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[PDF] Top 20 Multicenter Comparison of Different Real Time PCR Assays for Quantitative Detection of Epstein Barr Virus

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Multicenter Comparison of Different Real Time PCR Assays for Quantitative Detection of Epstein Barr Virus

Multicenter Comparison of Different Real Time PCR Assays for Quantitative Detection of Epstein Barr Virus

... a different quantitative EBV PCR ...performed quantitative EBV testing on all panels using their own protocols, reagents, controls, and ...the quantitative calibrators used, and the ... See full document

7

Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens

Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens

... load assays, we prefer to use un- fractionated whole-blood since it combines all components that may harbor EBV, ...using real-time PCR have reported that whole-blood DNA preparations may ... See full document

7

Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus

Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus

... a comparison using only one assay design targeting only one of a number of viruses for which such a method may have clinical ...comparator assays and other viruses, particularly those with RNA ...of ... See full document

7

Interlaboratory Comparison of Six Real Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA

Interlaboratory Comparison of Six Real Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA

... Diagnostic sensitivity (DxSn) was estimated for each particular qPCR assay. Of all 57 positive samples that constituted the panel, 56 (98%), 53 (93%), 49 (86%), 47 (82%), 45 (79%), and 32 (56%) samples were scored as ... See full document

11

Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients

Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients

... 23 real-time PCR assays based on TaqMan technology has been developed for the detection and monitoring of 16 different viruses and virus families including human ... See full document

10

Comparison of Commercial Extraction Systems and PCR Assays for Quantification of Epstein Barr Virus DNA Load in Whole Blood

Comparison of Commercial Extraction Systems and PCR Assays for Quantification of Epstein Barr Virus DNA Load in Whole Blood

... of real-time quantitative PCR, measurement of EBV DNA loads during these EBV-associ- ated diseases has been largely implemented in clinical practice (6, ...the different PCR ... See full document

6

Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children

Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children

... using quantitative PCR methods and including clinical data are being performed to determine what AdV viral load is considered significant for a pathogenic role in respiratory ...illness. Detection of ... See full document

7

Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples

Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples

... State University Veterinary Diagnostic Laboratory (ISU-VDL). These samples originated on 86 farms located in 12 U.S. states: Iowa, Illinois, Indiana, Minnesota, Missouri, North Carolina, North Dakota, Nebraska, Ohio, ... See full document

7

Real time PCR assays for hepatitis B virus DNA quantification may require two different targets

Real time PCR assays for hepatitis B virus DNA quantification may require two different targets

... same time, it also dem- onstrated the necessity of using two pairs of primers and two probes for the quantitative detection of HBV DNA, which is highly ...duplex real-time PCR ... See full document

9

Development of a Quantitative Real Time Detection Assay for Hepatitis B Virus DNA and Comparison with Two Commercial Assays

Development of a Quantitative Real Time Detection Assay for Hepatitis B Virus DNA and Comparison with Two Commercial Assays

... in-house assays, sample preparation is still the most difficult step to perform and to ...for real-time PCR detection assays leave the input DNA as the only unknown ... See full document

5

Comparison of Loop Mediated Isothermal Amplification and Real Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices

Comparison of Loop Mediated Isothermal Amplification and Real Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices

... the time of specimen collection as possible and by DNA elution in ...of detection of the LAMP assay using ...LAMP assays for Mycobacterium tuberculosis and influenza virus, this would simplify ... See full document

9

Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis

Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis

... immunosorbent assays (ELISAs) [6]. However, these assays may be limited by false-negative results in early or chronic cases of infection ...between different laboratories ...[10–13]. ... See full document

10

Comparison of Real Time PCR and Antigen Assays for Detection of Hepatitis E Virus in Blood Donors

Comparison of Real Time PCR and Antigen Assays for Detection of Hepatitis E Virus in Blood Donors

... early detection of viral infection prior to the occurrence of IgM antibodies, would enhance the management of HEV ...the detection of anti-HEV IgM (Wantai HEV-Ag assay ...the detection of HEV-Ag ... See full document

7

Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays

Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays

... interassay comparison, each of the five assays detected purified ...from real patients as much as possible, mock- infected sputum was prepared by spiking it with whole ...in different ... See full document

9

Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay

Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay

... a PCR Thermal Cycler ...qualitative PCR assay could detect two copies of a plasmid control that contained the BamHI W ...qualitative PCR was more sensitive than the real-time PCR ... See full document

5

Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA

Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA

... The results of intra- and interassay variability support the belief that, for patient monitoring, confidence intervals are required to report results instead of plain absolute values for viral load. Otherwise, ... See full document

5

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

... a real time PCR panel for detection of herpesvirus DNA in CSF with methods employing nested ...two assays were observed for HSV-1 and EBV analysis, where one negative sample was found ... See full document

5

Progress in Quantitative Viral Load Testing: Variability and Impact of the WHO Quantitative International Standards

Progress in Quantitative Viral Load Testing: Variability and Impact of the WHO Quantitative International Standards

... international quantitative standards can be of great benefit in improving consensus among testing laboratories (1, 17, ...load assays without a currently available international standard speaks to the ... See full document

8

Detection of Epstein-Barr virus (EBV) in human lymphoma tissue by a novel microbial detection array

Detection of Epstein-Barr virus (EBV) in human lymphoma tissue by a novel microbial detection array

... the Epstein-Barr virus (EBV), are associated with 10-15% of B-cell lymphomas and are found at a higher frequency in immunosuppressed ...Microbial Detection Array (LLMDA), a comprehensive ... See full document

7

Diagnosis of Medically Important Viruses: A Molecular Approach

Diagnosis of Medically Important Viruses: A Molecular Approach

... and quantitative molecular virus assays, based on PCR technology could identify active infection by detecting viral messenger RNA (mRNA) but the most widely used diagnostic tests are ... See full document

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