[PDF] Top 20 Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... of protein clearly affects cooperative binding of the ...the protein involved in this impor- tant ...This region of ICP8 contains one cysteine residue at position ...this ... See full document
9
The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... different region, namely, between residues 386 and 902 ...the single ICP8 zinc atom is most likely ...the protein without being directly involved in contacts with nucleotides ...mediate ... See full document
11
Molecular Genetic and Biochemical Characterization of the Vaccinia Virus I3 Protein, the Replicative Single-Stranded DNA Binding Protein
... I3 protein, to no ...the herpes simplex virus (HSV)-encoded SSB (ICP8), a region containing a similar set of ß-sheets and a motif of aromatic and basic residues was identified in the ... See full document
13
Characterization of a herpes simplex virus sequence which binds a cellular protein as either a single-stranded or double-stranded DNA or RNA.
... 3436 N[GT/UGGGT/UGGG]2N c 10 with the nine-1 relative to the major OrisRNA; ii as measured in competition assays, the proteins involved in recognition and binding of the single-stranded [r] ... See full document
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... minimal protein-to-protein variation in staining, allowing quantitative comparison between proteins ...broad protein band with a molecular weight (MW) of about 80,000 was observed in all the capsid ... See full document
9
Characterization of the Single-Stranded DNA Binding Protein Encoded by the Vaccinia Virus I3 Gene
... Partial protein sequences from various SSBs were aligned with that portion of the gene 5 protein (fd bacteriophage) involved in DNA binding (modified from references 11, 34, and ...a ... See full document
10
Relationship between TATA-binding protein and herpes simplex virus type 1 ICP4 DNA-binding sites in complex formation and repression of transcription.
... the cooperative nature of complex formation, we infer that there are protein-protein contacts between ICP4 and the DB complex on ...the binding of ICP4 to DNA is transmitted down the ... See full document
8
Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... of herpes simplex virus type I (HSV-1), consisting of UL5, UL8, and UL52, possesses 5 ⴕ to 3 ⴕ helicase, single-stranded DNA (ssDNA)-dependent ATPase, primase, and DNA ... See full document
11
Identification of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... 818 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Hybrid arrest of in vitro translation was used to localize the region of the herpes simplex virus type 1 genome enco[r] ... See full document
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The major herpes simplex virus DNA-binding protein holds single-stranded DNA in an extended configuration.
... Measurements of single-stranded fd DNA molecules saturated with protein and spread for electron microscopy by using both the aqueous and formamide spreading techniques indicated that the[r] ... See full document
6
Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... varicella virus); E, equine; G, gallid; M, meleagrid (turkey); MDV, Marek’s disease virus; PRV, pseudorabies virus; VZV, varicella-zoster ...The single mutations generated (slashes) and the ... See full document
12
Surface lysine and tyrosine residues are required for interaction of the major herpes simplex virus type 1 DNA-binding protein with single-stranded DNA.
... 11 0022-538X/92/116273-07$02.00/0 Copyright © 1992, American Society for Microbiology Surface Lysine and Tyrosine Residues Are Required for Interaction of the Major Herpes Simplex Virus [r] ... See full document
7
Genetic and biochemical identification of a novel single stranded DNA binding complex in Haloferax volcanii
... Genomic DNA must be unwound in order to be replicated or repaired, leaving it vulnerable to nuclease and chemical attack as well as open to the possibility of forming secondary ...structures. Binding of the ... See full document
14
Localization of the gD-Binding Region of the Human Herpes Simplex Virus Receptor, HveA
... Localization of the CW MAb epitopes. To further character- ize the CW MAbs, we examined their binding to the four HveA truncations (described earlier) by Western blot analysis (Fig. 7). CW1, CW2, and CW4 reacted ... See full document
10
Characterization of two conformational forms of the major DNA-binding protein encoded by herpes simplex virus 1.
... SPANG Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115 We have resolved two electrophoretic species of the major DNA-binding protein[r] ... See full document
6
DNA Binding Activity of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, Can Be Modulated by Sequences in the N Terminus: Correlation between Transdominance and DNA Binding
... stable protein, cells were transfected and cell lysates were analyzed by Western blotting as described in Materials and ...UL9 protein was detected using the 17B monoclonal antibody, which was directed ... See full document
10
The lef-3 gene of Autographa californica nuclear polyhedrosis virus encodes a single-stranded DNA-binding protein.
... Defective virus particles of AcNPV were generated by serial undiluted passage in insect ...3-kb DNA fragment corresponding to the HindIII-K region of the viral ...This region of the AcNPV ... See full document
5
Activities of heterodimers composed of DNA-binding- and transactivation-deficient subunits of the herpes simplex virus regulatory protein ICP4.
... Despite the transcriptional defect of the vil2 and vil3 polypeptides observed in infected Vero cells, transcription of the gene for tk was induced upon infection of X25 cells with either[r] ... See full document
9
Phylogenetic and Functional Analysis of the Bacteriophage P1 Single-Stranded DNA-Binding Protein
... a single-stranded DNA-binding protein (SSB-P1), which shows 66% amino acid sequence identity to the SSB protein of the host bacterium Escherichia ... See full document
7
The baculovirus single-stranded DNA binding protein, LEF-3, forms a homotrimer in solution.
... polyhedrosis virus required for transient DNA replication and has the properties of a single-stranded DNA binding ...transient DNA replication, suggesting that this ... See full document
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