[PDF] Top 20 Identification of stable reference genes for quantitative PCR in koalas
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Identification of stable reference genes for quantitative PCR in koalas
... identifying genes involved in disease ...transcription quantitative polymerase chain reaction (RT-qPCR) is the gold standard procedure for quan- tifying gene ...in quantitative data analysis due to ... See full document
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Identification of stable reference genes for quantitative PCR in koalas
... Supplementary Table S3: Overall ranking of candidate reference genes analysed separately in Queensland, South Australia and both populations combined Ranking order Method.. Recommended c[r] ... See full document
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Microarray based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress
... The identification of suitable reference genes can be ...“model” genes to be used in data normalisation. Usually the allocated genes vary with the plant species, as well with the ... See full document
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Exploring Valid Reference Genes for Quantitative Real-time PCR Analysis in Plutella xylostella (Lepidoptera: Plutellidae)
... appropriate reference genes with stable expression across various biotic and abiotic ...didate genes from ...housekeeping genes [ACTB, GAPDH, 18S rRNA, RPL32, RPS13, EF1, ribosomal ... See full document
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<p>Identification and Validation of Reference Genes Selection in Ovarian Cancer Exposed to Hypoxia</p>
... the quantitative real-time PCR experiments to a stable reference gene, which is not affected by the experimental ...internal reference genes were less than ...most stable ... See full document
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Validation of reference genes for expression analysis by quantitative real time PCR in Leptinotarsa decemlineata (Say)
... and PCR process. The use of HKGs as refer- ence genes to normalize gene expression is a strategy to minimize the variations of ...good reference gene should meet three ...is stable in all test ... See full document
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... internal reference genes to deter error such as inaccurate quantification of RNA, the quality of the RNA, and difference during cDNA synthesis that can trigger variations in PCR ...internal ... See full document
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Selection of reliable reference genes for quantitative real time PCR in human T cells and neutrophils
... instable reference genes correctly indicated an increase in IL-2 transcripts, which may be sufficient if only an on-/off response is to be ...inappropriate reference genes leads to unreliable ... See full document
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Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis
... candidate reference gene analysis were designed via a combinatorial approach using the Primer3 ...candidate reference genes were tested using a standard RT-PCR approach and the construction of ... See full document
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Selection of reference genes for normalization of quantitative real time PCR in organ culture of the rat and rabbit intervertebral disc
... ence genes over the organ culture of the rat and rabbit IVD was analyzed using the geNorm software applica- ...four genes (GAPDH, CycA, Hprt1, and 18S rRNA) in the rat IVD reached a high expression ... See full document
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Selection and validation of reference genes for quantitative gene expression studies by real time PCR in eggplant (Solanum melongena L)
... real-time PCR approach as the most appropriate strat- egy ...real-time PCR based approach can be dependable only when the results are ...unreliable reference genes or internal control ... See full document
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Reference gene validation for qPCR in rat carotid body during postnatal development
... of reference genes for mRNA normalization in quantitative real-time PCR must be validated for a given tissue and set of ...affect reference gene expression. In order to determine the ... See full document
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Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR
... transcriptionally stable reference gene during olive fruit development, followed by GAPDH2, while GAPDH1 showed the widest variation and was considered the least stable ...most stable in ... See full document
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Reference gene selection for quantitative PCR studies in bovine neutrophils
... trophil reference genes was evaluated using generally accepted Excel-based software tools [24] according to instructions provided by the program developers, ...neutrophil reference genes was ... See full document
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Selection of reference genes for quantitative real time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera
... six genes were selected from a 454 cDNA library of Boechera: RPS18 (Ribosomal sub protein 18), Efalpha1 (Elongation factor 1 alpha), ACT 2 (Actin2), UBQ (polyubiquitin), PEX4 (Peroxisomal ubiquitin conjugating ... See full document
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Validation of reference genes for quantitative real time PCR studies in the dentate gyrus after experimental febrile seizures
... candidate reference genes determined by geNorm was compared with Microsoft Excel-based applet termed ...candidate reference gene and ranks the genes according to their expression stability in ... See full document
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Reference genes for reverse transcription quantitative PCR in canine brain tissue
... candidate reference genes evaluated in the present study were included in the research of Park et ...most stable control gene for whole brain ...the genes with relatively moderate ... See full document
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Identification and validation of quantitative PCR reference genes suitable for normalising expression in normal and dystrophic cell culture models of myogenesis
... Total RNA was isolated by addition of 100ul TRIzol (Fisher Scientific) to each well of cells, agitation with a cell scraper and transfer to a clean microcentrifuge tube. Phase-separation was initiated by addition of 30ul ... See full document
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Identification and validation of Aeluropus littoralis reference genes for Quantitative Real-Time PCR Normalization
... rDNA genes including ...rDNA genes that can be arranged in tandem repeating units (placed side by side) ...these genes is the generation of ribosomal subunits required for protein ...rDNA ... See full document
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Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
... Quantitative PCR has become the gold standard for the quantitative analysis of nucleic acids because of its high sensitivity, adequate reproducibility, broad quantifi- cation range and ease of ... See full document
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