[PDF] Top 20 Analysis of Herpes Simplex Virus Type 1 DNA Packaging Signal Mutations in the Context of the Viral Genome

... Table 1) confirmed and extended our previous obser- vations made using the amplicon assay ...in DNA cleavage and ...of virus progeny, c1, c3, and c4 (substitutions of the pac2 GC element, unconserved ... See full document

... XbaI DNA fragments encompassing the entire HSV-1(F) ge- nome were tested for the ability to rescue replication at 39°C using methods described previously ...Table 1, the XbaI E fragment (diagrammed ... See full document

... the viral thymidine kinase (TK) gene and ligated to the Uc-DR1-Ub ...the packaging signal into the TK gene of an HSV-1 genome from which all copies of the a sequence had been ...a ... See full document

... HSV-1 DNA encapsidation remains rel- atively poorly understood, and many fundamental issues, such as the polarity of packaging, remain to be ...during DNA packaging only one terminus, ... See full document

... LMW DNA ex- tracted from both types of infected cells by Southern blot analysis, using a loxP-a-loxP probe, as previously ...of DNA molecules containing the a signal, previ- ously observed ... See full document

... the herpes simplex virus type 1 UL25-null mutant KUL25NS have shown that the capsid- associated UL25 protein is required at a late stage in the encapsidation of viral ...the ... See full document

... the packaging machinery at the portal during and after inser- tion of the genome into the ...the DNA-containing capsid (32). The HSV-1 genome, like the ␾ 29 genome, is inserted ... See full document

... Cellular DNA replication in eukaryotic nuclei occurs in dis- crete domains called replicons (26, ...in DNA replication suggests that replicon formation may be ...manipulated, herpes simplex ... See full document

... this virus since it lacks the a sequence, whereas the homologous recombination model predicts that all four isomers would still be generated in this virus because it retains other regions of significant se- ... See full document

... HSV-1 genome isomerization ...out DNA inversion-type ...and packaging machinery ...wild-type virus; however, high-frequency inversion did not occur within pL D R- 193 when ... See full document

... for packaging of mature, linear ...the genome occurs at the time of encapsidation and that mutations of the capsid proteins that abolish encapsidation also prevent the formation of genomic termini ... See full document

... for packaging (including the portal protein) are mainly structural components of the ...for DNA packaging are replication-generated concatemers, and the DNA is cleaved into genome-size ... See full document

... The herpes simplex virus type 1 (HSV-1) UL25 gene encodes a minor capsid protein, pUL25, that is essential for packaging the full-length viral ...missense ... See full document

... a packaging signal, and we also showed that it functions with an efficiency similar to that of larger fragments containing one or two copies of the complete a sequence, including the highly reiterated DR2 ... See full document

... certain mutations or inhibitory compounds may result in a phenotype in which herpesvirus DNA is cleaved and packaged but is then either lost from capsids or retained in capsids that do not protect the ... See full document

... Standard virus stocks were prepared by passaging HSV ANG four 423 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest DNA-DNA reassociation kinetics of herpes simplex virus[r] ... See full document

... mature herpes simplex virus type 1 (HSV-1) infectious particle has a complex structure consisting of a DNA core inside an icosahedral capsid, a protein layer referred to ... See full document

... stranded DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-length genomes by a complex mechanism that is tightly coupled to DNA insertion into a preformed capsid ... See full document

... progeny virus (11, 19, 46, 53, ...any DNA-containing capsids appear in the cytoplasm ...in genome maturation, which in turn affects the stability of DNA-contain- ing ...the DNA which ... See full document

... The cloned fragments were identified by digestion of the chimeric plasmid DNA with restriction enzymes EcoRI or EcoRI and BglII followed by comparison of their electrophoretic mobilities[r] ... See full document