[PDF] Top 20 Sequence requirements of the Epstein-Barr virus latent origin of DNA replication.

... Although region I is required for the transient replication of plasmids bearing region II in EBV-infected B cells, a plasmid lacking region I but containing region II, was observed to re[r] ... See full document

... Unlike the interaction of bEBNA1 with onP, in which one element FR is bound prior to interaction Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest a bEBNAl proteins 1.4 pm[r] ... See full document

... several DNA-protein complexes in vitro within the downstream ...lytic origin of DNA replication of herpes simplex virus type 1, in which DNA- protein interactions which seem to ... See full document

... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ... See full document

... LT, latent); column 6, doubling time (DT); column 7, peak time (PT); columns 8 to 16, activation relative to time zero at indicated times after anti-IgG cross-linking; columns 17 to 19, activation at 48 h relative ... See full document

... total DNA was prepared from these cells and was analyzed by Southern blot hybridization using the same amount of DNA (4 ␮g) and a BamHI-C fragment for a ...EBV DNA was decreased by 28% during 4 days ... See full document

... Z(S186A), a mutant that fails to disrupt EBV latency, binds effi- ciently to the origin of lytic replication. Z(S186A) is defective at activating expression of Rta (34, 37) because of a defect in binding to ... See full document

... EBNA-1 transactivates the latent BamHI C promoter in EBV-negative cells CAT activity normalized in recipient cellsa: Electroporated plasmid Wilson Human fibroblasts 1 1 1 SV2CAT NDb 0.00[r] ... See full document

... 435-bp DNA containing the DS was amplified from clones with wild-type oriP (wt), and a 315-bp product was detected with mutants carrying the deletion (⌬), as ...of DNA from each clone was digested with ... See full document

... unusual sequence elements of EBNA1: the loop- ing domain, the acidic tail, the proline loop, and the WF ...the DNA replication function, indicating that the looping domain plays an essential role in ... See full document

... * 1786 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The Epstein-Barr virus nuclear antigen 1 EBNA1 protein binds and activates the latent replication origin oriP of [r] ... See full document

... (33), replication origin-binding protein MCM4 (30), lamin A (34), histone protein H2AX (59), and interferon regulatory factor 3 ...EBV replication is ... See full document

... lytic replication, but its mechanism of action is also unknown (47, ...lytic replication, the mechanisms govern- ing the cysteine-dependent regulation through C189 have ap- parently not been conserved ... See full document

... DNA replication during EBV lytic replication, we constructed the p2089BKRF3STOP EBV bacmid (BKRF3STOP) by an allelic ex- change procedure ...24.6-kb DNA fragment containing BKRF3 was cut into ... See full document

... Summary of occurrence and binding data for all naturally occurring EBV half-sites Location Family of repeats 15 times 14 times 7 times 4 times Dyad symmetry site Outside site 4 Inside si[r] ... See full document

... OriP replication timing and ...BrdU-labeled DNA specific for the DS (B) or cellular lamin B2 (C) was quantified by anti-BrdU immunoprecipitation and ...A replication timing assay was performed with ... See full document

... the sequence-specific transcription factor RBP-j ␬ (also called RBPJ, CBF1, and CSL) at promoters and enhancers of genes important for B-cell proliferation (17–21; reviewed in references 16 and ...with DNA ... See full document

... a latent state and a lytic replicative cycle in the nuclei of EBV-infected cells ...the latent phase of the EBV life cycle, the EBV genome is maintained as a circular plasmid molecule, which is amplified ... See full document

... coli onC, the SV40 origin, and yeast origins, since the dyad symmetry region contains an easily unwound sequence as well as binding sites for an essential replication protein EBNA1.. How[r] ... See full document

... It seems noteworthy that the sequence of 20-bp repeats from the human distal part of the pJE clone displays a high level of identity with some markers with variable numbers of terminal r[r] ... See full document