[PDF] Top 20 Helicase-primase complex of herpes simplex virus type 1: a mutation in the UL52 subunit abolishes primase activity.
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Helicase-primase complex of herpes simplex virus type 1: a mutation in the UL52 subunit abolishes primase activity.
... Examination of the amino acid sequence of UL5 strongly suggests that it is the helicase of the complex, as it shares conserved sequence motifs with other known helicases 25, 29 and mutat[r] ... See full document
9
Mutations in the Putative Zinc-Binding Motif of UL52 Demonstrate a Complex Interdependence between the UL5 and UL52 Subunits of the Human Herpes Simplex Virus Type 1 Helicase/Primase Complex
... Herpes simplex virus type 1 (HSV-1) encodes a heterotrimeric helicase-primase (UL5/8/52) ...in helicase superfamily 1, and UL52 contains ... See full document
9
A Mutation in the Human Herpes Simplex Virus Type 1 UL52 Zinc Finger Motif Results in Defective Primase Activity but Can Recruit Viral Polymerase and Support Viral Replication Efficiently
... the UL52 subunit have been shown to drastically affect primase activity (4, 12, 18, 32), and mutations in the helicase motifs of UL5 were seen to abolish helicase activity ... See full document
10
Inhibition of Herpes Simplex Virus Replication by a 2-Amino Thiazole via Interactions with the Helicase Component of the UL5-UL8-UL52 Complex
... with virus- specific processes or incapacitating the host cell. If virus mu- tants resistant to an inhibitory compound can be selected, it is likely that the target of the inhibitor is a viral ...the ... See full document
9
Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... ssDNA-binding activity of UL9, a ssDNA- binding assay was ...S110T mutation, designed in a residue pre- dicted to be oriented toward the ATP-binding cleft and to not contact ssDNA, would exhibit little or ... See full document
12
The catalytic subunit of the DNA polymerase of herpes simplex virus type 1 interacts specifically with the C terminus of the UL8 component of the viral helicase-primase complex.
... for 1 h at ...for 1 h at ...diluted 1:400 in PBS containing 2% FCS was reacted for 1 h at ...diluted 1:500 in PBS containing 2% FCS and the chromogenic substrate 2,29- ... See full document
8
A C-Terminal Helicase Domain of the Human Papillomavirus E1 Protein Binds E2 and the DNA Polymerase α-Primase p68 Subunit
... catalytic activity, while the function of p68 is unclear (reviewed in reference ...The primase function resides in the p58 and p48 subunits; p48 has primase activity, while p58 may mediate or ... See full document
13
Baculovirus Replication Factor LEF-1 Is a DNA Primase
... the primase active site (3). The lef-1 gene of AcMNPV encodes a protein of 266 amino acids with a calculated molecular mass of 30,780 ...enzymatic activity. In herpes simplex ... See full document
11
Role of the Herpes Simplex Virus Helicase-Primase Complex during Adeno-Associated Virus DNA Replication
... other UL52 zinc finger mutant, CC3,4AA (1), exhibited re- duced levels of AAV DNA replication but retained the ability to mediate nuclear colocalization of ICP8 and Rep ...of UL52 to promote assembly ... See full document
10
Recruitment of Polymerase to Herpes Simplex Virus Type 1 Replication Foci in Cells Expressing Mutant Primase (UL52) Proteins
... the herpes simplex virus (HSV) type 1 replication apparatus leading to replication compartments likely involves the initial assembly of five viral replication proteins, ICP8, UL9, and ... See full document
11
The UL5 gene of herpes simplex virus type 1: isolation of a lacZ insertion mutant and association of the UL5 gene product with other members of the helicase-primase complex.
... In this study, we isolated a permissive cell line L2-5 which contains the wild-type UL5 gene under the control of the strong and inducible promoter for the large subunit of herpes simple[r] ... See full document
11
An Intertypic Herpes Simplex Virus Helicase-Primase Complex Associated with a Defect in Neurovirulence Has Reduced Primase Activity
... pheno- type of R13-1 is due to expression by this virus of an intertypic helicase-primase complex containing subunits of both HSV-1 and HSV-2 ...recombinant virus ... See full document
7
Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... Helicase-primase complex forms higher-order ...of complex bind to DNA in the presence of AMP-PNP. (iii) Primase activity assays reveal co- operative dependence on protein ... See full document
11
Different Regions of Primase Subunit p48 Control Mouse Polyomavirus and Simian Virus 40 DNA Replication In Vitro
... p48 primase. The murine p48 subunit controls the replication of PyV DNA in ...primate virus in vitro (4, ...(aa) 1 to 128, with the remaining amino acids being encoded by the human cDNA, ... See full document
10
An autophosphorylating but not transphosphorylating activity is associated with the unique N terminus of the herpes simplex virus type 1 ribonucleotide reductase large subunit.
... 7511-7516 0022-538X/92/127511-06$02.00/0 Copyright C 1992, American Society for Microbiology An Autophosphorylating but Not Transphosphorylating Activity Is Associated with the Unique N [r] ... See full document
6
Mechanisms of herpes simplex virus type 1 reactivation.
... collagenase type XI (1 mg/ml; Sigma) and collagenase type IV (1 mg/ml; Sigma) and incubated at 37 8 C for 75 to 90 ...a 1-ml serological ... See full document
10
A Mutation in the UL24 Gene Abolishes Expression of the Newly Identified UL24.5 Protein of Herpes Simplex Virus 1 and Leads to an Increase in Pathogenicity in Mice
... substitution mutation that blocked expression of ...mice 1 to 3 ...some herpes- viruses full-length UL24 itself is responsible for the functions dedicated to ... See full document
17
Protein kinase activity associated with the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10).
... The second line of evidence supporting our conclusion that the PK activity is encoded by the HSV-2 RR1 gene is the finding that both ICP10 and a 57-kDa protein precipitated from cells tr[r] ... See full document
10
Conditional mutations in the yeast DNA primase genes affect different aspects of DNA metabolism and interactions in the DNA polymerase alpha-primase complex.
... Different pril and pri2 conditional mutants of Saccharomyces cereuisiae altered, respectively, in the small (p48) and large (p58) subunits of DNA primase, show an en[r] ... See full document
9
A Null Mutation in the Gene Encoding the Herpes Simplex Virus Type 1 UL37 Polypeptide Abrogates Virus Maturation
... the herpes simplex virus type 1 (HSV-1) ...null mutation was generated in the gene encoding this ...mutant virus, transformed cell lines that express the UL37 gene ... See full document
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