[PDF] Top 20 Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... the UL25 protein ...the protein was purified to near homogeneity using a glutathione-Sepharose ...conversion from the expected 57-kDa protein to a new, 48-kDa ...of UL25 was ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 UL25 DNA Packaging Protein Reveals Regions That Are Important after the Viral DNA Has Been Packaged
... The herpes simplex virus type 1 (HSV-1) UL25 gene encodes a minor capsid protein, pUL25, that is essential for packaging the full-length viral ...addition, ... See full document
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The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential ...the UL25 gene product is a virion ... See full document
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Cloning and characterization of herpes simplex virus type 1 oriL: comparison of replication and protein-DNA complex formation by oriL and oriS.
... functional characterization of HSV-1 origins has focused almost exclusively on oriS since the large oriL palin- drome is deleted upon cloning in bacteria (6, 24, 30, ...HSV-1 DNA from ... See full document
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The Herpes Simplex Virus Type 1 Cleavage/Packaging Protein, UL32, Is Involved in Efficient Localization of Capsids to Replication Compartments
... wild-type virus and a virus with a mutant version of cleav- age and packaging gene UL6, capsids localize exclusively to replication compartments, cells infected with the UL32 mutant exhibit an ... See full document
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Structural studies of a uracil-DNA glycosylase from herpes simplex virus type 1
... Thin plate clusters are another morphology that occurs with the protein/ trimeric thymidine DNA mixture. With dimeric thymidine (Sigma Chemical Company Ltd), the plates are the favoured morphology (Figure ... See full document
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Packaging Determinants in the UL11 Tegument Protein of Herpes Simplex Virus Type 1
... for virus assembly and budding (12). Although HSV-1 has not been reported to as- semble on detergent-resistant, lipid-raft domains, it is possible that palmitylation enables UL11 to target specifically to ... See full document
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The Herpes Simplex Virus Type 1 DNA Packaging Protein UL17 Is a Virion Protein That Is Present in Both the Capsid and the Tegument Compartments
... infectious virus. In the absence of DNA packaging, the internal scaffolding proteins are still cleaved but remain trapped inside the angularized capsid, generating a dead-end product, the B ... See full document
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document
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The UL25 Gene Product of Herpes Simplex Virus Type 1 Is Involved in Uncoating of the Viral Genome
... viral DNA at the ...after virus adsorption for 1 h at the PT contained fewer capsids in the cell nuclei than wt HSV-1-infected cells, and DNA-con- taining capsids were not observed, ... See full document
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Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids
... The herpes simplex virus 1 (HSV-1) virion consists of a 152-kbp double-stranded DNA (dsDNA) genome that is en- closed in an icosahedral capsid, which is itself surrounded by an ... See full document
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Branched structures in the intracellular DNA of herpes simplex virus type 1.
... for packaging of mature, linear ...stein-Barr virus (30). Alkaline nuclease has been found in a protein complex bound to the cleavage site of the HSV-1 ge- nome ...its DNA to nearly ... See full document
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Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1.
... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... viral DNA (26, ...viral DNA packaging signal (3), a property shared with the homologous protein of human cytomegalovirus (CMV), UL56 ...which DNA is inserted into the capsid (22, 23, ... See full document
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Packaging of Genomic and Amplicon DNA by the Herpes Simplex Virus Type 1 UL25-Null Mutant KUL25NS
... The herpes simplex virus type 1 (HSV-1) mutant KUL25NS, containing a null mutation within the UL25 gene, was isolated and characterized by McNab and coworkers ...viral ... See full document
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... UL17-stop virus contains a 16-bp insertion at position 32718 in the HSV-1 strain 17 genome, with stop codons in all three reading frames, truncating the UL17 open reading frame after codon 260 ...with ... See full document
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Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... of UL25 structure. The crystal structure of UL25 (amino acids 134 to 580) has been determined ...The UL25 protein consists of numerous flexible loops extend- ing from a rigid core ... See full document
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Herpes Simplex Virus Capsid Structure: DNA Packaging Protein UL25 Is Located on the External Surface of the Capsid near the Vertices
... of UL25 from insect cells was carried out beginning with Sf9 cells infected with ...removed from the lysate by centrifugation at 30,000 rpm in a Beckman TL-100 ultracen- trifuge (TLA rotor) for 30 ... See full document
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Role of the UL25 Gene Product in Packaging DNA into the Herpes Simplex Virus Capsid: Location of UL25 Product in the Capsid and Demonstration that It Binds DNA
... UL25 protein is part of the penton and the hexon structures. If the HSV-1 capsid vertex (penton) serves as the site for DNA entry, one of the DNA-packaging proteins, UL25, ... See full document
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Characterization of two conformational forms of the major DNA-binding protein encoded by herpes simplex virus 1.
... SPANG Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115 We have resolved two electrophoretic species of the major DNA-binding protein[r] ... See full document
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