Optimisation for Randomly amplified polymorphic DNA, (RAPD) peR
Randomly Amplified Polymorphic DNA Analysis ofErysipelothrix spp
... of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was ...
5
Identification and DNA fingerprinting of Legionella strains by randomly amplified polymorphic DNA analysis
... The randomly amplified polymorphic DNA (RAPD) technique was used in the development of a fingerprint- ing (typing) and identification protocol for Legionella ...
5
Characterization of Streptococcus agalactiae strains by randomly amplified polymorphic DNA analysis
... or amplified fragments that characterize the virulent clones would indicate a high risk of ...fragments amplified by the RAPD system that are characteristic of particular groups of strains which contain ...
7
Characterization of Scedosporium prolificans clinical isolates by randomly amplified polymorphic DNA analysis
... by randomly amplified polymorphic DNA (RAPD) analysis was used to differentiate Scedo- sporium prolificans ...detect DNA polymorphisms among 17 ...
5
Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates
... few polymorphic products within species in this ...The polymorphic pattern seen in some ...for DNA preparations, fastidi- ous spheroplasting, synthesis of probes, hybridizations, or film ...
9
Randomly Amplified Polymorphic DNA as a Valuable Tool for Epidemiological Studies of Paracoccidioides brasiliensis
... 2003 Randomly amplified polymorphic DNA (RAPD) has been successfully used to detect genetic variations among isolates of Paracoccidioides ...
6
Molecular Typing of Borrelia burgdorferiSensu Lato by Randomly Amplified Polymorphic DNA Fingerprinting Analysis
... To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n 5 49) with various clinical entities and from ticks (n 5 83) or dogs ...
9
Assessment of Genetic Diversity in Four Brassica Species using Randomly Amplified Polymorphic DNA Markers
... were polymorphic and hence the total polymorphism percentage was found to be ...produced per primer varied from 9 to 26 with a mean of ...bands per primer. The molecular size of the amplified ...
6
Randomly amplified polymorphic DNA PCR for comparison of Mycobacterium abscessus strains from nosocomial outbreaks
... purified DNA was relatively stable and produced sharper, clearer, and greater numbers of amplicon bands, we prefer to use purified DNA as the ...crude DNA extract treated by boiling could identify ...
8
Characterization of Streptococcus agalactiae Isolates of Bovine and Human Origin by Randomly Amplified Polymorphic DNA Analysis
... Received 21 May 1999/Returned for modification 19 July 1999/Accepted 9 September 1999 Streptococcus agalactiae is considered one of the major causes of bovine intramammary infections. It is also found in the vaginas of ...
8
Evaluation of Genetic Diversity of Pearly Mussel, Parreysia Corrugata by Randomly Amplified Polymorphic DNA (RAPD)
... MATERIAL AND METHODS P. corrugata were hand collected from five distantly located districts in Maharashtra viz. Thane, Ahmednagar, Ratnagiri, Aurangabad and Nanded, brought alive to Aquaculture laboratory, Department of ...
5
Achromobacter xylosoxidans Genomic Characterization and Correlation of Randomly Amplified Polymorphic DNA Profiles of Cystic Fibrosis Patients
... through DNA sequencing, the phenotypic traits that allow these bacteria to survive and to be at an advantage in these ...of DNA replication and repair, and the generation of variation allows adaptation to ...
6
Genetic Distance and Diversity in Table Beet and Sugar Beet Accessions Measured by Randomly Amplified Polymorphic DNA
... of polymorphic bands per primer ranged from 6 to 12, resulting in a total of 42 polymorphic ...of polymorphic bands per primer ranged from 2 to 13, resulting in a total of 201 ...
6
Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic DNA fingerprinting
... phisms amplified by the eight functional RAPD primers from DNA extracted from two subcultures of ...primer amplified a DNA fingerprint ranging from 5 to 20 bands, over a size range of 100 bp ...
7
Assesment Of Genetic Diversity In Verbesina Encelioides Populations Using Randomly Amplified Polymorphic Dna (rapd) Markers
... two different types of flower structures: 1)that of the rayflorets, which appear as the petals, and 2) that of the disc-floret, which is in the center of the flower head (Hylander and Johnston 1954; Gardner et al. 1956). ...
8
Genetic diversity analysis of endemic species, Garcinia cambogia Gaertn using randomly amplified Polymorphic DNA markers
... random amplified polymorphic DNA (PCR-RAPD) markers were employed to assess genetic diversity in eight Garcinia cambogia ...were polymorphic, showing 99.11% polymorphism. An average of 25.22 ...
5
To study of the genetic variations among the Azospirillum lipoferu isolates using randomly amplified polymorphic DNA (RAPD) marker
... F. DNA extraction protocol followed was according to Sambrook et ...the DNA. The pellet was washed with 70 % ethanol and air-dried. The DNA was dissolved in TE ...
6
Genetic Diversity Among Sugarcane (Saccharum Officinarum L.) Genotypes as Shown by Randomly Amplified Polymorphic Dna (Rapd)
... tralia, America, Asia, and Africa were used in this study (Table 1). One-node cuttings were planted in a mixture of soil and compost (1:1 v/v) contained in a polybag and maintained for 4 months. Watering was routinely ...
11
Analysis of an outbreak of non phage typeable methicillin resistant Staphylococcus aureus by using a randomly amplified polymorphic DNA assay
... Seven (44%) of these 16 colonized patients developed MRSA bacteremia. MRSA isolates with the same characteristics were also found on the hands of one member of the ICU staff. The isolates were untypeable by phage typing, ...
6
Molecular Epidemiological Investigation Using a Randomly Amplified Polymorphic DNA Assay of Burkholderia cepacia Isolates from Nosocomial Outbreaks
... Received 13 January 1999/Returned for modification 27 February 1999/Accepted 21 August 1999 We experienced two Burkholderia cepacia outbreaks over a 1-year period. During this period, 28 B. cepacia isolates were obtained ...
6