[PDF] Top 20 Structural Features of Nectin-2 (HveB) Required for Herpes Simplex Virus Entry

... of herpes simplex virus (HSV) entry into cells is the binding of viral glycoprotein D (gD) to a cellular ...Human nectin-2 (also known as HveB and Prr2), a member of the ... See full document

... the nectin-1- ⌬ 2 protein through the exocytic pathway, we studied its electrophoretic mobility after endoglycosidase ...F. Nectin-1 and nectin-1- ⌬ 2, tagged at the C terminus with an ... See full document

... HSV-1 entry receptors in HIS ...receptor nectin-1 was evident in HIS cells, while levels of HVEM and 3-OST-3 were found to be insignificant, suggesting that it is nectin-1 that is the gD receptor ... See full document

... the entry of many viruses, includ- ing the alphaherpesviruses (2, 4, ...are required for HSV membrane fusion ...individual entry events may include me- diating appropriate membrane rigidity ... See full document

... of virus infection by HSV requires, as a prerequisite, the specific interactions between viral surface glycoproteins and the host cellular receptors ...multiple virus envelope proteins other than gD have ... See full document

... B virus (BV) (or macacine herpesvirus 1, herpesvirus simiae, monkey B virus, or cercopith- ecine herpesvirus 1) and cercopithecine herpesvirus 2 (CeHV-2) (or simian agent 8) are primate ... See full document

... HSV entry by either endocytic or nonendocytic routes are rapid (12, 31, 54, ...CHO- nectin-1 cells is necessarily delayed (t 1/2 of ⬃ 30 min) ...the entry pathway taken, incoming capsids dock ... See full document

... of nectin-1 in CD4 and in the poliovirus receptor have been shown to be critical for interactions with human immu- nodeficiency virus and poliovirus, respectively (1, 4, 31, ...as virus receptors ... See full document

... syndrome virus by factor Xa is important for the entry of the severe acute respi- ratory syndrome virus (9), viral entry of Ebola virus requires endosomal proteolytic cleavage of its ... See full document

... gI, entry into T cells is distinct in that neither gE nor gI is ...HSV entry, by either cell-free virus or cell-cell spread between epithelial cells (gB, gD, gH, and gL), is required for ... See full document

... d301 virus (14) contains a deletion in the ICP8 ...is required for viral DNA replication and late gene (g2) ...n504 virus contains a nonsense mutation in the ICP27 gene ...is required for ... See full document

... 31-68) virus caused cell fusion in the gK-complementing cell line VK302, while the HSV-1(F) gK ⌬ 31-68 virus did not ...117 virus was efficiently complemented by VK302 cells (22), while the ... See full document

... pH-dependent entry pathway are not ...HSV entry (2). Entry of gE ⫺ , gG ⫺ , gI ⫺ , gJ ⫺ , gM ⫺ , and Us9 ⫺ mutants was blocked by ammonium chloride in a concen- tration-dependent manner, ... See full document

... HSV-1 entry has been observed from either cell surface (35, 44) or with a pref- erence for the basolateral surface (19, 26, 34, ...the virus and the cell mem- brane at the two surfaces is ...to ... See full document

... higher virus-induced cell fusion than the gB ⌬ 28 mutation, most likely because this virus spread more rapidly than the gB⌬28 ...on virus replication, viral titers were obtained at 24 or 48 h after ... See full document

... HSV entry (38, ...herpesvirus entry mediator (HVEM), that can enhance the susceptibility of both of these cell types to infection by a variety of HSV strains ...cellular entry factors required ... See full document

... Herpes simplex virus 1 (HSV-1) is a ubiquitous human pathogen that enters cells by the receptor-mediated fusion of the viral envelope with a host cell ...for entry to take ...herpesvirus ... See full document

... for 2 h and then were incubated with soluble forms of HSV-1 gD and PRV gD (gD:Fc hybrid molecules), followed by fixation and addition of labeled anti-Fc ...Figure 2 shows that both the HSV-1 and PRV gD:Fcs ... See full document

... is required for global folding of the entire ...disease virus (MDV) this residue is a Y and in the other members it is bulky and hydrophobic, within the sequence FGAAADTYADMF (where bold indi- cates very ... See full document

... surface nectin-1 early in infection. Attachment of virus to the cell surface is mediated primarily by gC and gB bound to heparan sulfate proteoglycans (18, 27, ...on nectin-1 was not accessible when ... See full document