[PDF] Top 20 Multilocus Sequence Typing for Differentiation of Strains of Candida tropicalis

... of strains might appear as A, T, or W ( ⫽ A ⫹ ...C. tropicalis alleles were therefore conjoined into a single sequence, and then each base in the sequence was rewritten twice for a homozygous ... See full document

... 61 Candida tropicalis isolates from 43 patients (28 adults and 15 children) diagnosed with candidemia at two teaching hospitals in São Paulo, Brazil, were characterized by multilocus sequence ... See full document

... Recently, multilocus sequence typing (MLST) has been de- veloped to meet the increasing need for global surveillance and comparison of genotypes in a central database via the ...diploid ... See full document

... the differentiation of strains involved in food-borne outbreaks of salmonellosis, including strains within the same serotype that are strongly clonal and that may therefore not be differentiable by ... See full document

... Although MLST has been developed as a tool for the typing of C. albicans, it had never been formally compared to the techniques already established for the study of C. albicans. Our results presented here show ... See full document

... of Candida albicans isolates is essential for understanding the epidemiology of nosocomial infections caused by this ...of multilocus sequence typing (MLST) for characterizing ... See full document

... MLP typing, the 50 isolates studied yielded 38 different profiles, with a discriminatory power index of ...MLST typing, the 50 isolates yielded 40 distinct DSTs, with a discriminatory power index of ...to ... See full document

... of strains might appear as A, T, or W (⫽ A ⫹ ...single sequence, and then each base in the sequence was rewritten twice for a homozygous (A, C, G, or T) datum or once each for the two component bases ... See full document

... 42), multilocus enzyme electrophoresis (MLEE) (5), and fingerprinting with complex DNA probes ...direct sequence comparison for typing ...direct sequence comparison, we developed a ... See full document

... africana strains exhibit a phenotypic and genetic homogeneity that is dif- ferent from their country of origin ...of sequence change also suggests that this typing technique may not be ideal for ... See full document

... (“mutator”) strains is well recognized among some species of bacteria, particularly gram-negative bacilli (18, 59, ...variable strains exist among pathogenic Candida spe- ...some strains at ... See full document

... suis strains, a multilocus sequence typing (MLST) method was ...strain typing of H. suis. In addition to the 10 unique sequence types (STs) among the 10 isolates grown in vitro, ... See full document

... C. tropicalis), seven (for ...C. tropicalis). Each sequence is compared with the reference database for the ...existing sequence, it is assigned that sequence’s allele number ...C. ... See full document

... strain typing have been developed for this species (32), but no system has yet achieved universal ...sequences. Typing based on the intergenic transcribed spacer sequences of genes encoding rRNA tend to ... See full document

... between strains originating from human and ...human strains from both clusters were identical to bovine isolates is a strong indication of a possible link between the two ...mal strains within the ... See full document

... for typing and can even replace PFGE ...the sequence type (ST) of each isolate is defined by the alleles present at each distinct ...that sequence data are portable and can be readily com- pared ... See full document

... A total of 68 C. jejuni and 20 C. coli clinical isolates were collected from infected children up to 14 years from 5 general hospitals of the area of Attica. All the strains were isolated from sporadic cases with ... See full document

... 308 strains included were mostly isolated from skin biopsies, in agreement with previous data showing that ...these strains were involved in ...supplementary strains corresponding to these STs, apart ... See full document

... a typing scheme such as MLST might be very valuable for the study of the eventual association of virulence with specific ...the strains from Germany (22), only two alleles were found in our global strain ... See full document

... Test product solutions of certain concentrations (determined by their respective MICs) were used. Initially, 100 μL of SDB was added to the wells. Then, 50 μL of each test product at various concentrations (MIC x 8, MIC ... See full document