[PDF] Top 20 An immunoassay for the study of DNA-binding activities of herpes simplex virus protein ICP8.
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An immunoassay for the study of DNA-binding activities of herpes simplex virus protein ICP8.
... Vero cells 731 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest An immunoassay was used to examine the interaction between a herpes simplex virus protein, ICP8, and vari[r] ... See full document
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A cytomegalovirus protein with properties of herpes simplex virus ICP8: partial purification of the polypeptide and map position of the gene.
... 3143 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We demonstrated the presence of a single-stranded DNA-binding protein in human cytomegalovirus CMV-infected cells w[r] ... See full document
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... The herpes simplex virus type 1 (HSV-1) UL29 gene prod- uct, commonly designated ICP8, is the major single-strand- DNA-binding protein (SSB) present in infected ...cells. ... See full document
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Location, transcript analysis, and partial nucleotide sequence of the cytomegalovirus gene encoding an early DNA-binding protein with similarities to ICP8 of herpes simplex virus type 1.
... 1364 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The results presented here locate the gene encoding an early, nonvirion, single-stranded DNA-binding protein of hum[r] ... See full document
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N-ethylmaleimide inhibition of the DNA-binding activity of the herpes simplex virus type 1 major DNA-binding protein.
... To determine whether this was indeed the case, equilibrium dialysis experiments with ICP8 and [32P]dT1o were done in the presence and absence of NEM as described DISCUSSION The results p[r] ... See full document
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A second-site revertant of a defective herpes simplex virus ICP4 protein with restored regulatory activities and impaired DNA-binding properties.
... ICP4 has been shown to specifically interact with lower-affinity sites that differ significantly from the consensus sequence found in the vicinity of early and late gene promoters 23, 25[r] ... See full document
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Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... Western blot analysis. Western blot analysis was performed as previously described (4). Anti-UL9 primary antibodies recognizing the C-terminal residues 841 to 851 (R250, a kind gift from M. Challberg, National Institutes ... See full document
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Intragenic complementation of herpes simplex virus ICP8 DNA-binding protein mutants.
... This paper reports that the carboxyl-terminal 28 residues of ICP8 are required for a functions involved in viral DNA replication and that the functions missing in nllSV can be supplied i[r] ... See full document
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Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... UL25 protein consists of numerous flexible loops extend- ing from a rigid core composed of several tightly packed alpha ...the protein and hence inhibit its ...this study contained insertions in the ... See full document
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Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... The herpes simplex virus type 1 (HSV-1) origin binding protein (OBP), the product of the UL9 gene, is one of seven HSV-encoded proteins required for viral DNA ...a DNA ... See full document
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Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein.
... 727 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The interaction of the herpes simplex virus type 1 HSV-1 major DNA-binding protein, infected-cell polypeptide 8 ICP8[r] ... See full document
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Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... to DNA in the presence of ...on protein concentration while ATPase and helicase activities do ...as protein denaturation at low concentra- tions or dissociation of the UL5-UL8-UL52 complex, ... See full document
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The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... for ICP8 was obtained from plasmid pE29 (66) and cloned into the pFastBacHTa (Gibco BRL) baculovirus vector in two ...wild-type ICP8 with a hexahistidine tag linked to the N terminus via a TEV protease ... See full document
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DNA-Binding Activity of Adeno-Associated Virus Rep Is Required for Inverted Terminal Repeat-Dependent Complex Formation with Herpes Simplex Virus ICP8
... precluding DNA helicase activity in vitro (3, 4, 35) and AAV DNA replication in vivo (2, ...with ICP8 and AAV ssDNA in vitro ...the DNA-binding domain that mediates site-specific bind- ... See full document
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document
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Genetic evidence for multiple nuclear functions of the herpes simplex virus ICP8 DNA-binding protein.
... 5258 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have isolated several mutant herpes simplex viruses, specifically mutated in the infected cell protein 8 ICP8 ge[r] ... See full document
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Correct intranuclear localization of herpes simplex virus DNA polymerase requires the viral ICP8 DNA-binding protein.
... In the absence of viral DNA replication, HSV Pol colocalized with the HSV DNA-binding protein ICP8 in nuclear framework-associated structures called prereplicative sites.. In the presenc[r] ... See full document
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Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1.
... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document
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In vitro characterization of a thermolabile herpes simplex virus DNA-binding protein.
... 31 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The major herpes simplex virus DNA-binding protein, ICP8, was purified from cells infected with the herpes simplex vi[r] ... See full document
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Renaturation of complementary DNA strands by herpes simplex virus type 1 ICP8.
... 6945 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complem[r] ... See full document
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