[PDF] Top 20 Nucleotide substitutions within U5 are critical for efficient reverse transcription of human immunodeficiency virus type 1 with a primer binding site complementary to tRNA(His).
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Nucleotide substitutions within U5 are critical for efficient reverse transcription of human immunodeficiency virus type 1 with a primer binding site complementary to tRNA(His).
... of virus from cultures transfected with pHXB2(His- AC-GAC) and pHXB2(His-AC-TGT) with the wild-type ...of virus were similar, with the virus derived from pHXB2(His-AC-TGT) slightly delayed ... See full document
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Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).
... eral nucleotide changes were also common to all of the PBS clones analyzed from the viruses derived from pHXB2(His- ...first nucleotide 5 9 of the PBS (nucleotide ...wild type, we favor the ... See full document
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Human immunodeficiency virus type 1 can use different tRNAs as primers for reverse transcription but selectively maintains a primer binding site complementary to tRNA(3Lys).
... from reverse transcription, mutant proviral genomes were constructed in which the PBS region was altered so that proviruses which were generated as a result of reverse transcription would pos- ... See full document
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Genetic Analysis of a Unique Human Immunodeficiency Virus Type 1 (HIV-1) with a Primer Binding Site Complementary to tRNAMet Supports a Role for U5-PBS Stem-Loop RNA Structures in Initiation of HIV-1 Reverse Transcription
... a virus which reverts to restore the CCCT motif ...disrupted tRNA structure necessary to form an initiation complex similar to what has been found by Isel et ...interactions within the viral genome ... See full document
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The Human Immunodeficiency Virus Type 1 Gag Polyprotein Has Nucleic Acid Chaperone Activity: Possible Role in Dimerization of Genomic RNA and Placement of tRNA on the Primer Binding Site
... cellular tRNA molecule is annealed to an 18-base complementary region (the primer binding site, or PBS) on the genomic RNA, where it will serve as primer for reverse ...a ... See full document
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A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription.
... wild type and TAR revertants to undergo reverse ...During reverse transcription, DNA synthesis is initiated at the PBS site, con- tinues through the U5 region of the LTR, and ... See full document
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Identification of sequences downstream of the primer binding site that are important for efficient replication of human immunodeficiency virus type 1.
... Reverse transcription of retroviruses is initiated from an 18-nucleotide (nt) primer binding site (PBS), located within the 5 * region of viral genomic RNA, to which the ... See full document
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Identification of a sequence within U5 required for human immunodeficiency virus type 1 to stably maintain a primer binding site complementary to tRNA(Met).
... tiate reverse transcription ...a tRNA might determine the use of the tRNA to initiate reverse ...initiate reverse transcription (4, 16, 34, ...wild-type ... See full document
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Mutations in the U5 Region Adjacent to the Primer Binding Site Affect tRNA Cleavage by Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo
... the tRNA between the terminal rA and the adjacent rC, 1 nucleotide from the RNA-DNA junction ...single nucleotide (G) that lies between the CA dinucleotide and the PBS was deleted ...occurred ... See full document
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Deletions in the tRNA(Lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcription.
... The interaction between the PBS region and the cellular tRNA is a critical step in the reverse transcription of One of the early steps in the replication of human immunodeficiency virus [r] ... See full document
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Reduced replication of human immunodeficiency virus type 1 mutants that use reverse transcription primers other than the natural tRNA(3Lys).
... Characterization of transiently produced virions. All PBS- replacement viruses showed reduced replication rates and could not be stably maintained because of reversion. In order to study the mutant viruses in more ... See full document
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The importance of the A-rich loop in human immunodeficiency virus type 1 reverse transcription and infectivity.
... for 30 min at 37°C to eliminate potentially contaminating plasmid DNA. At the same time, cells were exposed to heat-inactivated viruses as a negative control. After 2 h, the cells were washed twice with serum-free RPMI ... See full document
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Cell Factors Stimulate Human Immunodeficiency Virus Type 1 Reverse Transcription In Vitro
... and reverse tran- scription products were detected in the ...strong-stop reverse transcription products was detected (peak R in ...strand transcription products was greatly increased by the ... See full document
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The Role of Nucleocapsid and U5 Stem/A-Rich Loop Sequences in tRNA3Lys Genomic Placement and Initiation of Reverse Transcription in Human Immunodeficiency Virus Type 1
... vitro reverse transcription studies, which in- dicate that RT shows lower processivity during the initiation phase of reverse transcription than during the elongation phase ...the U5 ... See full document
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Kinetics of human immunodeficiency virus type 1 reverse transcription in blood mononuclear phagocytes are slowed by limitations of nucleotide precursors.
... Rates of full-length double-stranded retroviral DNA formation by reverse transcription intracellular reaction Virus HIV-1W13 HIV-lJR-csF ASVa HIV-lJR-FL HIV-lJR-FL Cell type Rate nt/min [r] ... See full document
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Characterization of Intracellular Reverse Transcription Complexes of Human Immunodeficiency Virus Type 1
... elongated reverse transcription products, ali- quots from the same fractions were subjected to PCR using a forward primer located on the 5⬘ long terminal repeat U5 region and a reverse ... See full document
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The Tat Protein of Human Immunodeficiency Virus Type 1 (HIV-1) Can Promote Placement of tRNA Primer onto Viral RNA and Suppress Later DNA Polymerization in HIV-1 Reverse Transcription
... a 1-exon form of Tat markedly suppressed RT ...wild- type Tat 86, but not the mutant proteins missing either the cysteine-rich, core, or basic domain or the glutamine-rich re- gion, displayed RNA-annealing ... See full document
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Inhibition of Human Immunodeficiency Virus Type 1 Replication prior to Reverse Transcription by Influenza Virus Stimulation
... A virus-stimulated supernatants, we performed blocking experiments using neutralizing antibodies against these cyto- ...A virus-stimulated supernatants did not reduce their HIV-inhibitory effect, which ... See full document
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Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Target Distinct Phases of Early Reverse Transcription
... pocket within the enzyme (31), causing a conformational change that disrupts the catalytic site and blocks its DNA polymerase ...of virus isolated from patients taking NVP ...commercial ... See full document
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Cross-Linking of the Fingers Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase to Template-Primer
... The cross-linking data show that the extended template can come quite close to the fingers of p66, but do not define the interactions between the fingers and template. It is possible that there are no specific ... See full document
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