Multilocus variable-number tandem repeat analysis
Molecular typing of Salmonella enterica serovar Enteritidis isolates from food-producing animals in Japan by multilocus variable-number tandem repeat analysis: evidence of clonal dissemination and replacement
... Background: Salmonella enterica serovar Enteritidis is a zoonotic pathogen. Human infections are associated with contaminated eggs and egg products. In Japan, since 1989, the incidence of food-borne disease caused by S. ...
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Comparison of Multilocus Variable Number Tandem Repeat Analysis and Whole Genome Sequencing for Investigation of Clostridium difficile Transmission
... However, multilocus variable-number tandem-repeat analysis (MLVA) (3–5) and whole-genome sequencing (WGS) of ...copy number at ⱖ 7 tandem- repeat loci to ...
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Comparison of Multilocus Variable-Number Tandem-Repeat Analysis and Whole-Genome Sequencing for Investigation of Clostridium difficile Transmission
... However, multilocus variable-number tandem-repeat analysis (MLVA) (3–5) and whole-genome sequencing (WGS) of ...copy number at ⱖ 7 tandem- repeat loci to ...
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Multilocus Variable Number Tandem Repeat Analysis and Multilocus Sequence Typing Reveal Genetic Relationships among Clostridium difficile Isolates Genotyped by Restriction Endonuclease Analysis
... study, multilocus sequence typing (MLST) and multilocus variable-number tandem-repeat analysis (MLVA) were performed on a set of 157 spatially and temporally diverse ...
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Evaluation of a Multilocus Variable Number Tandem Repeat Analysis Scheme for Typing Human Brucella Isolates in a Region of Brucellosis Endemicity
... Recently, multilocus variable- number tandem-repeat analysis (MLVA) was found to have a high degree of resolution when it was applied to collections of Brucella isolates from ...
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Use of Multilocus Variable Number Tandem Repeat Analysis for Typing Mycobacterium avium subsp paratuberculosis
... reservoirs, multilocus variable-number tandem-repeat analysis (MLVA) was explored and compared to IS900 restriction fragment length polymorphism (RFLP) ...
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Multilocus Variable Number Tandem Repeat Analysis of Clostridioides difficile Clusters in Ribotype 027 Isolates and Lack of Association with Clinical Outcomes
... ABSTRACT The epidemiology of Clostridioides difficile infection (CDI) has drastically changed since the emergence of the epidemic strain BI/NAP1/027, also known as ri- botype 027 (R027). However, the relationship between ...
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Quantification of Clostridioides (Clostridium) difficile in feces of calves of different age and determination of predominant Clostridioides difficile ribotype 033 relatedness and transmission between family dairy farms using multilocus variable-number tandem-repeat analysis
... using multilocus variable-number tandem-repeat analysis (MLVA) to assess intra- and between-farm ...the number of calves and the farm ...the number of ...
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Multilocus Variable Number Tandem Repeat Analysis of Mycoplasma pneumoniae Clinical Isolates from 1962 to the Present: a Retrospective Study
... (RFLP) analysis of the P1 gene (4, 18) and real-time PCR followed by high-resolution melt analysis (HRM) targeting the region of ...multiple-locus variable-number tan- dem-repeat (VNTR) ...
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High Resolution Genetic Fingerprinting of European Strains of Anaplasma phagocytophilum by Use of Multilocus Variable Number Tandem Repeat Analysis
... mony analysis of an alignment of these 19 sequences indicated that the strains examined in our study shared a close and specific evolutionary relationship with those previously exam- ined ...
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Characterization of Genetic Diversity of Bacillus anthracis in France by Using High Resolution Melting Assays and Multilocus Variable Number Tandem Repeat Analysis
... (HRM) analysis, we developed a cost-effective method to genotype a set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome of Bacillus ...Multiple-locus ...
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Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus: Evaluation of a New Multilocus Variable Number Tandem Repeat Analysis Typing Method
... into variable repeat regions that could evolve more rapidly by an introduction or deletion of a single repeat, clonal types might be affected by genomic rearrange- ments to different extents in the ...
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Multilocus Variable Number Tandem Repeat Analysis Distinguishes Outbreak and Sporadic Escherichia coli O157:H7 Isolates
... PCR amplification and sequence analysis of potential VNTRs. Initially, 11 loci of a subset of 16 PHIDL isolates were se- quenced to determine if the TR locus had sufficient variability (data not shown). If ...
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Clostridium botulinum Group I Strain Genotyping by 15 Locus Multilocus Variable Number Tandem Repeat Analysis
... Microbial Tandem Repeats database (http: ...accession number NC_009698), 57 potential VNTR loci were detected, including 10 pre- viously investigated by Macdonald and colleagues ...
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Utility of Multilocus Variable Number Tandem Repeat Analysis as a Molecular Tool for Phylogenetic Analysis of Shigella sonnei
... groups. Tandem-repeat loci may have variable num- bers in one species or serotype but may be invariable in ...limited number of common VNTR loci (unpublished ...
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Application of Multilocus Variable Number Tandem Repeat Analysis for Molecular Typing of the Agent of Leptospirosis
... Epidemiologic investigation of leptospirosis in New Cale- donia. We validated MLVA as a useful tool for typing Lepto- spira spp. by conducting a study in New Caledonia, a region of high endemicity. We subjected 156 ...
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Multilocus Variable Number Tandem Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals
... Stability of VNTR loci (study group A isolates). Serial iso- lates from individual patients that were cultured on different dates were subjected to MLVA to determine if the seven VNTR loci were sufficiently stable to ...
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Multilocus Variable Number Tandem Repeat Analysis Scheme for Chlamydia felis Genotyping: Comparison with Multilocus Sequence Typing
... coding tandem repeats identified in two putative proteins (CF0611 and CF0618) and the deletion in the hctB gene (CF0595) is ...Coding tandem repeats are believed to enhance spe- cific properties of ...
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Multilocus Variable Number Tandem Repeat Analysis for Molecular Typing of Shigella sonnei
... Genetic characterization of bacterial strains with a variety of genotyping methods is frequently applied for epidemiological investigation. A number of genotyping methods have been developed for S. sonnei (3, 15, ...
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Multilocus variable number tandem repeat analysis of clinical isolates of Aspergillus flavus from Iran reveals the first cases of Aspergillus minisclerotigenesassociated with human infection
... it was noted that in this collection there is a paucity of toxigenic isolates, with only five for the 54 (9.25%) clin- ical and environmental isolates testing positive for afla- toxin. This is very similar to a recent ...
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