[PDF] Top 20 New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... a new detection platform ...DNA polymerase based reaction components, with a HDA amplification cocktail, which is now commercially available in various formats from Biohelix Corp, ... See full document
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... per reaction (500 copies/ml ...This real time PCR assay allows rapid, sensitive and specific quantification of all currently defined adenoviruses into either two or one multiplex assay for clinical ... See full document
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Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion
... digital polymerase chain reaction (ddPCR) has been shown to be advantageous compared to real-time quantitative PCR due to its in- creased accuracy, reproducibility, and ... See full document
14
Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... The FQ-PCR amplification curves and the corresponding FQ-PCR standard curve (Figure 1) were generated by employing the successively diluted known copy numbers of pVP3 for real-time PCR reaction under ... See full document
7
miR-486-5p suppresses prostate cancer metastasis by targeting Snail and regulating epithelial–mesenchymal transition
... C4-2. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results demon- strated that the metastatic C-4 cell line showed significantly decreased ... See full document
6
Long noncoding RNA CASC2 predicts the prognosis of glioma patients and functions as a suppressor for gliomas by suppressing Wnt/β-catenin signaling pathway
... Notes: (A–C) β-catenin, cyclin-D1, and c-Myc were upregulated in glioma tissues compared with adjacent normal brain tissues, as found by qrT-Pcr analysis. (D) Overexpression of casc2 suppressed the protein expression of ... See full document
9
<p>Effects of dynein light chain Tctex-type 3 on the biological behavior of ovarian cancer</p>
... by quantitative real-time polymerase chain reaction and Western blotting respectively, and the proliferation, apoptosis, migration and invasion of SKOV3 cells after DYNLT3 ... See full document
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The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer
... Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of ... See full document
12
Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real time polymerase chain reaction
... Each reaction contained 5 µL DNA template from disc extracts, 10 µL of LightCycler 480 SYBR Green I Mas- ter (Roche diagnostics Gmbh, Germany), 1 µL of 10 µM forward and reverse primer ...the reaction mix ... See full document
5
Original Article NF-κB and STAT6 signaling transduction pathways in immunoglobulin E production of U266 cell
... Using quantitative real-time polymerase chain reaction, Western blotting, and enzyme-linked immunosorbent assays, we found that the U266 cell stimulated with the cytokine ... See full document
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Quantitative Real-time Polymerase Chain Reaction for Tracking Microbial Gene Expression in Complex Environmental Matrices
... Viability status of the target microorganism is extremely critical to accurately determine the biosafety status of a food sample (Keer and Birch, 2003). The conventional cultivation protocol even though highly ... See full document
14
Analyzing the gene expression profile of pediatric acute myeloid leukemia with real-time PCR arrays
... by quantitative real-time polymerase chain reaction in 94 consecutive childhood acute lymphoblastic leukaemia (ALL) samples and its association with clinical/biological features ... See full document
12
Clinicopathological and prognostic significance of long noncoding RNA MALAT1 in human cancers: a review and meta-analysis
... qRT-PCR: quantitative real-time polymerase chain reaction; ISH: in situ hybridization; HWE: hardy–weinberg equilibrium; HR: hazard ratio; OS: overall survival; DFS: disease-free ... See full document
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... Chlamydia trachomatis (C. trachomatis) is an intracellular bacterium implicated in reproduc- tive tract infections. It is also the pathogen re- sponsible for the most common sexually trans- mitted disease, worldwide, in ... See full document
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Seven-microRNA panel for lung adenocarcinoma early diagnosis in patients presenting with ground-glass nodules
... Methods: To identify potential miRNAs of interest, we analyzed the miRNA expression profile of tumor and adjacent non-para-tumor tissue in three participants by next-generation sequencing (NGS). We then assessed the ... See full document
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... results from the four algorithms an integration tool for analyzing reference genes expression was used http:// www.leonxie.com/referencegene.php. First according to the reference genes ranking by every algorithm from the ... See full document
9
LincRNA-ROR promotes metastasis and invasion of esophageal squamous cell carcinoma by regulating miR-145/FSCN1
... Patients and methods: ROR expression in 91 pairs of ESCC tissue samples and matched adjacent tissues was quantified with real-time fluorescent quantitative polymerase chain ... See full document
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Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ... See full document
16
Regeneration and reprogramming compared
... by quantitative real time polymerase chain reaction (qPCR) of regenerating and non-regenerating tissue compared to an embryonic, pluripotent reference cell type, analysis of the ... See full document
14
Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... and real-time PCR, and detection based on DNA or ...products. Real time PCR is a more recently developed technique compared to endpoint ...This new generation of PCR adds an optical ... See full document
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