[PDF] Top 20 Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase.
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Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase.
... For the experiments in which the fate of the RNA primer was determined, the T348 fragment was excised from the single-stranded vector with EcoRI as previously described 5; the resulting [r] ... See full document
7
Specific Recognition and Cleavage of the Plus-Strand Primer by Reverse Transcriptase
... possess DNA polymerase and RNase H activities. During reverse transcription these activities are necessary for the pro- grammed sequence of events that include template switching and primer ... See full document
13
Mutations Proximal to the Minor Groove-Binding Track of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Differentially Affect Utilization of RNA versus DNA as Template
... on DNA templates ...on RNA templates in that the polymerization stopped after the addition of a single ...length DNA similar to the wild type when the rebinding of RT is allowed (nonprocessive ... See full document
9
Requirements for Minus-Strand Transfer Catalyzed by Rous Sarcoma Virus Reverse Transcriptase
... specific minus-strand transfer reactions that occur after the synthesis of minus strong-stop DNA and nonspecific strand switching on homopolymeric poly(rA) templates with ... See full document
7
Effects of Dipeptide Insertions between Codons 69 and 70 of Human Immunodeficiency Virus Type 1 Reverse Transcriptase on Primer Unblocking, Deoxynucleoside Triphosphate Inhibition, and DNA Chain Elongation
... Human immunodeficiency virus type 1 (HIV-1) reverse tran- scriptase (RT) is responsible for replication of the HIV-1 ge- nome and is therefore an important target ... See full document
7
Template Dimerization Promotes an Acceptor Invasion-Induced Transfer Mechanism during Human Immunodeficiency Virus Type 1 Minus-Strand Synthesis
... both RNA strands and proceeds through an initial docking of the acceptor on the nascent DNA followed by strand invasion ...the primer terminus, or the Lock step, occurred predominantly within ... See full document
12
Human immunodeficiency virus type 1 nucleocapsid protein promotes efficient strand transfer and specific viral DNA synthesis by inhibiting TAR-dependent self-priming from minus-strand strong-stop DNA.
... on synthesis of SP DNAs and efficiency of strand ...from reverse transcription of TAR in the donor RNA, is ultimately responsible for synthesis of SP prod- ...the synthesis of SP ... See full document
11
Effects of 3'-deoxynucleoside 5'-triphosphate concentrations on chain termination by nucleoside analogs during human immunodeficiency virus type 1 reverse transcription of minus-strand strong-stop DNA.
... or 1 m M) were more difficult to quantitate because of reduced processivity of RT as shown ...the RNA template (data not ...to 1/10 that of dNTP, preferential chain termina- tion by ddAMP was ... See full document
9
Mutations in the U5 Region Adjacent to the Primer Binding Site Affect tRNA Cleavage by Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo
... the RNA template strand near the scissile phosphate include the side chains of R448, N474, Q475, and H539 of p66 ...the RNA template 5 ⬘ of the scissile ...the RNA strand), it is not ... See full document
9
Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site.
... on synthesis of ( 2 ...the primer-template complex as well as synthesis of ( 2 )ssDNA ...nascent reverse-transcribed DNA products from infected cells by a quantitative PCR revealed no ... See full document
9
Effects of zidovudine-selected human immunodeficiency virus type 1 reverse transcriptase amino acid substitutions on processive DNA synthesis and viral replication.
... the RNA template (20, 27, ...increased DNA syn- thesis by this mutant during a single processive ...growing DNA primer strand before polymerase dissociation, may be greater than that of ... See full document
8
Human Immunodeficiency Virus Type 1 Central DNA Flap: Dynamic Terminal Product of Plus-Strand Displacement DNA Synthesis Catalyzed by Reverse Transcriptase Assisted by Nucleocapsid Protein
... the reverse transcriptase (RT)-associated RNase H (37, 38, ...3⬘PPT primer, and the other one, located at the center of the RNA chain, is referred to as the cPPT ...two RNA sequences ... See full document
13
Cross-Linking of the Fingers Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase to Template-Primer
... teins. Site-directed mutagenesis can be used to introduce cys- teines at specific sites in the protein; the cystines can then be modified with cross-linking agents. This approach has been used to study the interactions ... See full document
11
In Vitro Analysis of Human Immunodeficiency Virus Type 1 Minus-Strand Strong-Stop DNA Synthesis and Genomic RNA Processing
... the RNA annealed to the 26-base DNA primer, approximately 17 and 8 bases from the 3⬘ end of the DNA primer ...the primer was ...template RNA was digested in the region ... See full document
15
Hypersusceptibility to Substrate Analogs Conferred by Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... viral RNA to double-stranded DNA by re- verse transcriptase (RT) is a defining step in the retroviral life cycle (8) and a key target of therapy for human immunodefi- ciency virus ... See full document
10
Mutations in the Primer Grip of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Impair Proviral DNA Synthesis and Virion Maturation
... alternative primer pairs for determination of early and late steps of reverse ...each primer pair relative to the viral genome is schematically represented on the left of each ...The primer ... See full document
5
Reverse Transcriptase Inhibitors Can Selectively Block the Synthesis of Differently Sized Viral DNA Transcripts in Cells Acutely Infected with Human Immunodeficiency Virus Type 1
... of human immunodeficiency virus type 1 (HIV-1) reverse transcription by inhibitors of reverse transcriptase (RT) occurred most efficiently when the expected ... See full document
8
Actinomycin D Inhibits Human Immunodeficiency Virus Type 1 Minus-Strand Transfer in In Vitro and Endogenous Reverse Transcriptase Assays
... on human immunodeficiency virus type 1 (HIV-1) reverse transcription during the course of studies with a reconstituted system (re- ferred to here and in reference 28 as an ... See full document
9
The M184V Mutation in the Reverse Transcriptase of Human Immunodeficiency Virus Type 1 Impairs Rescue of Chain-Terminated DNA Synthesis
... the reverse reaction, ...chain-terminated DNA synthesis can be ...3TC-terminated primer us- ing wt HIV-1 RT and ...lished DNA-DNA primer-template system that was ... See full document
7
Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... Mutant mt4 particles produced at the nonpermissive tem- perature contain little or no detectable p66-p51 heterodimeric RT protein. This property is very similar to that described for an RT variant that emerged following ... See full document
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