[PDF] Top 20 Using Ion Mobility Spectrometry to Study Protein Conformations in the Gas Phase

... conducted using identical ...window. Using such calibration data for analytes with large td would require a long extrapolation (dotted line in Figure ... See full document

... initial conformations was observed for ConA. This suggests that the gas phase longevity of the native structures is proportional to the ion mass and, most likely, inversely proportional to its ... See full document

... core protein of hepatitis B virus is important for a full understanding of the viral life cycle and the development of novel therapeutic ...core protein (CP149) comprises the capsid assembly domain, and the ... See full document

... the gas phase (Ehn et ...oxidized gas-phase species are present at low ambient concentrations (ppt levels), but they can condense onto particles and be a significant contribution to particle ... See full document

... compounds using gas phase motilities of ions in weak eclectic fields at ambient ...The mobility of an ion is determined by the ion velocity that is measured in the drift tube of ... See full document

... disorder. Ion mobility spectrometry, in combination with mass spectrometry (IM-MS), offers a rapid and sensitive means of probing ensembles of protein structures through measurement of ... See full document

... Synthetic molecules including metal-ring host-guest complexes, rotaxanes, catenanes, macrocyclic porphyrin-like systems and synthetic baskets like resorcinarenes represent an exciting branch of chemistry due to their ... See full document

... kinetic and thermodynamic stability. At increased collision energies, ion collisions with gas within the collision cell cause the protein to unfold. Between 25 and 40 V, a number of coexisting ... See full document

... mass spectrometry (MS) is a commonly used analytical technique for probing protein structure and ...while protein molecules remain solvated in the droplet ...that protein ions formed during ... See full document

... this study, we present the first demonstration of the ion mobility mass spectrometry (IMS-MS) interfaced with an electrospray ionization (ESI) source that enables the molecu- lar ... See full document

... 5+ ion is the lowest and most native-like charge state for which the compaction phenomenon is observed via IM-MS, we will focus the rest of our analysis on this ...previous study on cytochrome c-CE ... See full document

... the ion mobility ...calibrated using 30 µM caesium iodide (Sigma Aldrich, ...spectrometer using gold coated borosilicate capillaries prepared in-house ...The gas in the T-Wave IM ... See full document

... The preceding considerations refer to traditional drift tube IM-MS. However, many commercial instruments (including the one used for the current work) employ travelling wave voltages with nitrogen as buffer gas. A ... See full document

... when using different sets of calibrants, or different sets of values for the same ...different ion structures), one must first know the uncertainty associated with the calibrant ... See full document

... A complication is that seedcotton released va- pors that showed response in the same region of the mobility spectrum as that of plastics. Though the response was lower on a mass basis than that for plastic, it is ... See full document

... IM for the separation, identification, and structural characterization of analytes across diverse fields of science. These include: ionic clusters (Bowers et al., 1993; Von Helden et al., 1993; Dugourd et al., 1998; ... See full document

... spatial resolution and analyte specificity. Several groups have presented data on xenobiotic distribution within whole body sections since the first report by Rohner et al., 2005 and they showed the distribution of an ... See full document

... portable. Gas Chromatography and Mass Spectrometry are the most commonly applied technologies in the VOCs detection ...Traditional Gas Chromatography achieved VOCs detection at ppm level with the ... See full document

... While performing well on single spectra, there is room for improvement in merging one-dimensional peak mod- els into two-dimensional peak models. Our method has to be further evaluated in clinical studies or ... See full document

... Due to the different types of sensors deployed within the instrument, it is useful to transduce each of the sensor outputs into a common form—in this case voltage. The six metal-oxide sensors were driven with a constant ... See full document