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Development of Real Time PCR Assays for Detection of the Streptococcus milleri Group from Cystic Fibrosis Clinical Specimens by Targeting the cpn60 and 16S rRNA Genes
... For the 16s_SA assay, a conserved region within the 16S rRNA sequence was used to design primers specific to S. anginosus ATCC 33397, while the 16s_SCI assay was designed based on conserved sequence for S. ... See full document
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Loop Mediated Isothermal Amplification (LAMP) for the Detection of Listeria monocytogenes and Major Pathogenic Serotypes
... and PCR-based protocols for the identification of pathogenic serotypes have overcome some of the standardization difficulties, but these kits are also expensive and require sophisticated equipment to ... See full document
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Evaluation of a Multiplex PCR Test for Simultaneous Identification and Serotyping of Actinobacillus pleuropneumoniae Serotypes 2, 5, and 6
... multiplex PCR test did not amplify DNA frag- ments from any of the 29 different species related to ...for serotypes 3, 4, and 7 and consequently do not involve any of the serotypes for which the ... See full document
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Distribution of Environmentally Regulated Genes of Streptococcus suis Serotype 2 among S suis Serotypes and Other Organisms
... regulated genes of Streptococcus suis strain 10 among all 35 S. suis serotypes was determined by using hybridization with the amplified genes as ...these genes among the ... See full document
8
Cloning and Characterization of the Gene Encoding the Glutamate Dehydrogenase of Streptococcus suis Serotype 2
... in 1⫻ SDS sample buffer by heating at 100°C for 5 min followed by centrifugation for 2 min to pellet cellular ...emulsified 1:1 in Fruend complete ...emulsified 1:1 with Freund’s ... See full document
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Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada
... of serotype 9 strains may be nearly 400 years old, originat- ing from a common ancestor that further evolved into 2 lineages in ...The rapid population expansion of the dominant lineage ... See full document
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Association between Serotype-Specific Antibody Response and Serotype Characteristics in Patients with Pneumococcal Pneumonia, with Special Reference to Degree of Encapsulation and Invasive Potential
... infective serotype, and as many as 24% (24/70) of the patients showed a decrease in Ig titer between paired sera (Ig titer ratio, ⬍1; Table ...addition, serotypes with a high degree of encapsulation ... See full document
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Analysis of Genetic Diversity of Streptococcus suis Clinical Isolates from Pigs in Spain by Pulsed Field Gel Electrophoresis
... S. suis, mainly of serotype 2 isolates due to the special clinical relevance of this ...S. suis swine isolates (2, 7). Infections by S. suis represent one of the most important ... See full document
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Global distribution of invasive serotype 35D Streptococcus pneumoniae post PCV13 introduction
... Global distribution of invasive serotype 35D Streptococcus pneumoniae post PCV13 introduction 1 Global distribution of invasive serotype 35D Streptococcus pneumoniae post 1 PCV13 introduction 2 Stepha[.] ... See full document
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