[PDF] Top 20 Isolation and preliminary characterization of temperature-sensitive mutants of encephalomyocarditis virus.

... I have isolated temperature-sensitive mutants of encephalomyocarditis EMC virus because I wished to find mutants where the cleavage of viral precursor proteins is defective in mutant-inf[r] ... See full document

... Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation.. Influenza viral RNA and ribonucleopro[r] ... See full document

... 27/35 of all the mutants tested thus far [unpubdata ] are RNA+ is similar to that found tants; group C contains one RNA+ mutant that lished ts mutants of vesicular stomatitis virus, a is[r] ... See full document

... Mutants were assigned to complementation groups by observing lysis at 29 C in bacteria mixedly infected with two temperature-sensitive mutants.. The mixture was left for 30 min for adsor[r] ... See full document

... When the RNA species produced during infection by either wild-type virus or tsB9 at the permissive temperature were examined by agarose gel electrophoresis, no difference was seen.. At t[r] ... See full document

... Little or no infectious virus was produced in cells infected with group I or group III mutants and shifted to 33.5 C in the presence of 5-AC, indicating little or no virion RNA synthesis[r] ... See full document

... Copyright 1971 American Society for Microbiology Isolation and Characterization of TemperatureSensitive Mutants of Vesicular Stomatitis Virus, New Jersey Serotype Received for publicati[r] ... See full document

... The means calculated elevated temperature, and only 16% of the polyfrom several experiments for the cellular DNA merizing activity remained after 20 min at 45°C, synthesized at 39.5°C re[r] ... See full document

... The group IVb mutants Table 2 do not form plaques at 32.5°C yet produce cytopathic effects which include the occasional appearance of cells containing polyhedral occlusion bodies at 32.5[r] ... See full document

... Synthesis of the viral DNA strand was shown to be diminished for both wild-type virus and these mutants at the restrictive temperature of 39.5 C, but the mutants were more defective than[r] ... See full document

... Other experiments data not shown have indicated that cells infected with ts-8 or ts-9 at 39 C reproducibly yield significant amounts of virus-specific RNA, but the amount of RNA synthesi[r] ... See full document

... Preliminary Physiological Characterization of Temperature-Sensitive Mutants of Vesicular Stomatitis Virus Different temperature-sensitive mutants of vesicular stomatitis virus have been [r] ... See full document

... Reproducibility of recombination frequencies Recombination frequencies % Cross Expt 2 Expt 3 Mean 34 8.3 28 28 0.12 NDa 36 7 38 24 0.12 21 34 8.0 35 23 0.11 26 shown to occasionally prod[r] ... See full document

... From these data it is apparent that mutants belonging to complementation groups D, E, and F are defective in functions required for virus-specific RNA synthesis throughout the replicativ[r] ... See full document

... HeLa cells were infected with ts206 at the permissive and nonpermissive temperatures, and the cells were maintained at either temperature and pulse labeled with [3H]uridine as described [r] ... See full document

... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document

... Among these mutants, we distinguished at least three phenotypes: i mutants that were defective in their ability to rescue or replicate the AAV genome from the recombinant plasmids rep mu[r] ... See full document

... SDS-PAGE analysis of extracts of Vero or n-33 cells infected at a multiplicity of infection of 20 PFU per cell with hr259, hr79, or HSV-2 186 and labeled with [35S]methionine from 3 to 1[r] ... See full document

... It can be seen that ts 3 fails to synthesize any of the capsid antigens; ts 11, ts 4, and ts 7 representing three distinct complementation groups produced normal precipitin lines corresp[r] ... See full document

... After ts mutants had been selected in BK tissue and stocks of virus prepared in these cells, further characterization was performed in a heteroploid cell line HeLa in which RS virus prod[r] ... See full document