[PDF] Top 20 Sequence-specific binding of DNA by the Moloney murine leukemia virus integrase protein.

... Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.. Affinity purifica[r] ... See full document

... retroviral DNA may affect multiple steps of the virus life cycle, suggesting that the IN protein may have other functions in addition to its integration ...immunodeficiency virus type 1 IN ... See full document

... genomic DNA were shown to be integrated within 1 to 2 bp of the site of initiation of transcription of tRNA genes ...as specific targets for Ty3 integration ... See full document

... IN virus was not due to reversion or second-site mutations, low-molecular-weight viral DNA was isolated from infected cells (22), and the full IN coding region was amplified by PCR (data not ...acid ... See full document

... the protein and DNA components were tested and found to profoundly affect the yield and distribution of the integration ...two binding sites, one for the LTR (the L site) and the other for the target ... See full document

... Pfu DNA polymerase ...The DNA oligonucleotide corresponding to K227A, oligonucleotide 2076 (5 9 -GAACCATCGCGGAGACT TTAAC-3 9 ), generates a BstU1 site (underlined) that allowed screening for the mutation by ... See full document

... with specific functions. The nucleo- capsid (NC) protein is a small, basic, carboxy-terminal portion of the Gag protein of most ...immunodeficiency virus type 1 (HIV-1) and Moloney ... See full document

... (Env) protein of Moloney murine leukemia virus is the primary mediator of viral ...the virus life cycle: (i) the expression and delivery of the Env protein to the cell ... See full document

... Fine-structure analysis of the linear double-stranded viral DNA indicated that all seven lethal alterations within the IN protein blocked the formation of the 3' recessed termini that no[r] ... See full document

... fusion protein, 100 nM synthetic viral DNA end oligonucleotide A194/A193, and competitor molecules in various molar or mass ratios in the standard integration reaction ...viral DNA end ... See full document

... viral DNA upon entry into cells. Cells inoculated with mutant virus particles were lysed to make Hirt ...Hirt DNA. WT1 and WT2 are WT virus particles used as positive controls for the ...of ... See full document

... EDTA-renatured protein sufficed, and yet treatment with NEM inactivated the EDTA- renatured ...the protein scaffold, thereby asso- ciating with the DNA ... See full document

... as Moloney murine leukemia virus (M-MuLV), differ from transforming viruses in their mechanisms of tumor ...the leukemia retroviruses, lacking oncogenes, employ other mechanisms, ... See full document

... fusion protein lysate, depending on expression levels, in a total volume of 200 μl was mixed with 200 μl of pre-equil- ibrated amylose resin (prepared according to manufactur- ers instructions, New England ... See full document

... target DNA junctions (single and double disintegrations) and an intramolecular transesterification product (foldback) that results from nucleophilic attack of a substrate oligonucleotide’s 39-OH on its own ... See full document

... envelope protein sequences that are involved in binding to the ecotropic retrovirus receptor ...for specific interaction with ecoR must be contained within the first 237 amino-terminal amino acids, ... See full document

... NC protein that are neces- sary for genome packaging contribute to 7SL recruitment, the 7SL content of virions produced by NC mutants was examined ...packaging, virus-producing cells were treated with ... See full document

... evident sequence homology either to each other or to the Int promoter element, and it therefore remains unclear how a DNA target for Bel-1 is defined at the primary sequence ...a ... See full document

... minus-strand DNA synthesis, since plus-strand DNA is made by using the minus-strand as a tem- plate, the resulting double-stranded viral DNAs will encode recombinant JZ481 on both ...recombinant ... See full document

... To determine whether the region was involved in the observed differential recognition, the Cys residues of M-MuLV IN were modified with NEM and assayed for the endonuclease activity of d[r] ... See full document