[PDF] Top 20 Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress
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Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress
... erence genes was evaluated using geNorm statistical ...candidate reference genes and mean pair wise variation of each gene from all the reference genes in a given set of sam- ...geNorm ... See full document
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Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar
... and abiotic stress responses ...internal reference genes to be commonly used for normaliza- tion in qRT-PCR of miRNAs and ...keeping genes, such as actin 7 (ACT 7), eukaryotic ... See full document
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Reference Genes for RT qPCR Analysis of Environmentally and Developmentally Regulated Gene Expression in Alfalfa
... candidate reference genes expressed as the geNorm mean pairwise variation (M) of a gene in comparison to the other genes was first tested with samples from plants acclimated to low temperature ... See full document
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<p>Identification and Validation of Reference Genes Selection in Ovarian Cancer Exposed to Hypoxia</p>
... expression genes were GAPDH/TBP in ovarian cancer cells, whereas under hypoxia, the most stable candidate housekeeping gene were RPL13A/ SDHA according to the geNorm algorithm ...treated under ... See full document
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Validation of reference genes for quantitative RT qPCR studies of gene expression in Atlantic cod (Gadus morhua l ) during temperature stress
... the genes chosen are included in the same biological pathway this may interfere with the ...Co-expressed genes will therefore most probably have similar expression ...the genes are co-regulated in ... See full document
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Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR
... comparative analysis of the target genes, both qPCR and HT-SuperSAGE approaches presented a 56% agreement on ...of validation of gene expression results in transcriptomic ...target ... See full document
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Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli
... functional genes in their biosynthe- sis pathways ...functional genes in their biosynthesis pathways [9, 10]. In these reports, analysis of functional genes by reverse transcription ... See full document
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Validation of reference genes from Eucalyptus spp under different stress conditions
... expression analysis by qPCR is the determination of genes that have constitutive expression, which will serve as references in the analysis of plant material from different ...these ... See full document
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Evaluation of reference genes for RT-qPCR study in abalone Haliotis discus hannai during heavy metal overload stress
... the selection of suitable reference genes should be a prerequisite requirement for launching an expression analysis project regarding heavy metal ...stable reference genes from ... See full document
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Selection of reference genes for qPCR in hairy root cultures of peanut
... first validation of reference genes for RT-qPCR in hairy root ...cultures. Selection of an appro- priate RNA extraction method to yield adequate amounts of RNA for RT-PCR ... See full document
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Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures.
... some genes are commonly used and reported as good reference gene in RT- qPCR plant studies, the most traditional ones include actin (ACT) (Maroufi et ...these genes is relative, since ... See full document
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Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)
... The validation of tran- scriptome results via reverse transcription quantitative PCR (RT-qPCR) requires a normalization step for reduc- ing its uncertainties [9, 10], commonly attained by the use of ... See full document
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Microarray based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress
... Six genes that presented high levels of stability in the microarray were tested in RT-qPCR, as well as two other genes commonly used in RT-qPCR data ...possible reference ... See full document
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The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection
... We have further predicted targets of the 66 modulated miRNAs, and note that some differentially expressed miRNAs target a number of immune genes. Then we chose the top three targets of each miRNA to perform GO ... See full document
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Reference genes for normalization of qPCR assays in sugarcane plants under water deficit
... qPCR reactions were performed on the Applied Biosys- tems StepOnePlus System (Foster City CA, USA). The qPCR reactions were optimized by determining the opti- mal primer concentrations (0.2; 0.4; 0.8 μM) ... See full document
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Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
... of genes (3-gene classifier of Weber and 5-gene classifier of Foukakis), our classifier gives better accuracies on validation ...using qPCR, reproduces cases obtained in a routine ... See full document
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Expressed sequenced tags (ESTs) a functional genomic approach for gene discovery
... for abiotic stress-induced sequences obtained from expression profiling studies in Arabidopsis, barley, maize, and ...comparative analysis identified 589 putative stress-responsive ... See full document
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Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements.
... the reference genes ...candidate reference gene with each all of the other genes tested; the result is described as M value (Vandesompele et ...2002). Genes with high variabilities have ... See full document
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... used reference genes can differ among different tissue, organ types or physiological conditions ...improper selection of reference genes will result in inaccurate calculation results ... See full document
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Reference gene selection for gene expression studies using RT qPCR in virus infected planthoppers
... Although no planthopper genonome sequencing pro- ject has yet been announced, great progress has been made with the sequencing of more than 37,000 Nilapar- vata lugens ESTs originated from various tissues [20] and of ... See full document
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