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fluorescence microscopy

Super resolution fluorescence microscopy studies of human immunodeficiency virus

Super resolution fluorescence microscopy studies of human immunodeficiency virus

... Super-resolution fluorescence microscopy studies and their contribution to the understanding of HIV-1 replication cycle (illustrated in the lower ...their fluorescence intensity line profiles (lower ...

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SPOT DETECTION METHODS IN FLUORESCENCE MICROSCOPY IMAGING: A REVIEW

SPOT DETECTION METHODS IN FLUORESCENCE MICROSCOPY IMAGING: A REVIEW

...  Accurate spot detection: the images obtained via fluorescence microscopy imaging may be blurred and noisy which makes it difficult to analyse them. The presence of noise in these images limits the per- ...

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Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon

Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon

... conventional fluorescence micro- ...conventional fluorescence microscopy or an inter- ference filter system ...light microscopy (LM) of Giemsa-stained thick blood smears were compared to the ...

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3D Super-Resolution Fluorescence Microscopy Using Cylindrical Vector Beams

3D Super-Resolution Fluorescence Microscopy Using Cylindrical Vector Beams

... this microscopy system can theoretically generate an arbitrarily small 3D spot, if only the saturation factor γ is sufficiently ...the fluorescence spot size as a function of γ ...STED fluorescence ...

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Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy

Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy

... Results: Live-cell imaging with fluorescent asbestos probes was successfully utilized to dynamically analyze asbestos phagocytosis. The fluorescently labeled asbestos fibers were phagocytosed by RAW 264.7 macrophages. ...

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Dyke Award  Europium DTPA: a gadolinium analogue traceable by fluorescence microscopy

Dyke Award Europium DTPA: a gadolinium analogue traceable by fluorescence microscopy

... These soaked tissue specimens were then used to calibrate and establish sensitivities for the detection of Eu and Gd by fluorescence microscopy and electron probe microanalysis.. A secon[r] ...

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Superresolution fluorescence microscopy for 3D reconstruction of thick samples

Superresolution fluorescence microscopy for 3D reconstruction of thick samples

... superresolution fluorescence microscopy based on single-molecule localization has been just a few micrometers, this is a significant improvement which is expected to open a way to wide application of ...

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Measuring DNA content in live cells by fluorescence microscopy

Measuring DNA content in live cells by fluorescence microscopy

... data collected from flow cytometry. Therefore, we per- formed a side-by-side experiment comparing asynchro- nous cell cycle distributions acquired in live cells by fluorescence microscopy to that of fixed ...

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Total internal reflection fluorescence microscopy for characterizing biochemical interactions

Total internal reflection fluorescence microscopy for characterizing biochemical interactions

... traditional fluorescence microscopy, precise axial tracking, applicability to multi-color and multi-photon excitation, and the ability to image cells longer due to higher survival rates achieved by using ...

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HIV taken by STORM: Super resolution fluorescence microscopy of a viral infection

HIV taken by STORM: Super resolution fluorescence microscopy of a viral infection

... We used dSTORM to visualize matrix and capsid pro- teins in cell-free HIV-1 virions and in infected lymphoid cells. Similar to previously describe [7,14], we first gener- ated HIV-1 particles that contained the green ...

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Multi-class image segmentation in fluorescence microscopy using polytrees

Multi-class image segmentation in fluorescence microscopy using polytrees

... Abstract. Multi-class segmentation is a crucial step in cell image analy- sis. This process becomes challenging when little information is available for recognising cells from the background, due to their poor ...

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Multi-class Image Segmentation in Fluorescence Microscopy Using Polytrees

Multi-class Image Segmentation in Fluorescence Microscopy Using Polytrees

... Abstract. Multi-class segmentation is a crucial step in cell image analy- sis. This process becomes challenging when little information is available for recognising cells from the background, due to their poor ...

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Bayesian multi-target tracking: application to total internal reflection fluorescence microscopy

Bayesian multi-target tracking: application to total internal reflection fluorescence microscopy

... of fluorescence microscopy is painstakingly slow and suffers from poor accuracy and ...synthetic fluorescence microscopy sequences through simulation of the image formation pro- cess and ...

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Quantification of confocal fluorescence microscopy for the detection of cervical intraepithelial neoplasia

Quantification of confocal fluorescence microscopy for the detection of cervical intraepithelial neoplasia

... Acriflavine fluorescence stain for 2 min while shaking on a shakerat low speed, as previously described ...cal fluorescence microscopy was performed using a bench-top Carl Zeiss Axio Imager Z1 ...

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Three photon light sheet fluorescence microscopy

Three photon light sheet fluorescence microscopy

... light-sheet fluorescence microscopy (LSFM) has revolutionized the field of imaging by using a thin sheet of light to optically section samples which are typically ...

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Fluorescence microscopy methods for the determination of somatic cell count in raw cow’s milk

Fluorescence microscopy methods for the determination of somatic cell count in raw cow’s milk

... direct fluorescence microscopy method with ethidium bromide staining can be used for somatic cell counting in raw cow’s ...using fluorescence microscopic methods and flow ...direct ...

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Principles of Microscopy and Confocal and Fluorescence Microscopy

Principles of Microscopy and Confocal and Fluorescence Microscopy

... Light Microscopy follows the series of successful courses in Light Microscopy, Confocal and Fluorescence Microscopy and Electron ...in microscopy, and for people who use microscopes in ...

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Feasibility of magnetic bead technology for concentration of mycobacteria in sputum prior to fluorescence microscopy

Feasibility of magnetic bead technology for concentration of mycobacteria in sputum prior to fluorescence microscopy

... This study evaluated the feasibility of using an early prototype ligand-coated magnetic bead technology to concentrate M. tuberculosis prior to detection by LED- based fluorescence microscopy (magnetic bead ...

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Fluorescence Optofluidic Microscopy and Fluorescence Microscopy Based on the Talbot Effect

Fluorescence Optofluidic Microscopy and Fluorescence Microscopy Based on the Talbot Effect

... chip fluorescence microscope system, termed fluorescence optofluidic microscope ...of fluorescence microscopy imaging of samples in fluid ...the fluorescence emissions are collected by ...

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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

... of 22mW from both systems directed into the scanhead, Figure 4A shows the images obtained with the PCF-enabled source (a) and the conventional Ti:Sapphire laser (b) at a grating spacing of 14 mm. This 2 mm increase in ...

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