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[PDF] Top 20 HAPIscreen, a method for high-throughput aptamer identification

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HAPIscreen, a method for high-throughput aptamer identification

HAPIscreen, a method for high-throughput aptamer identification

... We then used such a strategy for evaluating the evolu- tion of populations derived from 7 rounds of RNA SELEX previously carried out in our laboratory against the domain II (DII) of the Hepatitis C Virus mRNA internal ... See full document

10

A High Throughput Approach for Identification of Nontuberculous Mycobacteria in Drinking Water Reveals Relationship between Water Age and Mycobacterium avium

A High Throughput Approach for Identification of Nontuberculous Mycobacteria in Drinking Water Reveals Relationship between Water Age and Mycobacterium avium

... as high as the extraction efficiencies achieved with the commercial kits ...extraction-independent method to verify the concentrations of cells used to spike samples limits the interpretation of their ...PC2 ... See full document

13

A high throughput method for identifying personalized tumor- associated antigens

A high throughput method for identifying personalized tumor- associated antigens

... the method of selection for antibody- binding peptides, which included an initial step of preadsorption of the phage libraries on normal immunoglobulins, prevented the identification of epitopes which are ... See full document

8

Identification of Long-Range Regulatory Elements in the Human Genome

Identification of Long-Range Regulatory Elements in the Human Genome

... using high-throughput sequencing (Chepelev et ...this method, their approach is different from unbiased approaches like Hi-C because it requires an antibody to a specific histone modification, ... See full document

141

A high-throughput amplicon-based method for estimating outcrossing rates

A high-throughput amplicon-based method for estimating outcrossing rates

... Sequencing quality will also depend on the heterogene- ity of sequences as it is used during the first amplification cycles for cluster identification and phasing/pre-phasing calibration [31]. Low-sequence ... See full document

14

MowJoe: a method for automated-high throughput dissected leaf phenotyping

MowJoe: a method for automated-high throughput dissected leaf phenotyping

... the identification of individual leaf- lets in the binarized ...our method, we manually measured the rachis and the petiolule of 5 leaves (Additional file 1: ... See full document

9

Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening

Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening

... Statistical significance of differences between fluores- cence intensities of drug-treated and untreated control wells was evaluated using Welch’s t-test followed by Holm’s step-down method for multiplicity ... See full document

11

High Throughput Identification of Clinical Pathogenic Fungi by Hybridization to an Oligonucleotide Microarray

High Throughput Identification of Clinical Pathogenic Fungi by Hybridization to an Oligonucleotide Microarray

... Our findings demonstrated that a series of species-specific hybridization profiles can be obtained by an oligonucleotide microarray method. This detection system can identify the 20 common pathogenic species C. ... See full document

7

A novel cost effective and high-throughput isolation and identification method for marine microalgae

A novel cost effective and high-throughput isolation and identification method for marine microalgae

... and high-throughput isolation and identifi- cation method for marine ...combined high throughput isolation by streaking cells from enrich- ment cultures on agar plates with subsequent ... See full document

10

High throughput molecular identification of Staphylococcus spp  isolated from a clean room facility in an environmental monitoring program

High throughput molecular identification of Staphylococcus spp isolated from a clean room facility in an environmental monitoring program

... a high-throughput and rapid identification method using both API Staph identifica- tion system followed by PCR analysis of the gap gene, for various Staphylococcus species isolated from envir- ... See full document

7

QsRNA seq: a method for high throughput profiling and quantifying small RNAs

QsRNA seq: a method for high throughput profiling and quantifying small RNAs

... PAGE-based method was established in ...PAGE-based method with three different barcodes and three technical replicas using QsRNA-seq with the same barcode ...relatively high, ranging from 20% to ... See full document

12

A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans

A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans

... of high numbers of animals, and are ...with high time reso- lution (Schnabel et ...and high-throughput method for continuous monitoring of development in ...The method is ... See full document

10

Efficient high throughput molecular method to detect Ehrlichia ruminantium in ticks

Efficient high throughput molecular method to detect Ehrlichia ruminantium in ticks

... The limit of detection of the pCS20 Sol1 TqM and Sol1 SG is three E. ruminantium copies per reaction, which is better than the pCS20 nested PCR (15 copies per reaction in this study, 6 copies in another study [18]), the ... See full document

12

Identification of microRNAs in PCV2 subclinically infected pigs by high throughput sequencing

Identification of microRNAs in PCV2 subclinically infected pigs by high throughput sequencing

... wasting syndrome, (PMWS) [5], an emerging disease in swine first described in 1991 [6]. PCV2 infection is wide- spread and its most frequent manifestation is by means of a subclinical infection. PCV2 is ubiquitous in ... See full document

7

A Method for Gas Identification in Thermal Dispersion Mass Flow Meters

A Method for Gas Identification in Thermal Dispersion Mass Flow Meters

... a method for correcting the effect of the coatings that are deposited on the thermal flow sensor, which can be carried out simultaneously with a measurement of the mass flow ...diagnostics method that is ... See full document

10

A high-throughput method for detection of DNA in chloroplasts using flow cytometry

A high-throughput method for detection of DNA in chloroplasts using flow cytometry

... DAPI is a DNA-binding fluorophore commonly used for fluorescence microscopy and flow cytometry [17-20]. In this study, we have assessed its utility for flow cytometric analysis of the DNA content of Arabidopsis ... See full document

13

Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and the formation of a nitrogen-fixing cyanosphere

Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and the formation of a nitrogen-fixing cyanosphere

... 0.95. High-quality sequences (length > 200 bp, minimum average Phred score 25) were further assigned to individual samples, and barcodes were removed using the Qiime ... See full document

12

Identification and genome analysis of tomato chlorotic spot virus and dsRNA viruses from coinfected vegetables in the Dominican Republic by high throughput sequencing

Identification and genome analysis of tomato chlorotic spot virus and dsRNA viruses from coinfected vegetables in the Dominican Republic by high throughput sequencing

... The Tomato chlorotic spot virus (TCSV) was first reported in the 1980s, having its occurrence limited to Brazil and Argentina. Due to an apparent mild severity in the past, molecular studies concerning TCSV were ... See full document

9

Original Article Identification of new susceptible genes associated with familial carotid body tumor of Chinese pedigree by high-throughput genome sequencing

Original Article Identification of new susceptible genes associated with familial carotid body tumor of Chinese pedigree by high-throughput genome sequencing

... Our current study presents results of high- throughput whole exome sequencing of a Chinese pedigree with HNPGL. We discovered some new susceptible genes, which never be reported. Among them, we find the ... See full document

9

Familial Hypercholesterolemia: From Diagnosis to Treatment

Familial Hypercholesterolemia: From Diagnosis to Treatment

... indicate high efficacy and safety for this option but According to the infrequency of liver donor and complexities of the transplant and post-transplant management this is not a popular choice for treatment ... See full document

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