[PDF] Top 20 Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.
Has 10000 "Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase." found on our website. Below are the top 20 most common "Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.".
Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.
... AUSTIN2 Laboratory of Molecular Microbiology' and Laboratory of Immunopathology,' National Institute of Allergy and Infectious Diseases, and Developmental and Metabolic Neurology Branch,[r] ... See full document
5
Mutations of the RNase H C Helix of the Moloney Murine Leukemia Virus Reverse Transcriptase Reveal Defects in Polypurine Tract Recognition
... and RNase H func- tions in ...reduced RNase H activity and the ability of the DNA polymerase to make long DNA, which accounted for their failure to ...less viral DNA than did WT ... See full document
14
Replication Defect of Moloney Murine Leukemia Virus with a Mutant Reverse Transcriptase That Can Incorporate Ribonucleotides and Deoxyribonucleotides
... which reverse transcription was blocked, virions were purified and incubated in vitro with nucleotides to mon- itor the synthesis of reverse transcription intermediates on the viral DNA template (the ... See full document
7
Mutations in the RNase H Primer Grip Domain of Murine Leukemia Virus Reverse Transcriptase Decrease Efficiency and Accuracy of Plus-Strand DNA Transfer
... The RNase H primer grip of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) contacts the DNA primer strand and positions the template strand near the RNase ... See full document
9
Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain
... N-terminal region of ␣ -helix B contains R585 and Y586 that may interact with the RNA and DNA strands, respec- ...just 3 ⬘ of DNA phosphate ( ⫺ ...nonreplicative virus with the mutant RT having only ... See full document
11
Reversion of a Moloney murine leukemia virus RNase H mutant at a second site restores enzyme function and infectivity.
... the virus, we subcloned this region from the cloned fragment of the revertant viral DNA back into pNCA, a plasmid containing a cloned copy of wild-type M- MuLV proviral DNA ...ers 3 9 Int and ... See full document
5
Specific Cleavages by RNase H Facilitate Initiation of Plus-Strand RNA Synthesis by Moloney Murine Leukemia Virus
... a single internal site independent of positioning by the RNA 5⬘ ...(Fig. 3, lanes 6 to 8; data not shown for 5⬘ end-labeled substrate), and no significant internal ...FIG. 3. RNase H ... See full document
11
RNase H Activity Is Required for High-Frequency Repeat Deletion during Moloney Murine Leukemia Virus Replication
... and RNase H activities of reverse transcriptase (RT) ...The RNase H activity, which is directed by the C-terminal one-third of Molo- ney murine leukemia ... See full document
8
Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase.
... RNases H to degrade their genomic RNA present in RNA-DNA hybrid ...the 3 9 end of the minus-strand strong- stop DNA and the 3 9 end of the genomic RNA is ...with RNase A in either low (TE) or ... See full document
13
Expression of Moloney Murine Leukemia Virus RNase H Rescues the Growth Defect of an Escherichia coliMutant
... RNases H degrade the RNA strand of RNA-DNA hetero- duplexes, and functional isoforms of the enzymes such as re- verse transcriptase (RT) have been identified in many systems (2, 4, 6, ...RT activity ... See full document
6
RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity.
... active RNase H protein band was detected as a cleared area on the resulting autoradiogram or as a dark band in the negative image shown in ...with RNase H activity ...coli RNase ... See full document
9
Mutations in HIV-1 Reverse Transcriptase Affect the Errors Made in a Single Cycle of Viral Replication
... a single virus, enough mutations occur within a few years of infection to generate a swarm of related viruses with differing genomic se- ...during viral replication. Understand- ing the ... See full document
13
Reverse transcriptase activity per virion for avian myeloblastosis virus and Rauscher murine leukemia virus.
... The remaining ma42 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have measured reverse transcriptase enzyme activity per virus particle for samples of avian myelob[r] ... See full document
6
Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase.
... 1 Isolation and Properties of Moloney Murine Leukemia Virus Mutants: Use of a Rapid Assay for Release of Virion Reverse Transcriptase Received 25 November 1980/Accepted 7 January 1981 A [r] ... See full document
10
Selection of Mutations in the Connection and RNase H Domains of Human Immunodeficiency Virus Type 1 Reverse Transcriptase That Increase Resistance to 3′-Azido-3′-Dideoxythymidine
... and RNase H domains of ...the mutations D549N and H539N, which decrease RT RNase H activity, also increase resistance to AZT ...H539N mutations increased AZT resistance by ... See full document
8
Selection of Optimal Polypurine Tract Region Sequences during Moloney Murine Leukemia Virus Replication
... (PPT) region of viral RNA. In this study, we tested replication properties for Moloney murine leukemia viruses with targeted mutations in the PPT and in conserved ... See full document
11
Characterization of Intracellular Reverse Transcription Complexes of Moloney Murine Leukemia Virus
... of reverse transcrip- tion of the RNA genome into DNA is carried out in the cytoplasm soon after viral penetration into the cell and is generally completed within 8 to 12 h ...which reverse ... See full document
7
Analysis of mutant Moloney murine leukemia viruses containing linker insertion mutations in the 3' region of pol.
... To confirm this observation for the linker insertion mutants, NIH 3T3 cells were infected with representative mutant viruses and assayed for the ability to synthesize all three forms of [r] ... See full document
7
Contributions of DNA polymerase subdomains to the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase.
... 5721 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Previous studies showed that an isolated human immunodeficiency virus type 1 HIV-1 RNase H domain expressed as a fus[r] ... See full document
9
Structure-Based Moloney Murine Leukemia Virus Reverse Transcriptase Mutants with Altered Intracellular Direct-Repeat Deletion Frequencies
... Mutants were also tested using a 284-base duplication in a different region of lacZ (Fig. 4B). The average wild-type dele- tion rate for this repeat was 27.2%. Deletion frequencies for most mutants differed less ... See full document
8
Related subjects