genome multilocus sequence typing (wgMLST)
Whole Genome Multilocus Sequence Typing of Extended Spectrum Beta Lactamase Producing Enterobacteriaceae
... Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identifi- cation of sources and routes of transmission in outbreak settings, but current methods ...
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Epidemiological Typing of Serratia marcescens Isolates by Whole Genome Multilocus Sequence Typing
... is genome sequencing. The use of whole-genome (draft) sequences (WGS) to compare bacterial isolates in detail, and to delineate their spread, is based on either the detection of single nucleotide variants ...
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Hash Based Core Genome Multilocus Sequence Typing for Clostridium difficile
... core genome multilocus sequence typing (cgMLST) in which alleles at each gene are reproducibly converted to a unique hash, or short string of letters ...replicate sequence pairs using ...
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Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Genome Wide Typing of Clostridium difficile
... and typing nomenclature are missing. Here we report a core genome multilocus sequence typing (cgMLST) approach developed for ...MLST sequence types with Bayesian inference ...the ...
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Core Genome Multilocus Sequence Typing: a Standardized Approach for Molecular Typing of Mycoplasma gallisepticum
... on sequence analysis of 5 loci remains insufficient for accurate outbreak ...massive sequence data and the diverse properties of different genes within bacterial genomes results in a lack of standard ...
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Core Genome Multilocus Sequence Typing Scheme for High Resolution Typing of Enterococcus faecium
... other typing methods, the thresholds and criteria formulated for the ...other typing methods, cgMLST-based criteria or thresholds help stratify and refine data by providing more sensitive and specific ...
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Phylogenetic Analysis of Mycobacterium tuberculosis Strains in Wales by Use of Core Genome Multilocus Sequence Typing To Analyze Whole Genome Sequencing Data
... whole- genome sequencing (WGS) has been a barrier to its widespread use in tuberculosis ...nome multilocus sequence typing (cgMLST) analysis of whole-genome sequencing ...
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Culture of Clinical Specimens Reveals Extensive Diversity of Legionella pneumophila Strains in Arizona
... Raw sequence reads were analyzed using an L. pneumophila whole- genome multilocus sequence typing (wgMLST) method that is based on sequence variation present at a set of ...
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A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis
... ence genome for downstream analyses of variant-calling investigations in order to produce reliable results, by applying the most reasonable method for data evaluation at a given time and for a certain strain ...
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Defining a Core Genome Multilocus Sequence Typing Scheme for the Global Epidemiology of Vibrio parahaemolyticus
... locus sequence typing (MLST) database for ...core genome MLST (cgMLST) analysis as one of the most straightforward ways to explore complex genomic data in an epidemiological con- ...the genome ...
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Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Whole Genome Sequence Based Typing of Listeria monocytogenes
... MLST and cgMLST both use alleles and not nucleotide poly- morphisms as units of comparison. Irrespective of the number of nucleotide polymorphisms involved, each allelic change is num- bered as a single event; i.e., an ...
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Determination of Accessory Gene Patterns Predicts the Same Relatedness among Strains of Streptococcus pneumoniae as Sequencing of Housekeeping Genes Does and Represents a Novel Approach in Molecular Epidemiology
... core genome (multilocus sequence typing [MLST]), but these do not provide informa- tion on gene content that may affect the potential of isolates to cause invasive pneumococcal ...30 ...
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Multilocus Sequence Typing of Corynebacterium ulcerans Provides Evidence for Zoonotic Transmission and for Increased Prevalence of Certain Sequence Types among Toxigenic Strains
... Locus amplification and sequencing. Each PCR was carried out in a 50- l total volume using HotStarTaq Master Mix (Qiagen). Sequences of the primers and the expected amplicon sizes are given in Table 1. Primers for ...
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Multilocus Sequence Typing of Total Genome Sequenced Bacteria
... species, multilocus sequence typing (MLST) is considered the “gold standard” of typing, but it is traditionally performed in an expensive and time-consuming man- ...short sequence reads ...
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Multilocus Sequence Typing System for Group B Streptococcus
... the genome sequence of the GBS strain NEM316 (11) with homologous sequences from other ...and sequence diversity observed in pilot studies with a restricted set of GBS ...
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Multilocus Sequence Typing of Mycobacterium xenopi
... electrophoresis-based typing methods, suffers from poor interlab reproducibility and portabil- ...contrast, multilocus sequence typing (MLST) is readily por- table and ...
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Prevalence and Sequence Diversity of a Factor Required for Actin Based Motility in Natural Populations of Burkholderia Species
... and multilocus sequencing typing (MLST) (8), comparison of complete genome sequences (12, 18), and mi- croarray analysis of gene content and transcription (15, 21) indicate that Burkholderia mallei ...
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Comparing Pulsed Field Gel Electrophoresis with Multilocus Sequence Typing, spa Typing, Staphylococcal Cassette Chromosome mec (SCCmec) Typing, and PCR for Panton Valentine Leukocidin, arcA, and opp3 in Methicillin Resistant Staphylococcus aureus Isolates at a U S Medical Center
... lates may be whole-genome sequencing. Recently, five studies have demonstrated the power of whole-genome sequencing to compare strains of S. aureus by identifying single-nucleotide poly- morphism profiles ...
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Multilocus Sequence Typing System forCampylobacter jejuni
... Choice of loci. A number of candidate loci, encoding enzymes responsible for intermediary metabolism, were identified by searching the C. jejuni genome database (http:www.sanger.ac.uk/Projects/C_jejuni/) (26) with ...
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Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae
... molecular typing of ...molecular typing targeted the gene en- coding the major surface protein (P1) of ...molecular typing and have resulted in the identi- fication of an additional subtype and three ...
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