Reverse transcriptase-coupled quantitative real-time PCR (RTqPCR)
Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... potential quantitative characteristics in comparison with conventional diagnostic methods but are often still not routinely ...by quantitative nucleic acid sequence-based amplification (QT-NASBA), ...
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Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... RT- PCR assay we have developed can be used for epidemiological studies of the EV-D68 outbreak and for virus monitoring in sub- sequent ...this time whether typing EV-D68 will be useful for patient ...
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Comparison of FilmArray and Quantitative Real Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens
... the 16 specimens that tested negative by qRT-PCR assay, 4 had detectable virus by FilmArray testing. Specimen type, sample volume, handling, and storage are im- portant to consider when interpreting the results of ...
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Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... Advances in the development of fluorophores and nucleo- tide-labeling chemistries have resolved the need for postam- plification manipulations required by conventional RT-PCR and have provided the capability to ...
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS- CoV) to investigate the potential of a hot start RT for improved real-time ...
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Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... Clinical samples. A total of 89 human serum samples were used to evaluate the multiplex RT-PCR for its diagnostic potential. Fifty clinical samples provided by St. George’s University (St. George, Grenada) were ...
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Development of One Step, Real Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E
... TaqMan-based, real-time quantitative reverse transcriptase PCR (qRT-PCR) for the rapid detection and quantitation of human coronaviruses (HCoVs) OC43 and ...the ...
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... compared using serial dilutions of the virus. However, in comparison with conventional RT-PCR, the fluorogenic assay presents many advantages, the most important being a closed system in which the tube is never ...
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Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura virus
... compared using serial dilutions of the virus. However, in comparison with conventional RT-PCR, the fluorogenic assay presents many advantages, the most important being a closed system in which the tube is never ...
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Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... Dengue virus infections continue to be an important public health problem, and rapid, accurate diagnosis of DEN virus in acute infections is crucial for treatment of patients and for effective surveillance and control of ...
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Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... of real-time RT-PCR to quantifica- tion of CCR5 mRNA in MDM and PBL isolated from adult peripheral ...CCR5 real-time RT-PCR and quantified as described ...
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Reverse Transcriptase Pcr Protocol Pdf
... is time well ...assurance. Pcr assay parameters the only be conducive to more likely improve the hospital of patients with pcr protocol is necessary to the amplification plot exponential phase thus ...
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Evaluation of a Rapid and Completely Automated Real Time Reverse Transcriptase PCR Assay for Diagnosis of Enteroviral Meningitis
... The benefits of the Xpert EV assay are the ability to obtain reliable results rapidly, within several hours of spinal fluid collection, and to decentralize the testing process so that rapid turnaround time for ...
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Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York 11944, 1 and Tetracore Inc., Gaithersburg, Maryland 20878 2 Received 3 June 2002/Returned for ...
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Quantification of Substance P mRNA in Human Immune Cells by Real-Time Reverse Transcriptase PCR Assay
... the PCR master mixture (5 l of 1⫻ PCR buffer II, 5 mM MgCl 2 , 300 nM deoxynucleoside triphosphates, 400 nM each primer, ...The PCR buffer contained 5-carboxy-X-rhodamine (ROX) (500 nM) as the ...
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Comparison of a Real Time Reverse Transcriptase PCR Assay and a Culture Technique for Quantitative Assessment of Viral Load in Children Naturally Infected with Respiratory Syncytial Virus
... over quantitative RSV ...of quantitative culture ...fresh quantitative cul- ...titative PCR would avoid this ...over time as measured by ...multiple time points were ...over ...
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Two Novel Real Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination in Platelet Concentrates
... Two real-time reverse transcriptase PCR (RT-PCR) assays performed in a LightCycler instrument were developed and compared regarding specificity and sensitivity by the use of ...
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Real Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages
... Specificity of the NL-N assay. To test whether the primers and probe designed for the NL-N assay were specific for hMPV, we used RNAs from 15 other common respiratory viral agents as templates for real-time ...
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... of time that the virus was ...the real-time RT-PCR assay in amplifying an in vitro-transcribed RNase-resistant RNA tem- plate was independent of the virus species, although assays with ...
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Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
... Statistical analysis. Inter- and intra-assay variances were computed using the C T values, standard deviations, and coefficients of variation of the standard curves. The variance was analyzed by a one-way repeated- ...
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