[PDF] Top 20 Characterization of the DNA-binding properties of herpes simplex virus regulatory protein ICP4.
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Characterization of the DNA-binding properties of herpes simplex virus regulatory protein ICP4.
... DNAbinding immunoassays with fraction VIII and portions of the HSV genome revealed stable complexes that contained ICP4 and specific sequences in the promoters for viral genes encoding I[r] ... See full document
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Isolation of herpes simplex virus regulatory protein ICP4 as a homodimeric complex.
... DISCUSSION We report here the isolation and characterization of a soluble form of native ICP4 from the nuclei of Vero cells infected with HSV-1HFEM.. The isolation conditions required th[r] ... See full document
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Herpes Simplex Virus Type 1 ICP4 Promotes Transcription Preinitiation Complex Formation by Enhancing the Binding of TFIID to DNA
... the ICP4 protein on the formation of transcription ...of ICP4 in PIC formation, we examined the interaction of the GTFs with a promoter template immobilized on a magnetic solid support (1, 11, 34) in ... See full document
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Relationship between TATA-binding protein and herpes simplex virus type 1 ICP4 DNA-binding sites in complex formation and repression of transcription.
... are protein-protein contacts between ICP4 and the DB complex on ...the binding of ICP4 to DNA is transmitted down the helix to the TATA box such that TBP binds with higher ...The ... See full document
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Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.
... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document
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Identification of a Motif in the C Terminus of Herpes Simplex Virus Regulatory Protein ICP4 That Contributes to Activation of Transcription
... binding site in the LAT ...Gal4 binding sites upstream of the E1b TATA box and the luciferase gene, has been previously described ...simian virus 40 (SV40) small-t-antigen intron and poly(A) ... See full document
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Requirements for activation of the herpes simplex virus glycoprotein C promoter in vitro by the viral regulatory protein ICP4.
... 4, an ICP4-binding site in the leader is required for activation if additional downstream binding sites are removed; ii the downstream ICP4-binding sites, which are located in the tk gen[r] ... See full document
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Characterization of two conformational forms of the major DNA-binding protein encoded by herpes simplex virus 1.
... SPANG Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115 We have resolved two electrophoretic species of the major DNA-binding protein[r] ... See full document
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A second-site revertant of a defective herpes simplex virus ICP4 protein with restored regulatory activities and impaired DNA-binding properties.
... ICP4 has been shown to specifically interact with lower-affinity sites that differ significantly from the consensus sequence found in the vicinity of early and late gene promoters 23, 25[r] ... See full document
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Purification of the herpes simplex virus type 1 65-kilodalton DNA-binding protein: properties of the protein and evidence of its association with the virus-encoded DNA polymerase.
... 8 Purification of the Herpes Simplex Virus Type 1 65-Kilodalton DNA-Binding Protein: Properties of the Protein and Evidence of Its Association with the Virus-Encoded DNA Polymerase MICHA[r] ... See full document
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Mutations that specifically impair the DNA binding activity of the herpes simplex virus protein UL42.
... and DNA by virtue of its ability to bind both the catalytic subunit and the DNA ...such DNA was greater than that of either protein alone (18, ...replicative DNA polymerase d in ...the ... See full document
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... native protein. Once such conditions were established, the modified protein would subsequently be characterized, and if its prop- erties were found to be significantly different from those of the unmodified ... See full document
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Properties of the novel herpes simplex virus type 1 origin binding protein, OBPC.
... HSV-1 DNA replication, and of the potential role of OBPC in this process, is a consideration of the structure of replicative inter- ...HSV-1 DNA is likely replicated by a theta mechanism early in infec- ... See full document
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Initial characterization of the membrane-associated form of ICP4 of herpes simplex virus type 1.
... of herpes simplex virus type 1 (HSV-1) and plays an essential role in regulating the expression of all three kinetic classes of HSV-1 genes (5–7, 10, 19, ...of ICP4 are not viable in cells ... See full document
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Identification of a promoter-specific transactivation domain in the herpes simplex virus regulatory protein ICP4.
... the ICP4 binding sites in the gD pro- moter on activation by native ICP4 and an ICP4 mutant that lacks the N-terminal ...well-defined ICP4 binding sites in the upstream portion ... See full document
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Activities of heterodimers composed of DNA-binding- and transactivation-deficient subunits of the herpes simplex virus regulatory protein ICP4.
... Despite the transcriptional defect of the vil2 and vil3 polypeptides observed in infected Vero cells, transcription of the gene for tk was induced upon infection of X25 cells with either[r] ... See full document
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Separation of primary structural components conferring autoregulation, transactivation, and DNA-binding properties to the herpes simplex virus transcriptional regulatory protein ICP4.
... Mutant ICP4 alleles transiently expressed in CV-1 cells were assessed for the ability to i form protein-DNA complexes with a DNA fragment containing the ICP4 transcription initiation sit[r] ... See full document
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DNA-Dependent Oligomerization of Herpes Simplex Virus Type 1 Regulatory Protein ICP4
... the protein did not affect B complex formation, and (iii) an N-terminal domain mutant formed heterotetramers with WT ...specific ICP4 binding site and a downstream nonspecific stretch of DNA ... See full document
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In vitro characterization of a thermolabile herpes simplex virus DNA-binding protein.
... 31 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The major herpes simplex virus DNA-binding protein, ICP8, was purified from cells infected with the herpes simplex vi[r] ... See full document
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Physical and functional domains of the herpes simplex virus transcriptional regulatory protein ICP4.
... Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.. Activation of immediate-early, early, and[r] ... See full document
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