[PDF] Top 20 Optimization of Routine Identification of Clinically Relevant Gram Negative Bacteria by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and the Bruker Biotyper
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Optimization of Routine Identification of Clinically Relevant Gram Negative Bacteria by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and the Bruker Biotyper
... ual identification methods. In parallel to routine processing, colonies were applied to a MALDI-TOF target as part of the normal workflow and were batch processed for MS analysis at the end of the ...The ... See full document
9
Identification of Anaerobic Bacteria by Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with On Plate Formic Acid Preparation
... anaerobic bacteria were obtained from the Mayo Clinic Bacteriology Laboratory over a 6-month ...other identification performed by sequencing of the first 500 bases of the 16S rRNA gene as described ... See full document
5
Dermatophyte Identification Using Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... the Bruker Biotyper matrix-assisted laser desorption ionization–time of flight (MALDI- TOF) mass spectrometer (MS) for the identification of ... See full document
5
Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... “common” bacteria, we found the manufac- turer’s recommendations overly stringent, such that identifica- tion scores between ...identify bacteria to the species level, particularly when sup- ported by ... See full document
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Evaluation of a Semiquantitative Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Rapid Antimicrobial Susceptibility Testing of Positive Blood Cultures
... incubation time, concentration of the substance, and the antibiotic-specific time-kill ...with bacteria extracted from the anaerobic ...with bacteria that were extracted from the aerobic and ... See full document
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Comparison of Direct Colony Method versus Extraction Method for Identification of Gram Positive Cocci by Use of Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... for Gram-positive and Gram-negative ...the Gram-positive cell wall using the direct colony method may not always be suffi- cient to adequately prepare proteins for detection by MALDI- TOF MS ... See full document
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Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... nitrogen laser. The spectra were re- corded in the linear mode in a mass range of 2 to 20 kDa and subsequently analyzed using MALDI Biotyper Automation Control and Biotyper ...the time, ... See full document
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Comparison of the Vitek MS and Bruker Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Rhodococcus equi and Dietzia spp
... environmental bacteria that have rarely been associated with human ...their Gram stain morphology and colony appearance is problematic. Matrix-assisted laser desorption ... See full document
6
Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) also known as Bruker MALDI Biotyper is a technology ... See full document
105
Evaluation of the Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi
... traditional identification methods, especially with sterile molds and species that are morphologically similar but may differ ...using routine phenotypic identification in the clinical labo- ...is ... See full document
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Rapid Identification of Clinically Relevant Members of the Genus Exophiala by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Description of Two Novel Species, Exophiala campbellii and Exophiala lavatrina
... reference mass spectra (MSPs), exactly as described in Materials and ...existing Bruker database for the identification of the remaining isolates, which were processed in an operator-blinded manner using the ... See full document
15
Evaluation of a Simple Protein Extraction Method for Species Identification of Clinically Relevant Staphylococci by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... atrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been used to identify various microorganisms in clinical ... See full document
5
Interlaboratory Comparison of Sample Preparation Methods, Database Expansions, and Cutoff Values for Identification of Yeasts by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a Yeast Test Panel
... using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to determine the identification of clinically ... See full document
7
Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... phenotypic identification of ⬎40 currently recognized CoNS species re- mained a challenge, because the technique was labor-intensive and uncommon CoNS were usually not identified (5, ...CoNS identification ... See full document
7
Optimizing Identification of Clinically Relevant Gram Positive Organisms by Use of the Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System
... evaluating Gram-positive and Gram-negative organisms, as well as yeast (19, 27, ...bacterial identification compared to direct spotting of bacteria without a formic acid ...workflow. ... See full document
7
Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... MALDI matrix (a saturated solution of ␣ -cyano-4-hydroxy-cinnamic acid in 50% ...temperature. Mass spectra were acquired using the MALDI-TOF spectrometer in a linear positive mode (Mi- croflex; ... See full document
7
Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... stock Bruker Biotyper data- ...the Biotyper database, namely, Gordonia amarae, ...The Bruker Biotyper database used in this study (DB 4613) contains 70 different Corynebacterium ... See full document
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Evaluation of the Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Blood Isolates of Acinetobacter Species
... available matrix-as- sisted laser desorption ionization–time of flight mass spectrome- try (MALDI-TOF MS) systems are rapidly emerging novel technology widely used in ... See full document
6
Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for ... See full document
9
Evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by MALDI TOF
... centage identification to species level in monomicrobial cultures is variable across the studies from 56 to 100% and probably reflects the different methodologies and gold standards with which it was compared as ... See full document
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