[PDF] Top 20 Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2.

... Comparative peptide analysis of HSV1 and HSV-2 showed that one polypeptide NC-7, 12,000 molecular weight had an identical methionine peptide profile and a very similar arginine peptide p[r] ... See full document

... First, the protein undergoes posttranslational processing in HSV-2-infected cells from a lower-molecularweight precursor pgD51 to a higher-molecularweight product gD56 in a manner which [r] ... See full document

... the structural differences between gC-1 and gC-2. In gC-1, a major part of the HS-binding domain was localized around the base of a small amino-terminal Cys127-Cys144 loop (39) and was found ... See full document

... statistical analysis of the genome isomer ...the analysis of the distribution of the four HSV-1 isomers, the method of extraction was improved, reaching ⬃70 to 80 kb as an average fiber length for ... See full document

... the analysis of structural ...the capsid. In high-resolution studies of the capsid shell, Zhou et ...distinct types of triplex based on the type of surrounding capsomers were de- ... See full document

... detailed structural information available on her- pesvirus capsids has emerged from cryoelectron microscopic reconstructions, initially of herpes simplex virus type 1 (HSV-1) ... See full document

... of herpes simplex virus type 1 (HSV-1) encodes a deoxyribonuclease that is frequently referred to as alkaline nuclease (AN) because of its high pH ...the structural ... See full document

... A recent study of the structural polypeptides specified by 53 different HSV-1 strains revealed that certain polypeptide bands with characteristic electrophoretic mobilities in SDS gels w[r] ... See full document

... the virus stocks. A single fluorescent plaque of K26GFP-1 is shown in ...blot analysis was performed on DNA extracted from both KOS- and K26GFP-infected cells to confirm the genotypes of the ... See full document

... and structural features of these interactive domains (Table ...cytomegalo- virus (HCMV) or Kaposi’s sarcoma-associated herpesvirus (KSHV) and tested these constructs in the yeast two-hybrid assay for ... See full document

... with 2% uranyl acetate (Canemco and Marivac), washed in distilled water, and dried on filter ...for 1 h in blocking buffer (PBS, 2% BSA, 0.2% gelatin) and then for 1 h with primary anti- ... See full document

... Analysis of crossover points in the recombinant Bxl28, using restriction endonucleases EcoRI, Bgl II, Hpa I, HindIII plus Hpa I, and Kpn I plus Xba L HSV-1 physical maps are shown above,[r] ... See full document

... A total of 122 clinical isolates of herpes simplex virus HSV from 107 patients typed by using an indirect immunoperoxidase technique with commercially available type-specific rabbit anti[r] ... See full document

... to herpes B virus were found to show cross-reactivity with HSV-1, whereas only 5 showed cross-reactivity with ...that herpes B virus has more antigenic cross-reactivity with ... See full document

... 1125 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Polyvalent rabbit antisera against herpes simplex virus type 1 and 2 HSV-1 and HSV-2, cytomegalovirus CMV, and Epst[r] ... See full document

... Virus stocks were prepared as described previously 13, except that viruses were grown in monolay140 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Transformation of mo[r] ... See full document

... When the mice were inoculated with each virus alone in their rear footpads, no mice were killed even after infection with high doses of viruses >106 PFU per mouse, whereas 100% of the mi[r] ... See full document

... Characterization and Composition of Multiple Capsid Forms of Subtypes 1 and 2 WADE GIBSON AND BERNARD ROIZMAN Department of Microbiology, The University of Chicago, Chicago, Illinois 606[r] ... See full document

... Sephadex G50 gel filtration chromatography of glycopeptides obtained by Pronase digestion of: a a total lysate of uninfected cells labeled with D- ["Clglucosamine from 4 to 12 h after mo[r] ... See full document

... Evidence that most of the ICP we have identi0 fied are specified by viral genetic information is -W based on three types of experiments dealing with comparisons of polypeptides synthesiz[r] ... See full document