RNA-sequencing
Single-cell RNA-sequencing of the brain
... and sequencing; CNS: central nervous system; COP: committed oligodendrocyte precursors; CPM: counts per million mapped reads; CV: coefficient of variation; ERCC: External RNA Con‑ trols Consortium; FACS: ...
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Deep RNA Sequencing of the Skeletal Muscle Transcriptome in Swimming Fish
... Deep RNA sequencing (RNA-seq) was performed to provide an in-depth view of the transcriptome of red and white skeletal muscle of exercised and non-exercised rainbow trout (Oncorhynchus mykiss) with ...
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RNA sequencing identifies novel non-coding RNA and exon-specific effects associated with cigarette smoking
... RNA sequencing (RNA-seq) facilitates the ability to look at more complex regulatory phenomena such as isoform- switching, alternative promoter usage, and exon inclusion/ exclusion ...of RNA ...
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The design and statistical analysis of single-cell RNA-sequencing experiments
... and RNA-sequencing technologies have expanded rapidly in both throughput and accuracy within the last ...single-cell RNA- sequencing (scRNA-seq) experiments, specifically as it relates to ...
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Single cell RNA sequencing to dissect the molecular heterogeneity in lupus nephritis
... RNA sequencing (RNA-seq) is an important method defining the transcriptomes from total RNA extracted from whole tissues, cell populations, and more recently even single cells, providing ...
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RNA Sequencing Analysis of Messenger RNA/MicroRNA in a Rabbit Aneurysm Model Identifies Pathways and Genes of Interest
... RESULTS: A total of 9396 genes were analyzed by using RNA sequencing, 648 (6.9%) of which were found to be significantly differentially expressed between the aneurysms and control tissues (P ⬍ .05; ...
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PARSES: A Pipeline for Analysis of RNA-Sequencing Exogenous Sequences
... generation sequencing technologies ...from RNA-Seq experiments, solving the problem of mapping reads back to the host organism's genome [2] ...interrogate RNA-Sequencing experiments for ...
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Quantifying expression variability in single-cell RNA sequencing data
... single-cell RNA sequencing (scRNA- Seq) technologies, new computational strategies to quantify variability were introduced ...single-cell sequencing and imaging technologies and comment on robust ...
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Detection and classification of peaks in 5' cap RNA sequencing data
... Results: We develop a two-stage approach consisting of a fast and simple algorithm based on a sliding window with Poisson null distribution for detecting the genomic locations of peaks, followed by a linear support ...
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limma powers differential expression analyses for RNA-sequencing and microarray studies
... of RNA sequencing (RNA- seq) ...for RNA-seq as well. These capabilities allow users to analyse both RNA-seq and microarray data with very similar ...
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Genetic diagnosis of Mendelian disorders via RNA sequencing
... transcriptome sequencing of an unaffected tissue can help ...performed RNA-seq on patient derived dermal fibroblast cell ...preclude RNA-seq of unaffected tissues from revealing the causative defect ...
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Visualization methods for genealogical and RNA-sequencing studies: Pertinence, software, and applications
... of RNA-sequencing ...in RNA- sequencing data ...kidney RNA samples required more than library size scaling normalization and that it profited from TMM normalization (Marioni et ...
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A telescope for the RNA universe : novel bioinformatic approaches to analyze RNA sequencing data
... big RNA-protein complex that excises certain regions of pre-mRNA (mainly annotated as introns) and joins the remaining regions (mainly annotated as ...employing RNA sequencing technology indicated ...
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Novel Bayesian methodology for the analysis of single-cell RNA sequencing data.
... decade, RNA sequencing technologies have been instrumental in trans- forming our knowledge and understanding of ...Currently, RNA sequenc- ing experiments fall into one of two categories: bulk ...
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Deep RNA Sequencing Reveals Complex Transcriptional Landscape of a Bat Adenovirus
... deep RNA sequencing was used to analyze the transcriptome at five time points follow- ing the infection of a bat AdV in a kidney cell line derived from a myotis bat ...deep RNA sequencing pro- ...
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The Application and Challenges of RNA-Sequencing to the Study of Circadian Rhythms
... splicing, RNA editing, allele specific expression, and identify novel transcripts, both coding and non-coding RNAs [58, 142, ...based RNA expression analysis, the flexibility of RNA-seq has allowed ...
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A RNA-Sequencing approach for the identification of novel long non-coding RNA biomarkers in colorectal cancer
... by RNA sequencing ...conclusion, RNA-seq identified numbers of novel lncRNAs dysregulated in ...of RNA-seq to discover novel lncRNAs involved in human carcinogenesis, which may serve as ...
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limma powers differential expression analyses for RNA-sequencing and microarray studies
... of RNA sequencing (RNA-seq) ...for RNA-seq as well. These capabilities allow users to analyse both RNA-seq and microarray data with very similar ...
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RNA sequencing reveals a depletion of collagen targeting microRNAs in Dupuytren’s disease
... sequencing reads. The majority of the differentially expressed microRNA reads were found to be concentrated amongst a relatively small group of differentially expressed microRNAs (Fig. 4). Notably the microRNAs ...
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PBMC fixation and processing for Chromium single cell RNA sequencing
... causes RNA degradation [9, 16] and loss [11] in many types of cells, especially primary cells such as ...when RNA loses its integrity, we broke up the methanol fixation and processing proce- dure into three ...
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