[PDF] Top 20 Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
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Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... CSFV detection, its use as a screening test, with complete blood samples, in clinically healthy pig herds showed little predictive value, with ...CSFV-specific real-time RT-PCR. (B) ... See full document
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Detection of nucleic acid of classical swine fever virus by reverse transcription loop mediated isothermal amplification (RT LAMP)
... for rapid detection and typing of emerging viruses such as Severe acute respiratory syndrome coronavirus [17], Japanese encephalitis virus [18], Pseudorabies virus [19] and Classical ... See full document
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Design and Performance of the CDC Real Time Reverse Transcriptase PCR Swine Flu Panel for Detection of 2009 A (H1N1) Pandemic Influenza Virus
... first swine influenza virus (SIV), also referred to as classical SIV H1N1 virus, was isolated in the United States in 1930 and circulated in pigs for at least 60 years (22, ...and swine ... See full document
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Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... hemorrhagic fever (DHF) and DEN shock syndrome (DSS), which are more serious manifestations often associated with secondary DEN virus infections ...DEN virus serotypes may be circulating simulta- ... See full document
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Evaluation of a Rapid and Completely Automated Real Time Reverse Transcriptase PCR Assay for Diagnosis of Enteroviral Meningitis
... EV assay on CSF specimens obtained by lumbar puncture from patients with signs and symptoms of meningitis as part of their routine evalua- ...EV assay at the trial sites, and by two laboratory-developed ... See full document
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Preclinical Diagnosis of African Swine Fever in Contact Exposed Swine by a Real Time PCR Assay
... other swine diseases (20). Rapid and precise detection of ASFV is critical for disease ...including detection of infectious virus, viral antigens, and spe- cific antibodies and ... See full document
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Development of a Real Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
... influenza virus isolates representing all HA subtypes (see Table 6) including both avian North American and Eurasian lineage viruses and isolates of human, equine, and swine origin to demonstrate ... See full document
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Real Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages
... The virus has been circulating in humans for at least 50 ...syncytial virus (RSV), which is the most common cause of respiratory tract disease in children (3, 4, 15, ...and rapid diagnostic test for ... See full document
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Two Novel Real Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination in Platelet Concentrates
... Two real-time reverse transcriptase PCR (RT-PCR) assays performed in a LightCycler instrument were developed and compared regarding specificity and sensitivity by the use of ... See full document
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Development of an Internally Controlled Real Time Reverse Transcriptase PCR Assay for Pan Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays
... engue virus (DENV) is the most common vector-borne hu- man viral pathogen worldwide ...related virus serotypes (designated DENV-1 to -4) results in a range of clinical manifestations span- ning asymptomatic ... See full document
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Real Time Reverse Transcription PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens
... mumps virus is a negative-strand RNA virus in the family ...including fever, headache, and myalgia, followed by swelling of the salivary ...the detection of immunoglobulin M-specific ... See full document
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Rapid and Sensitive Method Using Multiplex Real Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4
... by virus isolation in cell culture and immunofluorescent assays. Reverse transcriptase PCR is now recognized as a sensitive and specific alternative for detection of respiratory RNA ... See full document
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Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... available, rapid and accurate diagnosis of current and future EV-D68 infections is of great concern to clinicians and public health ...RT- PCR assay we have developed can be used for epidemiological ... See full document
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Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
... Although real-time RT-PCR assays targeting conserved re- gions can provide accurate detection of the HEV genomes and yield results more rapidly than conventional RT-PCR, commonly, a ... See full document
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... This real-time PCR, which could be completed in 1 h, was a rapid method allowing the fast detection and confirmation of dengue viruses in clinical ...This rapid, early, and ... See full document
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Rapid Genome Detection of Schmallenberg Virus and Bovine Viral Diarrhea Virus by Use of Isothermal Amplification Methods and High Speed Real Time Reverse Transcriptase PCR
... Recombinase polymerase amplification. Sequences of primers and probes used for RPA, as well as details of the assay design are shown in Tables 1 and 2, respectively. Both TwistAmp exo RPA probes were syn- thesized ... See full document
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Detection of Anaplasma marginale and A phagocytophilum in Bovine Peripheral Blood Samples by Duplex Real Time Reverse Transcriptase PCR Assay
... qRT-PCR assay performance and the quality of the RNA extraction ...qPCR assay results when reverse transcriptase (SuperScript III reverse transcriptase; Invitrogen ... See full document
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Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... The PCR primers and MB were designed based on the sequence of the CCR5 gene (GenBank accession ...the real-time RT-PCR, the mRNA of a housekeeping gene, GAPDH, was ... See full document
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... by Reverse Transcription reaction and amplification by PCR assay for determining the sensitivity of the optimum pool size in the detection of infective ...The Reverse transcription ... See full document
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Comparison of Methods for Detection of Vaccinia Virus in Patient Specimens
... vaccinia virus and the Harvey strain of variola virus could be inactivated after 45 min of acetone treatment ...vaccinia virus to follow the most current recommendations from the Centers for Disease ... See full document
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