[PDF] Top 20 Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
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Comparison of Real Time Reverse Transcriptase PCR Assays for Detection of Swine Hepatitis E Virus in Fecal Samples
... These samples originated on 86 farms located in 12 ...The fecal samples were ob- tained from different age groups of pigs: suckling (1 to 2 weeks of age), nursery (3 to 7 weeks of age), and ... See full document
7
Molecular Epidemiology of Norovirus Outbreaks in Norway during 2000 to 2005 and Comparison of Four Norovirus Real Time Reverse Transcriptase PCR Assays
... our real-time PCR ...four fecal samples containing different NoVs (two GI and two ...TaqMan assays, except for the annealing temperature that was in- creased to 56°C in assay B ... See full document
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Comparison of Performance Characteristics of Three Real Time Reverse Transcription PCR Test Systems for Detection and Quantification of Hepatitis C Virus
... qualitative assays (Ampli- cor HCV; Roche Diagnostics, Indianapolis, IN; and TMA- based Versant; Bayer HealthCare, Berkley, CA) and a higher dynamic range than the quantitative available assays ...for ... See full document
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Development of an Internally Controlled Real Time Reverse Transcriptase PCR Assay for Pan Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays
... A further benefit of the pan-DENV assay is the multiplex de- tection of RNase P, which serves as a heterologous intrinsic IC. Of the method comparisons mentioned previously, only the conven- tional RT-PCR ... See full document
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Comparison of FilmArray and Quantitative Real Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens
... the virus in the analyte preparations than the ability of the test systems to detect ...the assays, but the EUA documentation includes results of extensive studies that were performed with the help of the ... See full document
5
Development of a pan Simbu real time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
... pan-Simbu real-time reverse transcript- ase (RT) PCR system was developed based on two con- served sequence regions of the L-segment facilitating the amplification of a 279 base pair (bp) ... See full document
9
Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... duplex real-time PCR using two pairs of primers and two probes targeting the S re- gion; however, the assay was only used to screen for HBV DNA, and it has not been demonstrated that mu- tations in ... See full document
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Design and Performance of the CDC Real Time Reverse Transcriptase PCR Swine Flu Panel for Detection of 2009 A (H1N1) Pandemic Influenza Virus
... Influenza Virus Real-time RT-PCR Detection and Characterization Panel (CDC rRT-PCR Flu Panel) ...for detection of the influenza A virus matrix gene as the CDC ... See full document
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Two Novel Real Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination in Platelet Concentrates
... The bacterial contamination of blood products remains a persistent but often ignored problem in transfusion medicine. The incidence of platelet bacterial contamination is approxi- mately 1 per 2,000 to 3,000 units (13). ... See full document
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Design and Validation of Real Time Reverse Transcription PCR Assays for Detection of Pandemic (H1N1) 2009 Virus
... three real-time RT-PCR assays based on the available se- quences for pandemic (H1N1) 2009 virus recovered at the outset of the ...outbreak. Comparison of the performance char- ... See full document
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Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... outperformed virus isolation, which is considered the diagnostic standard for CSF (8, 24, ...before detection by virus isolation ...early detection of ...by virus isolation with SK6 ... See full document
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TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... and swine HEV, both consisting of three open reading frames (ORFs) and two short noncoding regions ...and swine HEV genome, and they share only approximately 50 to 60% sequence identity (2, 15, 17, ...for ... See full document
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Comparison of Real Time PCR and Antigen Assays for Detection of Hepatitis E Virus in Blood Donors
... early detection of viral infection prior to the occurrence of IgM antibodies, would enhance the management of HEV ...the detection of anti-HEV IgM (Wantai HEV-Ag assay ...the detection of HEV-Ag ... See full document
7
Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR
... viral detection methods may be in the detection of hRSV infection in older children and adults, particularly in cases of ...detect virus in a clinical spec- imen might be due to the presence of ... See full document
5
Comparison of Real Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp paratuberculosis in Bovine Fecal Samples
... the detection of infected animals and animal ...incubation time (12 to 16 weeks). Enzyme- linked immunosorbent assays can be performed in a few hours, but their sensitivity is estimated at 45% since ... See full document
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Real Time PCR for Detection and Differentiation of Entamoeba histolytica and Entamoeba dispar in Fecal Samples
... of PCR with Entamoeba ...fecal samples. Thus, results of ameba cul- ture were compared with those of PCR (Table ...181 fecal samples, which were collected during a study in ... See full document
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Detection and survival of SARS in human stool, urine, wastewater and sludge
... Ahmed, W., Angel, N., Edson, J., Bibby, K., Bivins, A., O’Brien, J. W., Choi, P. M., Kitajima, M., Simpson, S. L., Li, J., et al. (2020). First confirmed detection of SARS- CoV-2 in untreated wastewater in ... See full document
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Multicenter Comparison of Different Real Time PCR Assays for Quantitative Detection of Epstein Barr Virus
... Epstein-Barr virus (EBV), like all members of the human herpesvirus family, persists in the host after primary infection and may reactivate at any time. EBV causes substantial mor- bidity especially among ... See full document
7
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... multiplex real-time PCR NHS assay using specimens from routine surveillance for invasive disease caused by ...NVTM samples) were used to validate the NHS assay in multiplex and singleplex ... See full document
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Development of Multiplex Real Time Reverse Transcriptase PCR Assays for Detecting Eight Medically Important Flaviviruses in Mosquitoes
... for detection of DENV-1 to -4 was demonstrated in our previous publication ...of virus-specific probes; and optimized a multiplex RT-PCR for the simultaneous detection of viral RNA genomes of ... See full document
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