[PDF] Top 20 Direct Identification of Pathogens in Urine by Use of a Specific Matrix Assisted Laser Desorption Ionization–Time of Flight Spectrum Database
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Direct Identification of Pathogens in Urine by Use of a Specific Matrix Assisted Laser Desorption Ionization–Time of Flight Spectrum Database
... Optimized direct identification protocol for bacterial identification. Each urine sample (1 ml) was vigorously mixed with 300 l of a 5% saponin solution (Sigma-Aldrich, Saint-Quentin Fallavier, France) and ... See full document
9
Direct Identification of Urinary Tract Pathogens from Urine Samples by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification ... See full document
6
Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... The use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative ...addition, matrix-assisted laser ... See full document
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Evaluation of a Simple Protein Extraction Method for Species Identification of Clinically Relevant Staphylococci by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... a database for Biotyper and has been recommended by the manufacturer as a reference method for identifications (1, 4, ...the identification rate of the standard extraction method was ...routine use ... See full document
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Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
... This study was approved by the Committee for Clinical Scientific Research of Tohoku Medical and Pharmaceuti- cal University Hospital on July 08 and December 10, 2015 (Nos. ID2015-2-008 and ID2015-2-037, respectively), ... See full document
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Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... of pathogens. The morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in ...However, matrix-assisted ... See full document
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Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) also known as Bruker MALDI Biotyper is a technology that allows instant ... See full document
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The Genus Corynebacterium and Other Medically Relevant Coryneform Like Bacteria
... (iii) Identification using the MALDI-TOF approach. Matrix- assisted laser desorption ionization–time of flight (MALDI-TOF) analysis is rapidly becoming a routinely ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... the identification of pathogens from blood cultures, these will vary because of a number of other factors, such as the exact distribution of typical pathogens isolated, the utilization of MALDI-TOF ... See full document
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Evaluation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates
... for spectrum analysis and com- parison with the database (updated with 5,627 species plus the specific Mycobacteria Library ...turnaround time until MALDI-TOF identification was ob- ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii
... the use of an average statistical algorithm as implemented in the BioTyper ...the database did not ...MALDI spectrum matching the expected one in the reference database (Table ... See full document
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Evaluation of the Andromas Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram Positive Bacilli
... MS identification system has its own spec- ificities, including the hardware itself (the mass spectrometer), the way the database was built, and the algorithms used to compare the mass spectrum of a ... See full document
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Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... potential pathogens that are isolated from stool ...pathogen identification itself, the limitations of MALDI-TOF MS must be ...the identification of Shigella or ...MS database to differentiate ... See full document
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Accuracy of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Clinical Pathogenic Fungi: a Meta Analysis
... the database by establishing reference spectra for clinical fungi, while others ...the identification of yeasts, and 8 focused on mold ...the identification of ... See full document
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Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... a laser frequency of 60 Hz across a mass/ charge ratio (m/z) of 2,000 to 20,000 Da using the MicroFlex LT mass spectrometer (Bruker ...each spectrum, 240 laser shots in 40-shot steps from different ... See full document
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Microbial Typing by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?
... strain identification/epidemiological typing tool, especially since the data are available in most cases at no additional cost: the typing information, or at least part of it, is contained within the primary ... See full document
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Identification of Rare Pathogenic Bacteria in a Clinical Microbiology Laboratory: Impact of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... mass spectrum database. The Brüker database up- dated with a laboratory collection of spectra from clinical isolates identi- fied by 16S rRNA gene sequencing was used from 1 September 2009 to 31 ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... MS identification is based on obtaining and comparing the unique protein profile of an isolate to a reference library using spectral pattern ...i.e., identification is limited only by the size of the ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... MALDI matrix (a saturated solution of ␣ -cyano-4-hydroxy-cinnamic acid in 50% ...typical spectrum, derived from ...a spectrum for identification ...grated database provided by the ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors
... rapid identification of tick vectors. This method was used for the first time to identify ticks removed from ...the time required for molecular methods, and the completion of this assay does not ... See full document
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