[PDF] Top 20 A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution

... Single-stranded DNA binding proteins (SSBs) play an important role in DNA processing events such as replication, recombination and re- ...single-stranded DNA binding pro- tein 1 ... See full document

... 1% bovine serum albumin) was incubated with endonuclease (Benzonase; 50 U/ml; Sigma) at 37°C for 10 min followed by an extensive wash with buffer B. The immobilized MBP-HIVIN was incubated with MAb 2-19 or MAb 8-16 on ... See full document

... Fig. 5B shows the absorption spectrum of complex 2, showing an absorption band at λ max = 289 nm due to π–π electronic transition of the aromatic moiety in the complex and a weak absorption peak at 510 nm due to d–d ... See full document

... ChIP-qPCR analysis allowed us to more directly characterise the effect of CTD expression upon ParB spreading ...specific binding, because the PCR product at parS is much larger than the 16 bp parS site or ... See full document

... In smFRET experiments involving nucleic acids and nucleic- acid-binding proteins, fluorophore-labeled nucleic acids and pro- teins can be immobilized on a surface, extending imaging lifetimes. The fluorophores are ... See full document

... without DNA. In contrast, the basic region in bZIPs lack an ancillary DNA-binding motif is ...bind DNA as monomers with the basic region forming a helix only transiently in ...DNA ... See full document

... microarray analysis at The Scripps Research Institute microarray ...comparison analysis, 1R-Chl treated ...pathway analysis, MIT Board pathway library was ... See full document

... The N-terminus of ␣9 pushes the ␣10 helix aside, stretching the adjacent segment (residues 412–417, domain 2A) and breaking the hydrogen bond between the side chain of K414 and the main chain carboxyl of G416 ... See full document

... single-stranded DNA agarose chromatography by using a modification of the method of Hang et ...single-stranded DNA agarose column in 800 mM to 1 M NaCl, resulting in 80 to 90% purification of LEF-3 ... See full document

... Diffraction data were recorded for two crystal forms (table 1) at the Diamond Light Source, respectively. Initial phases were determined based on an iodine derivative, using single-wave- length anomalous diffraction data ... See full document

... broken DNA ends together and recruiting ATM which facilitates ATM activation ...of hSSB1 to DSBs we explored the possibility that hSSB1 foci formation is dependent on the classical DNA damage ... See full document

... and binding kinetics of PA·dsDNA interactions where the N-terminal heterocycle is systematically altered ...impact DNA binding affinity for its target sequence, NMR structural analysis ... See full document

... and DNA process- ing enzyme that has roles in genome ...explore structural events along the chemical reaction coordinate of ATP hy- drolysis at an unprecedented level of ...ssDNA binding channel in ... See full document

... repetitive DNA may itself be a causative factor in mutagenesis ...the DNA synthesis machinery in replicating through this type of ...reiterative DNA synthesis or replication restart via recombination ... See full document

... 21 structural proteins in the virion of Spodoptera frugiperda ascovirus 1a (SfAV1a), a virus with a large, double-stranded DNA genome of 157 kbp, which attacks species of the lepidopteran family ... See full document

... is limited to the zone of adiabatic stabilization. Further saturation is possible only in the case of a water-am- monia solution with heat rejection. Thus the heat re- jection in the inverse rectificator should ... See full document

... transcription factor, pericarp colour (P I), are epigenet ical ly regulated (Cocciolone et al., 200 1 ). P l -wr (white pericarp / red cob) and p l -rr (red pericarp / red cob) are two alleles determining two different ... See full document

... classical binding theory, the color yellow is formed in consciousness by first making a mechan- ical connection between the three locations in the yellow panel of Figure ... See full document

... With the exception of our previously solved wild-type 09N1 structure, all solved group 1 uncomplexed native structures (N1, N4, N5, and N8) have a 150-cavity, and all solved group 2 uncom- plexed structures (N2, N3, N4, ... See full document

... As first demonstrated in 1986, laser light that is focused to a diffraction-limited ‘point’ can act as a handle to manipulate dielectric particles (37). In a conventional point trap, the force exerted on the trapped ... See full document