[PDF] Top 20 Human VAP-B Is Involved in Hepatitis C Virus Replication through Interaction with NS5A and NS5B

... called VAP-33 ...proteins involved in HCV RNA replication (19, 49, ...of VAP-A resulted in relocation of NS5B from detergent-resistant to detergent-sensitive mem- branes and reduced HCV ... See full document

... with NS5B, enhances NS5B-dependent RdRp ...HCV replication, suggesting that both FASN activity and protein expression are involved in the regulation of HCV ...with NS5B, into the ... See full document

... factors involved in HCV replication, we examined the effects on viral RNA replication of transfec- tion of SGR-N cells with siRNAs against genes encoding nine proteins belonging to diverse classes of ... See full document

... the interaction of PREB and caveolin with the DRM structure, as observed for other raft-localized pro- teins (28), suggesting that PREB was present in DRM in the cells cotransfected with ...the interaction ... See full document

... Hsp90 through the FxxW motif in the N-terminal p23 homology do- main, and the interaction of hB-ind1 with Hsp90 was shown to be further intensified by the expression of FKBP8, suggesting that FKBP8 and ... See full document

... viruses, hepatitis C virus (HCV) replicates its genome in a remodeled intra- cellular membranous structure known as the membranous web ...formation through interaction with the ... See full document

... Hepatitis C virus (HCV) is a major causative agent of chronic hepatitis, liver cirrhosis, and hepatocellular ...NS4B, NS5A, and NS5B) of HCV were associated with the endoplasmic ... See full document

... The NS5A protein has been shown to interact with several host proteins, including vesicle-associated membrane protein (VAMP)-associated protein (VAP) subtype A (VAP-A) (44) and subtype B ... See full document

... protein-protein interaction be- tween Plk1 and ...with NS5A in the replicon ...and NS5A-HA or GST-HA-expressing vectors and used them for coimmuno- precipitation ...of NS5A- HA, the anti-HA ... See full document

... buffer B (20 mM Tris [pH ...filtration through a Whatman filter paper, loaded onto a ...buffer B, the RPA-containing fractions were eluted with 2 ml of buffer C (20 mM Tris [pH ... See full document

... HCV NS5A. We next investigated how MxB interferes with the formation of NS5A-CypA ...to NS5A and prevents the binding of CypA to ...that NS5A was coprecipitated with MxB as well as with the ... See full document

... of replication-defective NS5A ...three NS5A complementation groups (groups A, B, and C [Table 1]), each of which is potentially associated with a distinct RNA replication ... See full document

... as human immunodeficiency virus and ...RNA replication in vitro ...the interaction between NS5B and cyclophilin B, which is required for an efficient RNA-binding of NS5B ... See full document

... disrupted interaction with these cellular factors but failed to hinder HCV RNA ...larger interaction surfaces in domain III, including p53 and human TATA box-binding protein-associated factor II, ... See full document

... full-length NS5B, we utilized NS5Bt (68), a soluble form of NS5B in which the C-terminal 21 aa were truncated, to dissect the interaction between NS5B and nucleo- ...with NS5B in ... See full document

... the replication phenotype to resemble that of the pa- rental replicon ...and C 9302 all generated significant numbers of G418- resistant colonies after transduction and ...loop interaction with the 3 ... See full document

... sequences occupying the bulge loop of SL9266 are highly conserved, whereas those forming the upper and lower duplexes show more variability. This accounts for the different level of conservation of base-pairing between ... See full document

... of hepatitis C virus (HCV) on the antigenic properties of the NS5 protein was studied by using recombinant ...HCV NS5A protein at amino acids 2212 to ...the NS5A immunodominant ... See full document

... and NS5A hyperphosphorylation of mutant NS4A ...the replication defects of our mutant proteins could simply be due to defective polyprotein ...vaccinia virus that expresses T7 RNA polymerase, and ... See full document

... SDS-PAGE/Western blotting. Cells were washed twice in PBS, lysed in 1⫻ Glasgow lysis buffer (GLB; 1% [vol/vol] Triton X-100, 120 mM KCl, 30 mM NaCl, 5 mM MgCl 2 , 10% [vol/vol] glycerol, 10 mM PIPES [piperazine-N,N = ... See full document