[PDF] Top 20 The Herpes Simplex Virus 2 UL21 Protein Is Essential for Virus Propagation

... infectious virus in noncomplementing cells, whereas the repaired HSV-2 ⌬UL21 strain grew to wild-type (WT) titers, indicating that UL21 is essential for virus ...a 2-h ... See full document

... conserved protein in the tegument of alphaherpesviruses and has multiple important albeit poorly understood func- tions in viral replication and ...of UL21, we determined the crystal structure of its ... See full document

... of virus replication kinetics. P1 virus stocks were used at both low ...Total virus (cells and supernatant) was collected at 0, 6, 12, 18, 24, 36, and 48 h postinfection, and cellular virions were ... See full document

... 65-kDa protein that we found first in pull-down experiments using GST-UL11 (17) has proven to be the UL21 tegument ...that UL21 does not interact with UL11 but instead binds to it indirectly through ... See full document

... The finding that the PRV UL21 gene is not essential for virus viability indicates that the PRV UL21-encoded protein has a regulatory or accessory role in processing of viral DNA, in pack[r] ... See full document

... Consistent with their ability to induce expression of early polypeptides, all of the mutants induced the synthesis of substantial levels of viral DNA at the nonpermissive temperature.. T[r] ... See full document

... The 65-kilodalton DNA-binding protein (65KDBP) of herpes simplex virus type 1 (HSV-1), the product of the UL42 gene, is required for DNA replication both in vitro and in vivo, yet its ac[r] ... See full document

... 16 protein from HSV-2 have shown that this protein may possess DNA bind- ing activity ...viral protein synthesis by promoting the incorpo- ration of U L 16 into ... See full document

... To further understand the relationship between the structure of UL42 and the role that it plays during virus replication, we analyzed an extensive set of mutant UL42 proteins for the abi[r] ... See full document

... Comparison of the predicted structure of the HSV-2 ICP32/VP19c protein with the structures of related proteins encoded by other herpesviruses suggested that the internal capsid architect[r] ... See full document

... many essential processes in the viral replication cycle, yet few have been characterized in ...detail. UL21 is one such multifunctional tegument protein and is conserved among alphaherpes- ...While ... See full document

... the herpes simplex virus 1 U L 31 and U L 34 proteins interact and form a complex at the inner nuclear membranes of infected cells, where both play important roles in the envelop- ment of ... See full document

... DISCUSSION As presented in this paper, HSV-1 and HSV-2 possess cor- responding sets of 74 genes that encode distinct proteins. From the characteristics of the genomic sequences, from the com- parative analyses ... See full document

... viral protein which cross-reacts non-specifically with the antibody, we immunostained blots produced from gels of viral proteins from our HSV1 ...kDa protein in uninfected cells, and also, at a higher ... See full document

... viral protein which cross-reacts non-specifically with the antibody, we immunostained blots produced from gels of viral proteins from our HSV1 ...kDa protein in uninfected cells, and also, at a higher ... See full document

... insert 2), as well as by extra sequences at the N terminus ...a protein sequence for functional do- ...target protein or specific assays to measure particular properties, it is usually not possible ... See full document

... The herpes simplex virus protein UL25 attaches to the external vertices of herpes simplex virus type 1 capsids and is required for the stable packaging of viral ...the ... See full document

... binant herpes simplex virus type 1 expressing a green fluorescent protein (GFP)-tagged tegument protein, namely VP22, to show that VP22 is localized exclusively to the cytoplasm during ... See full document

... DISCUSSION The phenotype of all UL28-null and UL28-temperature- sensitive mutants is a cessation of viral replication at the point of DNA cleavage and encapsidation (1, 6, 14, 17, 23). There- fore, the presumed function ... See full document

... It is our prediction that the ability of HSV-2 to kill T cells plays a role in mitigating the cell-mediated immune response in vivo. In the present study, we have begun to elucidate the mechanism behind apoptosis ... See full document