[PDF] Top 20 Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.

... Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.. Simian virus 40 deoxyribonucleic acid synthe[r] ... See full document

... Several T-antigen activities, such as origin binding, viral DNA replication, ATPase activity, stimulation of cell DNA synthesis, stimulation of rDNA transcription, transformation, and nu[r] ... See full document

... Simian virus 40 (SV40) large tumor (T) antigen is the major regulatory protein that directs the course of viral infection, primarily by interacting with host cell proteins and ... See full document

... patch mutants for their abilities to replicate in BSC40 cells, which are permissive for viral ...patch mutants were defective for viral replication (data not ...this replication de- fect is ... See full document

... in transformation, as LT mutants defective in binding to the SV40 origin of replication transform cells with the same efficiency as wild-type LT (10, 55, 56, ...cellular transformation cannot ... See full document

... multifunctional large T antigen, which contains 708 amino acids, plays vital roles in coordinating viral DNA repli- cation and transformation (6, 18, 47, ...of T antigen include ... See full document

... DNA replication by 5- to 10-fold, whereas mutations at positions 3, 4, and 5 completely abolished it ...in vitro data showing that changing position 1 from a G to an A reduces binding by approximately ... See full document

... Such a change in conformation could affect multiple independent functions, including origin binding, transformation, and the function of the active site element of the zinc finger in rep[r] ... See full document

... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document

... To determine whether the inability of these deletion mutants to transform cells was due to the short half-life of the protein within the cell and not to the disruption of a potential tra[r] ... See full document

... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document

... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document

... However, mutants which lack origin-binding activity retain the ability to transform both primary and established cells phenotypically, indicating that It has been known for some time tha[r] ... See full document

... By cotransfecting CV-1 cells with an origin-defective plasmid encoding wild-type T antigen and a replicationcompetent plasmid encoding mutant T antigen, we saw that the level of interfer[r] ... See full document

... We report here the isolation and characterization of in vitro-constructed amino acid substitu- The large tumor antigen T antigen of simian virus 40 SV40 is a regulatory protein that is e[r] ... See full document

... 2195-2207 0022-538X/92/042195-13$02.00/0 Copyright © 1992, American Society for Microbiology A Deletion in the Simian Virus 40 Large T Antigen Impairs Lytic Replication in Monkey Cells I[r] ... See full document

... Simian virus 40 SV40 DNA codes for two early proteins, the large and small tumor T antigens 22, 30, 33, which can be immunoprecipitated from extracts of cells productively infected or tr[r] ... See full document

... To determine the functional significance of the SV40 lOOK T antigen in SV40 nucleoprotein complexes containing SV40 I DNA, cells were infected with SV40 tsA58, a temperature-sensitive mu[r] ... See full document

... 293-297 0022-538X/85/100293-05$02.00/0 Copyright © 1985, American Society for Microbiology NOTES Formation of a Cruciform Structure at the Simian Virus 40 Replication Origin Abolishes T-[r] ... See full document

... It also plays central roles in immortalization of primary cells 61, 62 and in the malignant Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Linker insertion mutants aff[r] ... See full document