[PDF] Top 20 Core Genome Multilocus Sequence Typing Scheme for High Resolution Typing of Enterococcus faecium
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Core Genome Multilocus Sequence Typing Scheme for High Resolution Typing of Enterococcus faecium
... Enterococcus faecium, a common inhabitant of the human gut, has emerged in the last 2 decades as an important multidrug- resistant nosocomial ...century, multilocus sequence typing ... See full document
10
Core Genome Multilocus Sequence Typing Scheme for Stable, Comparative Analyses of Campylobacter jejuni and C coli Human Disease Isolates
... cost-effective, high-resolution typing systems. Whereas previous typing systems relied on choosing those components of the organism or parts of its genome that were amenable to ... See full document
12
Epidemiological Typing of Serratia marcescens Isolates by Whole Genome Multilocus Sequence Typing
... Next-generation sequencing (NGS) is becoming very popular in clinical microbiology (20, 21), but wgMLST for S. marcescens has not yet been described. WGS has been used to study S. marcescens virulence after wound ... See full document
16
Phylogenetic Analysis of Mycobacterium tuberculosis Strains in Wales by Use of Core Genome Multilocus Sequence Typing To Analyze Whole Genome Sequencing Data
... low sequence variation and lack of horizontal gene transfer in ...of resolution than the PGG and SCG methods and provides phylogenetic associations that can be correlated with spoligo- type families, and ... See full document
11
Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Genome Wide Typing of Clostridium difficile
... novel typing approaches, backward comparability with previous typing methods and a high level of typeability, ...used typing method for ... See full document
10
Hash Based Core Genome Multilocus Sequence Typing for Clostridium difficile
... which is problematic with large data sets that span many laboratories; hashes also overcome this limitation. We also provide the code to run the algorithms developed. This article also highlights important limitations of ... See full document
11
Multilocus Sequence Typing Scheme for Enterococcus faecalis Reveals Hospital Adapted Genetic Complexes in a Background of High Rates of Recombination
... MLST scheme al- lows both evolutionary and population structure analyses and also enables the study of ...faecalis-E. faecium interspecies ...a scheme that is too dis- criminatory for long-term and ... See full document
9
Defining a Core Genome Multilocus Sequence Typing Scheme for the Global Epidemiology of Vibrio parahaemolyticus
... cgMLST scheme that allows for fast typing of ...cgMLST scheme was tested with a diverse set of strains belonging to the same or unrelated outbreaks and was able to differentiate them accordingly, ... See full document
16
Core Genome Multilocus Sequence Typing: a Standardized Approach for Molecular Typing of Mycoplasma gallisepticum
... on sequence analysis of 5 loci remains insufficient for accurate outbreak ...massive sequence data and the diverse properties of different genes within bacterial genomes results in a lack of standard ... See full document
11
Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Whole Genome Sequence Based Typing of Listeria monocytogenes
... MLST and cgMLST both use alleles and not nucleotide poly- morphisms as units of comparison. Irrespective of the number of nucleotide polymorphisms involved, each allelic change is num- bered as a single event; i.e., an ... See full document
8
A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis
... cgMLST scheme to describe the ...cgMLST scheme was also tested to investigate the relatedness of strains putatively involved in hospital outbreak events and to obtain further insights into ...cgMLST ... See full document
12
Multilocus Sequence Typing Scheme for Enterococcus faecium
... reference scheme for the typing of E. faecium to allow unam- biguous comparison of data between different ...E. faecium by using a sample of 123 isolates originating from human and animal ... See full document
10
Transmission and genetic diversity of Enterococcus faecalis among layer chickens during hatch
... Some 25% of the isolates demonstrated abundant growth in pure culture supporting a genuine vertical transmission of E. faecalis. Such route has previously only been indicated to play a minor role during infec- tion of ... See full document
6
Comparing Pulsed Field Gel Electrophoresis with Multilocus Sequence Typing, spa Typing, Staphylococcal Cassette Chromosome mec (SCCmec) Typing, and PCR for Panton Valentine Leukocidin, arcA, and opp3 in Methicillin Resistant Staphylococcus aureus Isolates at a U S Medical Center
... In the United States, many studies have demonstrated that, since 2003, there have been 2 predominant lineages of methicillin- resistant Staphylococcus aureus (MRSA) that commonly cause hu- man infections, USA300 and ... See full document
6
Evaluation of a Stenotrophomonas maltophilia bacteremia cluster in hematopoietic stem cell transplantation recipients using whole genome sequencing
... WGS- analysis can be used to precisely refute an outbreak of S. maltophilia. Additionally, WGS- based typing docu- mented sanitary installations in HSCT units to be an actual source or result of transmission ... See full document
5
High Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing
... MST method permits the strains of the Copenhageni and Ictero- haemorrhagiae serovars to be differentiated into five distinct pro- files, in contrast to the unique profile by VNTR analysis (18). These two serovars are ... See full document
8
Multilocus Sequence Typing of Total Genome Sequenced Bacteria
... Illumina sequence data are assembled using Velvet, version 1.1.04 (34). Prior to assembly, paired-end data are filtered and trimmed using the following steps. (i) All reads containing the character N are removed. ... See full document
7
Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter suis
... MLST analysis of in vitro-isolated H. suis strains and H. suis strains present in porcine and human gastric tissue. Based on the complete gene sequences, as described above, internal gene fragments/variability regions ... See full document
7
Multilocus sequence typing breathes life into a microbial metagenome
... nucleotide sequence and trace back to cultivable bacteria which are absolute genetic matches this DNA, is testament to its resolving power as a typing ...strain typing methods based on DNA fragment ... See full document
9
The microbial ecology of Campylobacter jejuni in New Zealand within a spatial temporal framework : a thesis presented in partial fulfilment of requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatu, New Zealand
... to genome comparisons, developed along the lines of MLST, called whole genome MLST (wgMLST) but with the potential to include regions between genes if the adjacent loci are defined, has been developed [297] ... See full document
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