Reverse Transcriptase (RT) -PCR
Detection of Treponema pallidum by a sensitive reverse transcriptase PCR
... several PCR methods have been developed for the detection of ...sensitive reverse transcriptase PCR (RT-PCR) that targets a 366 bp region of the 16S rRNA of ...
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Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... novel reverse transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P ...
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Single tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis
... as PCR, are widely accepted for the detection of infectious ...a reverse transcriptase PCR (RT-PCR), a positive signal would indicate the presence of a recently viable ...
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Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
... by reverse transcriptase PCR (RT-PCR) with blood drawn into tubes containing EDTA and those obtained by RT-PCR with blood samples in serum separator tubes from 108 individuals ...
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Multiplex Reverse Transcriptase-PCR Assay for Typing and Subtyping of Influenza A (H5 & H9) Virus in Iran
... Multiplex Reverse Transcriptase PCR (RT-PCR), Subtyping INTRODUCTION nfluenza A virus, a member of the Orthomyxoviridae family, infects swine, horses, seals, and a large number of birds as ...
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Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... also assessed in a representative collection of DEN virus iso- lates, other closely related flaviviruses, and arboviruses that may share the same geographical range or mosquito vector. The assay was shown to be DEN virus ...
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Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... of Medicine, Philadelphia, Pennsylvania 19104 Received 21 February 2003/Returned for modification 22 April 2003/Accepted 23 July 2003 CCR5, a  -chemokine receptor, plays an important role in human immunodeficiency virus ...
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Development and Evaluation of Serotype and Group Specific Fluorogenic Reverse Transcriptase PCR (TaqMan) Assays for Dengue Virus
... (TaqMan) reverse transcriptase PCR (RT-PCR) assays were developed for serotypes 1 to 4 and group-specific detection of dengue ...45-cycle PCR at 95 and 60°C that generates a fluorogenic ...
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Two Novel Real Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination in Platelet Concentrates
... real-time reverse transcriptase PCR (RT-PCR) assays performed in a LightCycler instrument were developed and compared regarding specificity and sensitivity by the use of different templates to ...
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Reverse Transcriptase Pcr Protocol Pdf
... of PCR, oron ...specimens. PCR instrument software before the run. PCR kits are designed and validated as a ...button. Pcr protocol pdf download all content depresses the reverse ...
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Typing and Subtyping Influenza Virus Using DNA Microarrays and Multiplex Reverse Transcriptase PCR
... viruses. Reverse transcriptase PCR was used to prepare cDNAs encoding ⬃ 500-bp influenza virus gene fragments, which were then cloned, sequenced, reamplified, and spotted to form a glass-bound ...
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Longitudinal assessment of feline immunodeficiency virus kinetics in plasma by use of a quantitative competitive reverse transcriptase PCR.
... Cats infected with feline immunodeficiency virus (FIV) develop a disease syndrome similar to that caused by human immunodeficiency virus type 1 (HIV-1) infection in humans. HIV-1 replication has been shown to correlate ...
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Detection of Colorado tick fever virus by using reverse transcriptase PCR and application of the technique in laboratory diagnosis
... by reverse transcriptase ...The reverse transcriptase PCR method is a promising tool for the early diagnosis of CTF viral infection, or for ruling out CTF virus as the etiologic agent, ...
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Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York 11944, 1 and Tetracore Inc., Gaithersburg, Maryland 20878 2 Received 3 June 2002/Returned for ...
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Real Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages
... real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and ...
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... real-time reverse transcriptase PCR has been developed for the rapid detection and identifica- tion of eight medically important flaviviruses from laboratory-reared, virus-infected mosquito ...
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Comparison of Enzyme Immunoassay and Reverse Transcriptase PCR for Identification of Serotype G9 Rotaviruses
... RT- PCR genotyping were also G9 by EIA, thus confirming that serotype G9 rotaviruses were a common cause of rotavirus infections of neonates in India in ...
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Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... While there are no specific treatments for EV-D68 and cur- rently no antiviral targets available, rapid and accurate diagnosis of current and future EV-D68 infections is of great concern to clinicians and public health ...
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Rapid diagnosis of Argentine hemorrhagic fever by reverse transcriptase PCR based assay
... A patients) were positives, clinically diagnosed as AHF or FSUE, and 28 (Table 1, group B patients) were negatives. However, in the last group, some patients had a clinical diag- FIG. 2. Validation of RT-PCR. The ...
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International Collaborative Study To Compare Reverse Transcriptase PCR Assays for Detection and Genotyping of Noroviruses
... NLV strains, should be significantly increased to detect all currently known genotypes. This finding implies that the assays should be used with caution: while they may work well with outbreak specimens (multiple ...
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