[PDF] Top 20 Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)

... Amelia J. Armstrong 1* , Christine L. Dudgeon 1 , Carlos Bustamante 2 , Michael B. Bennett 1 and Jennifer R. Ovenden 1 Abstract Objective: Limited sample sizes are often a problem for species of conservation concern when ... See full document

... highly polymorphic for cultivated peanut in a previous study, long TC repeats [10] For this, sequences were obtained from an enriched genomic ...described markers, and assemble a compact database of ... See full document

... The testing of 144 primer pairs resulted in the selection of 34 polymorphic loci that could be reliably scored and showed consistent amplification success. We selected a final set of 10 loci for L. johnii, 11 loci ... See full document

... more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important ...molecular markers, genic microsatellite markers ... See full document

... hundred microsatellite loci were identified in next generation shotgun sequences from two African forest elephants, of which 53 were considered suitable for ...Twenty-three microsatellite markers ... See full document

... SSR markers were applied to analyze the genetic diversity among 15 acces- sions of Perilla ...average polymorphic information content (PIC) was ...SSR markers of Perilla species reported in this ... See full document

... highly polymorphic microsatellite ...novel microsatellite markers for ...the markers on samples from across the pantropical distribution of the species to ... See full document

... The development of new microsatellites for ...new microsatellite loci could prove useful in other economically important mite species like Phytoseiidae and other ... See full document

... The development of new markers, pivotal to future genetic–based studies, is therefore ...11 polymorphic markers —selected and tested in samples from two wild study sites— that can be used to ... See full document

... SSR markers in modern genetic studies, microsatellite development and research on Dill is limited due to no or very less prior sequence information high expense and time consumption (Fu et ...few ... See full document

... SSR markers that were polymorphic, generated reproducible amplifications, were easy to score, had low levels of allele dropouts, and lacked null ...those microsatellite markers that are not ... See full document

... However, development of novel microsatellite markers is expensive and quite laborious task ...of microsatellite markers and identification of chloroplast microsatellites using universal ... See full document

... 2012) and whole genome re-sequencing (e.g. Yang et al. 2015) have been consistently applied depending on the purpose of the research and on a budget. We designed a panel of 13 multiplexed microsatel- lite markers ... See full document

... *For correspondence. E-mail: R. Srirama, [email protected]; G. Ravikanth, [email protected]. Received 1 September 2017; revised 21 December 2017; accepted 11 January 2018 Abstract. Phyllanthus emblica and P. ... See full document

... of polymorphic microsatellite markers for ...candidate microsatellite markers were identified and characterized from genomic ... See full document

... oped polymorphic microsatellite loci from Taiwan cow-tail fir to estimate the population structure within the species and to identify distinct evolutionary units within the popu- ...the ... See full document

... threat. Microsatellite markers can help us understand the genetic resources of ...total microsatellite loci, followed by pentanucleotides ...the polymorphic fragments and the number of alleles ... See full document

... the microsatellite isolation process to be streamlined, the primer evaluation step has seen few improvements other than the introduction of the M13 tailed system ...pre-selecting markers for variability in ... See full document

... less polymorphic than other microsatellite marker types, but have the benefit of an efficient and economic method and reduced occurrence of null alleles because the DNA sequences flanking SSRs from tran- ... See full document

... in microsatellite loci was ...the microsatellite repeats were designed with one primer in the set fluorescently ...each microsatellite locus for all isolates. Polymorphic microsatellite ... See full document